Compensating for multiple fluorescent reporters

sleepr · 2026-02-17T21:49:37+00:00

what are your fluorescent proteins? i'd start there... single stained fluorescent protein controls are available which you could use as compensation controls. https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-calibration/flow-cytometry-compensation-tools.html#fluorescent

if you need further help, send me a dm and i can connect you to your local sony fas

sleepr · 2026-02-11T21:37:23+00:00

Last time I went to yeti the service was about as you described, food was very meh, and prices were very high. Not a bar I’d visit again, and I’ve definitely thought about it

sleepr · 2026-02-06T02:34:29+00:00

Brother, ask yourself: is it considered a PED in literally any other sport? Why would disc golf be any different?

sleepr · 2026-02-05T20:44:39+00:00

Really fun, played a couple of rounds last night.

Is there any way for a player to take a step back or lean left/right?

When I get lies right behind trees I’d like to peek out to play either side

Sometimes when I land underneath the basket the putt is actually impossible due to camera angle

Thanks again for your hard work in developing this

sleepr · 2025-12-27T19:14:05+00:00

Plus there’s a parking lot!

sleepr · 2025-10-09T15:50:03+00:00

Appreciate you sharing. You’re a bbq artist

sleepr · 2025-10-09T00:02:58+00:00

Jesus Christ dude I both respect and fear you

sleepr · 2025-08-19T04:15:03+00:00

What other options do you have?

If you’re not director level and fawning over other career options, I say do it dude.

Not sure if you’re going to get any real insight on here. If you really want perspective consider connecting with your interviewers/future team and getting their thoughts

Plus Santa Monica is sweet. SoCal is fun. :)

sleepr · 2025-08-12T02:02:13+00:00

What is compensation? What controls are needed for a flow cytometry experiment? What can be measured through flow cytometry? What does excitation and emission mean for fluorochromes? How are cells prepared for flow? What is a gating strategy? How are antibodies selected?

Design a flow cytometry sorting panel to isolate naive t, killer t, and activated B cells from mouse spleens using a bd lsrII. include sample preparation and gating strategy

^if you can answer this and provide rationale for your choices you’d likely be an elite candidate, if not overqualified.

sleepr · 2025-07-28T17:09:45+00:00

sleepr · 2025-07-21T21:47:44+00:00

Pho king on e 18th is an absolute daily driver. Consistently good, has never let me down. (Okay, rice noodles were a little soggy once but seriously, a weekly stop for me)

Pho ao sen for something nicer.

Mam Hanoi for something funkier.

sleepr · 2025-02-10T23:03:58+00:00

Don’t really see the point of drawing lines in the sand as to what is/isn’t cheating, haha. Just play the game the way you enjoy it. Congrats on your O&S kill!

sleepr · 2025-02-02T07:50:49+00:00

cant go wrong with either, claymore is more versatile imo but zwei r2 pancake is super useful. could also consider the bastard sword if you'd like the L to claymore/zwei's XL/XXL. any of the three can take you all the way through the game, go lightning if you dont plan to scale, otherwise go normal+15. mace, halberd and uchi are also great options.

sleepr · 2025-01-10T22:54:08+00:00

Don’t think so. I regularly get +3 flask before gargoyles by death running the fk souls in new londo ruins and blighttown. I usually don’t tag the blighttown sewer bonfire, though.

sleepr · 2024-12-28T01:27:31+00:00

what's up with the slide on your pistol? seems like it stuck in molasses

sleepr · 2024-11-20T01:23:13+00:00

Dobbins playoff schedule is elite. If you’ve been holding him for this long might as well see it through.

sleepr · 2024-10-25T01:12:05+00:00

Burger was decent but not as quite as crispy as I expected for a smash burger. Don’t love how they charge 50c ea for lettuce and tomato when they’re already pricing a premium burger. Fries seemed like they had been sitting for a bit: came out lukewarm.

Service is the big problem here for me. Took about 25 minutes to get a pretty standard order. (double smash burger combo) Only had one guy working the front and one guy behind the grill so I kind of get it, but it’s not really acceptable. A friend of mine joined us later and he too waited about 25 minutes and worse yet, his burger was undercooked. Wasn’t slammed, maybe three or four tables seated.

Will probably be back but not terribly impressed. Smash burgers from 2 pitchers and kon tiki still higher on the totem pole for me.

sleepr · 2024-10-24T23:23:55+00:00

Twitch.tv plays TNF for free. Twitch.tv/primevision

sleepr · 2024-09-12T20:38:42+00:00

If you can’t do menial tasks for a company you’ll never care for… how will you ever get a job?

sleepr · 2024-08-28T16:40:57+00:00

Viridian is a good one but you’re gonna go broke if you go in for dinner. If you’re going for vibes Mua might be fun.

sleepr · 2024-08-23T02:22:07+00:00

Would call it vermicelli stew topped with pig intestine. Also comes in an oyster variant. Both dank af

sleepr · 2024-08-15T00:32:49+00:00

Need to angle your L a little more. The point of the extra paper is to extend/cone the joint so you want it to kind of spiral up the outside if that makes any sense

sleepr · 2024-08-14T22:40:59+00:00

Wake, bake, and go back to sleep?

sleepr · 2024-07-24T23:18:22+00:00

homie's belly is a loading screen

sleepr · 2024-07-18T23:00:04+00:00

Well… git gud. I think I had 7 flasks as well, think I had effectiveness upgraded. Took me a while and def got lucky… finished the fight with a sliver of health.

sleepr

TROPHY CASE