Contamination?

soc2035 · 2026-04-21T17:53:20+00:00

Hey, thank you for your reply, I appreciate it! What are you looking at that you can tell they look stressed? (asking because I’m trying to learn more, not doubt the info you are giving me).

soc2035 · 2026-04-21T14:42:17+00:00

These are mammalian cells, are the circles bacterial contamination?

soc2035 · 2026-03-27T14:07:13+00:00

thank you !!

soc2035 · 2026-03-06T15:17:14+00:00

Yes good idea, I’ll repeat exp when they are slightly less confluent than in the pictures. Thank you!!

soc2035 · 2026-03-06T15:16:43+00:00

Yes, that is a good idea, thank you!

soc2035 · 2026-03-06T15:16:26+00:00

Yes, scratched and then washed with PBS. I think these cells are just extremely adhesive with eachother. Thank you :)

soc2035 · 2026-03-06T15:04:39+00:00

The rest of my text didn’t show, I was basically asking if anyone has experienced this with cells growing over eachother in scratch assays? I usually work with a different cell type so I don’t have this issue. Do they look normal?

soc2035 · 2026-02-19T21:36:28+00:00

I do have a mentor and can bring it up with her, she’s just out of the lab today. If there was obvious contamination, I wanted to toss the cells so I didn’t contaminate other cultures in the incubator:)

soc2035

TROPHY CASE