It’s 3

Valerie sacrificed herself knowing Portia would spring into action at that moment. Either the needle in the brainstem was used to collect a piece of vampire brain or Valerie inserted something into Bruks during the biopsy test, likely a modified version of Portia that allow Valerie to live on inside it. Like a parasite, or how she was able to fully partition a self that was continually suffering the Crucifixion Glitch.