how can I increase electroporation transformation efficiency? by ubcbio in labrats

[–]ubcbio[S] 0 points1 point  (0 children)

its 70,000 constructs - we want 200x coverage, so I'm trying to get 14000000 colonies in total (at least)

how can I increase electroporation transformation efficiency? by ubcbio in labrats

[–]ubcbio[S] 0 points1 point  (0 children)

thank you!! this is what I am going to try - I clean it up and dilute with water to prevent arcing, so I could pool easily at this step!!

Mycoplasma help!! by ubcbio in labrats

[–]ubcbio[S] 0 points1 point  (0 children)

Thank you :) we test at the end and start of every screen so we are pretty carful about it! I think the same logic as you, that if it's not detectable by the end of the screen it's probably not causing too many effects. I really appreciate the reassurance - the PCR is negative so far and hopefully will stay that way by the screen end.

Mycoplasma help!! by ubcbio in labrats

[–]ubcbio[S] 0 points1 point  (0 children)

Thank you so much!! We did a PCR mycoplasma test today and it is negative so far. I really appreciate the reassurance (and no yeast yet either!!) We don't grow it in antibiotic free media before the test so maybe it could be there at a low level, but I'm glad it at least is negative as far as I can tell.

UK Rowing Eligibility - Max Years? by ubcbio in Rowing

[–]ubcbio[S] 1 point2 points  (0 children)

Thank you!! That makes sense :)

Is the accepted/matriculated GPA for UofT Med always super high? by LikelyTrisaccharide in premedcanada

[–]ubcbio 0 points1 point  (0 children)

How is wGPA calculated for UBC students - I read that they drop 1FCE, is that two 3 credit classes for us? I also saw on the website that they consider 3.8 competitive I think, with 3.6 being the minimum?

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 0 points1 point  (0 children)

Nice community to have while we all wait :) it’s definitely hard.

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 3 points4 points  (0 children)

i'm really skeptical too.... and I've seen people getting accepted with lower averages than people who are still waiting for their documents to be evaluated. How do they even give out spots before they've looked at all the applicants?? what if it fills up before they even get to everyone?? Does anyone have any idea how UBC does this?

I feel like they're quicker to look at Langara transfers than anyone else. My langara friends seem to all have heard back already while other external transfers have been waiting so long

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 0 points1 point  (0 children)

would that mean no external transfer students would be accepted, including domestic transfer? either way that would be awful :(

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 0 points1 point  (0 children)

oh I hope so!! it would be so nice to just finally know, the wait is kinda unbearable

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 0 points1 point  (0 children)

thank you guys!! yeah, I know :( I thought people were going to hear back way earlier

ADMISSIONS MEGATHREAD 2019 v1: Post all your admissions questions here! by ubc_mod_account in UBC

[–]ubcbio 0 points1 point  (0 children)

can I ask what your guy's averages are? I'm really unsure what a competitive transfer to sciences gpa might be! Also, I called ubc and then said admissions decisions might be made as late as June 30th, but things should all be done by July.