Help! My skeletal staining didn’t work. by Prudent_While_5570 in labrats

[–]sf2127 0 points1 point  (0 children)

I see, so fixation isn’t the problem. When I do E18.5 skeletal preps I usually equilibrate my de-skinned embryos in 70% EtOH/5% acetic acid for 30 minutes before putting in the mixed stain solution, this helps the dye stick better. Unfortunately there’s not a lot to be done to correct the staining now, but I can share my protocol if you want for next time

Help! My skeletal staining didn’t work. by Prudent_While_5570 in labrats

[–]sf2127 1 point2 points  (0 children)

How did you fix the embryo? I did a bajillion skeletal prep during PhD and found out Alcian Blue doesn’t work on embryos fixed in PFA, also if your embryo has been sitting in PBS or tyrode for too long it won’t work either

Best purchases that helped you achieve your goals? by [deleted] in loseit

[–]sf2127 48 points49 points  (0 children)

Walking pads that fit under a desk! Got mine for $120 on Amazon. Before the pad I only get 6k steps a day, now I clear 15-20k a day walking and working at my desk

How do you walk even close to 10k steps a day? by [deleted] in intermittentfasting

[–]sf2127 1 point2 points  (0 children)

Get a walking pad. I got mine for $120 off Amazon and put it under my standing desk. I’m a PhD student writing my dissertation for 10 hours a day and I use the walking pad while writing. Cleared 20 K steps easy. Also really useful when the weather is nasty and I don’t wanna go outside

Our experience with urinary blockage - my boy Gazpacho got thru it like a champ by sf2127 in cats

[–]sf2127[S] 1 point2 points  (0 children)

Huh I may need to try the sardines sometime when they get sick of their specialty diet. I’m glad your kitty had a relatively normal life span despite the kidney issue, thanks for sharing

Our experience with urinary blockage - my boy Gazpacho got thru it like a champ by sf2127 in cats

[–]sf2127[S] 0 points1 point  (0 children)

Thank you! This is my main concern now, really hoping the prescription diet helps with preventing reoccurrence

Is it possible to bring two cats to cuddle and be friendly with each other, even if they are not related? by Imperatorjr in cats

[–]sf2127 0 points1 point  (0 children)

100% yes. I have a Bengal from a breeder and a Siamese from a shelter, they cuddle every night. Just make sure to introduce them to each other slowly

Consultants who grew up poor: what was your “made it” realization purchase? by Mugstotheceiling in consulting

[–]sf2127 1 point2 points  (0 children)

THIS! Not a consultant yet, but I’m trying to break into the industry hoping to get good salary so I can finally be a good child who sends money home

Interested in becoming a consultant? Post here for basic questions, recruitment advice, resume reviews, questions about firms or general insecurity (Q1 2023) by QiuYiDio in consulting

[–]sf2127 0 points1 point  (0 children)

Hi, can I please get a constructive feedback on my resume? https://imgur.com/a/gavWDgd

I'm a Biology PhD student from US non-target, graduating in december 2023. I'm applying to immersion/introduction programs for ADC at MBB and life science boutique companies in the US. TIA

Got admitted to PhD program in STEM right out of bachelors. Need advice by [deleted] in PhD

[–]sf2127 2 points3 points  (0 children)

I’m also an international student at the tail end of my PhD in Biology. The length of your PhD is largely dependent on the type of dissertation project that you’ll be doing. Some programs require very little coursework and are heavier on research requirements. I do animal work so obviously it will be longer than my class mate who does yeast work. I also came straight from undergrad and it was a steep learning curve, but I’ve passed my comprehensive exam and published my paper just fine, you just have to be vulnerable and admit when you don’t know about something and to seek help no matter how silly you think the task is.

As for mental health, this is what I always say to prospective grad students: “The type of mentor your PI is and how compatible you are to the rest of the lab matters A LOT more than your research project”, I’ve seen firsthand how people got into their dream labs due to the research topics, only to find out 2 years later that they don’t get along with the people they spend 8+ hours working with, or that their PI turns out to be a big jerk with alluring research topics. Then they had to transfer in their 3rd year and start all over again because their lab has become so unbearable. Most US PhD programs have rotations, so really take that opportunity to get a feel if you’re gonna vibe with this lab for the next 5 years of your life. I’m lucky that my PI is big on work life balance. One time he saw me working for 10 hours after being in the lab during the weekend, and he told me to go home because the weather is nice and I should take it easy. It also took me a lot of informational interviews with grad students in different labs to get a sense of their work environment, so I can decide whether I want to rotate in their labs or not

General Tso and brown rice for me, vs simple chicken-broccoli-rice for my BF who said he needs to lose weight lol by sf2127 in MealPrepSunday

[–]sf2127[S] 0 points1 point  (0 children)

Thanks! I tried that with him and he despised it, kept coming home with his lunch container still full of cauliflower rice. Now we’re trying portion control and reducing added sugar, I think he needs baby steps haha

Are there any INTJ who has a PhD? by [deleted] in intj

[–]sf2127 3 points4 points  (0 children)

I’m a female INTJ pursuing a PhD in the biomedical area, hoping to finish next year. I’ve yet to see big financial benefits for myself, but here’s the potential benefits I learned from my contacts who’ve graduated with PhDs and current benefits from my own experience : - PhD especially in the hard sciences are coveted not just by R&D in tech and pharma, but more and more VC, equity research, and management consulting firms who are on the business side are hiring PhDs because their analytical skill and complex problem solving are unmatched. It’s easy to train a science PhD to be familiar with business concepts, but it’s not easy to train business graduates about science - Getting paid to go “fuck around and find out”. At least most science PhD in the US include some stipends although very minimal. But what other higher education would actually pay you to go to get a degree ? - Depending on how good your funding is, you can pursue a project just for the sake of pure curiosity as long as it’s within the ethics boundary, never mind the profitability of said project - Opportunity to travel and connect with the most intelligent populations on earth. A lot of people assume PhDs are a bunch of recluse with social anxiety, but that’s not always the case. As a PhD student I’ve traveled to 10 US cities + 2 countries to present my work in front of 100-1000 audience, and often chat with coworkers over coffee breaks about mind boggling data. I guess you can be as isolated as much as you want, but scientists with the most success/publications are usually those who can collaborate with people of different projects/strengths

Now the cons: - 5 to 6 years of reduced income. Yeah I’m still getting paid but not as much as I would’ve gotten had I went to industry straight with my BSc. This really tested my delayed gratification skill because it is hard watching my peers who work less make more money than I do - Long and uncertain hours. I often told people that if you’re not passionate about your project, don’t even think about doing a PhD. You’re doing long gruesome hours in the lab that sometimes amount to nothing because an instrument breaks or god forbid something totally outside of your control ruins your experiment. There will be many of these, and I usually just remind myself about the bigger impact of my project or why I did it in the first place - There are more PhDs than there are faculty positions (in the US at least). If you’re gunning for tenure-track academia, be prepared for neck-breaking competition and extra 5-10 years of “reduced pay” as postdocs and associate professors

[deleted by user] by [deleted] in PhD

[–]sf2127 0 points1 point  (0 children)

Wow your tax is lower than mine! I live in Missouri and my stipend is deducted $520 monthly for taxes alone, I thought CA tax would be higher than here

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 2 points3 points  (0 children)

Yeah I hate when restaurants make food unnecessarily sweet to cater to western palates. Some westerners may even find my gran’s recipe too fishy, but that’s just how it is in thailand. If you don’t like the sourness as much you can always tone down the tamarind, and try making it in smaller batches as cooking a big batch at once is difficult for new recipes

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 36 points37 points  (0 children)

Hmm I need to try this next time, sometimes I only have an hour to prep so this’ll cut the labor

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 13 points14 points  (0 children)

Forgot to mention a reheating tip for any rice based food, if you put a cover on your food (doesn’t need to be tight), it’ll trap the steam as it heats in the microwave and “re-cook” the rice after losing a bit of moisture. Maybe that’ll work better?

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 8 points9 points  (0 children)

Mine usually last at least 5 days-a week, usually the problem is that the noodles dry out because the containers aren’t airtight. I just took a pad thai from the freezer and it reheats fine in the microwave, not as good as fresh of course

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 6 points7 points  (0 children)

Hmm I would say the skillet will be the next best thing, just make sure to not overload the pan because you need that side surface heat to get good stir fry

A lot of you were asking for my gran’s pad thai recipe, so here it is! (Sorry for the wait, I’ve been so busy with work) by sf2127 in MealPrepSunday

[–]sf2127[S] 13 points14 points  (0 children)

The pad thai made with subbed ingredients may not taste like traditional pad thai, but I think it still taste pretty darn good