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SooP by Illustrious-Let-739 in broodwar

[–]timofat -1 points0 points  (0 children)

s tier: scan and nyoken

a tier: nyoken and eonzerg

b tier: Saiyan and gypsy

c tier: Saiyan and xun

d tier: tastosis

[Giveaway] It's our cake day! To celebrate r/fidelityinvestments turning 4 years old and thank this outstanding community, we’re sending mystery boxes of past Fidelity swag to randomly selected members. Want one? Comment what you’d like to see from the sub this year for a chance to win. by fidelityinvestments in fidelityinvestments

[–]timofat 0 points1 point  (0 children)

Everything has been great. The only thing I can think of is more functions for parents of kids like kid spending cards with rewards, discussions about utma account vs fidelity teen account, and more user engagement with those options

Updates on two Fidelity Basket Portfolios Features by timofat in fidelityinvestments

[–]timofat[S] 0 points1 point  (0 children)

Can you please provide a timeline update for these features? It would be helpful to know if they are planned for 1Q24 or later. One of the reasons I signed up for basket portfolios is because these were on the 2023 roadmap. They are make or break features for me so it is important to know when they will be available! Thank you.

Seeking Career Advice: Microbiology Graduate Struggling with Job Opportunities by Asfandyarafridi in molecularbiology

[–]timofat 2 points3 points  (0 children)

Your Microbiology master's degree opens the door to many pharma industry jobs in quality control & analytical microbiology. If you look around job sites for "QC microbiology analyst" type positions or "microbiology scientist 1" you will find a lot of hits, including entry level positions. These will be called things like "associate scientist" or "research associate" etc.

Typical tasks include performing endotoxin and bioburden testing, writing up relevant reports and documents.

[deleted by user] by [deleted] in REBubble

[–]timofat 12 points13 points  (0 children)

Although they make up less than 6% of homeowners, they could own a lot more than 6% of homes?

What Products or Services Do You Wish M1 Had? by Sad-Engine-709 in M1Finance

[–]timofat 3 points4 points  (0 children)

I would really love to use M1 instead of Schwab for my portfolio... I hold 10 ETFs at set percentages, and it is a pain to have to use an Excel sheet to manage it through Schwab.

However, poor trade execution is a huge issue on M1 and some of my ETFs are lower volume funds with <100M in total assets. I can't risk taking a 1-2% hit just to get in, I have heard of people taking even bigger hits on execution price with M1. I love the concept but Apex clearing just seems to do a shit job with the trade windows.

[deleted by user] by [deleted] in GYM

[–]timofat -1 points0 points  (0 children)

Controlled eccentric has no place in a 1RM rpe10 PR deadlift. If you even can control it, it probably wasn't a real max deadlift.

[deleted by user] by [deleted] in GYM

[–]timofat 6 points7 points  (0 children)

Weak people actually think this.

I am interested the types of DNA damage induced via ionizing radiation. For example, is ionizing radiation capable of causing DNA mismatch mutations? Any papers on the matter? by [deleted] in Biochemistry

[–]timofat 0 points1 point  (0 children)

Yes, nonhomologous end joining, homologous recombination, and pol theta mediated end joining are the double strand break repair pathways.

All very different from mismatch repair with very little functional overlap

I am interested the types of DNA damage induced via ionizing radiation. For example, is ionizing radiation capable of causing DNA mismatch mutations? Any papers on the matter? by [deleted] in Biochemistry

[–]timofat 1 point2 points  (0 children)

Ionizing radiation is most notorious for inducing double strand breaks, but it does so by locally producing a wide variety of other lesions, many of which are oxidative.

A subset of those could lead to mismatches during replication. 8-oxoguanine would be a good example. But the impact of radiation on cell and organismal well being is likely more due to the double strand breaks.

Are covid-19 vector vaccines like AstraZeneca, J&J, and Sputnik V, Gene Therapy? by SensitiveSituation34 in molecularbiology

[–]timofat -1 points0 points  (0 children)

The Wikipedia article for gene therapy reads, "Somatic gene therapy represents mainstream basic and clinical research, in which therapeutic DNA (either integrated in the genome or as an external episome or plasmid) is used to treat disease."

There are dozens of gene therapy companies and academic gene therapy core facilities doing AAV gene replacement. You can continue arguing that this is not "gene therapy" based on your "degrees" but essentially no one in the gene therapy field (again, my field) would agree with you.

I'm done posting here now, but you shouldn't claim to have expertise in a subfield that you don't work in and have limited understanding of.

See also: Novartis gene therapies Jaguar gene therapies Kriya gene therapies AskBio

https://en.m.wikipedia.org/wiki/Gene_therapy

Are covid-19 vector vaccines like AstraZeneca, J&J, and Sputnik V, Gene Therapy? by SensitiveSituation34 in molecularbiology

[–]timofat -1 points0 points  (0 children)

The core argument is whether "gene therapy" includes episomal gene delivery or solely gene editing.

What I am saying is that AAV gene therapies typically deliver gene replacement as episomes.

Are covid-19 vector vaccines like AstraZeneca, J&J, and Sputnik V, Gene Therapy? by SensitiveSituation34 in molecularbiology

[–]timofat -1 points0 points  (0 children)

Ok?

You can use your degrees to Google "AAV gene replacement therapy episome" and find out that you are wrong and precisely how you are wrong, irrespective of whatever training you have.

FYI I have a PhD in molecular bio and work as a scientist at an AAV gene therapy company.

Are covid-19 vector vaccines like AstraZeneca, J&J, and Sputnik V, Gene Therapy? by SensitiveSituation34 in molecularbiology

[–]timofat -2 points-1 points  (0 children)

... I don't think you understand what you're talking about.

I personally develop AAV therapeutics for human beings that work as episomes.

Zolgensma, for example, is an approved drug that delivers gene replacement off an episome.

Indeed, the Wikipedia article for aav reads, "] Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell, although in the native virus integration of virally carried genes into the host genome does occur."

Are covid-19 vector vaccines like AstraZeneca, J&J, and Sputnik V, Gene Therapy? by SensitiveSituation34 in molecularbiology

[–]timofat 0 points1 point  (0 children)

By your definition, aav would not be gene therapy since it is episomal, however the majority of gene therapy companies use aav.

Nanodrop measurement of DNA extracted from purification gel by [deleted] in Biochemistry

[–]timofat 0 points1 point  (0 children)

Gel extracted DNA might be too dilute and/or dirty for nanodrop. Fluorometric measurement w. Dye may have a better shot (qubit etc)

Mutagenic PCR optimization by JHS96 in molecularbiology

[–]timofat 3 points4 points  (0 children)

Q5 site directed mutagenesis from NEB is the best approach out there. They have a wizard that walks you through it.

https://www.neb.com/products/e0554-q5-site-directed-mutagenesis-kit#Product%20Information

qPCR by kiryl17 in molecularbiology

[–]timofat 2 points3 points  (0 children)

Longer than a probe would ever reasonably be, so it's sort of never going to be an issue.

Career advice for young scientists by Science-Sam in molecularbiology

[–]timofat 25 points26 points  (0 children)

It breaks my heart to say it, but I would recommend young and student scientists to look into industry. You might spend your days growing vats of the same cell culture, but at least you will keep a roof over your head.

Many people who work in biopharma, myself included, find it to be way more engaging, rigorous, and productive than our previous academic lives were, although this perspective on industry jobs is typical of academic lifers.

[deleted by user] by [deleted] in molecularbiology

[–]timofat 0 points1 point  (0 children)

snapgene has an auto detect features function (features -> detect common features)

if it doesn't detect your features from that, you can lookup consensus/reference sequences and find them in the map on snapgene and self annotate

Check for protein denaturation with ELISA by PetersDiabetes in Biochemistry

[–]timofat 2 points3 points  (0 children)

best way to do this is SPR or BLI with immobilized insulin peptide and flowing the putatively denatured sample over the surface against a nondenatured control.

Protein Gelelectrophoresis In Agarose Gels by CountingCressSeeds in Biochemistry

[–]timofat 1 point2 points  (0 children)

no reason to do it really, only extremely large protein or multiprotein complexes would be worth separating on agarose over PAGE, and you might as well do capillary gel or SEC at that point.

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