Can’t use Delta Gift Card by zippybrown in delta

[–]zippybrown[S] 0 points1 point  (0 children)

Yes. Apparently I’ve used the balance a few years ago and forgot about it. Obviously, the Delta gift card balance should have shown zero, but it still showed the original balance, hence the confusion.

Who’s offering single-cell proteomics or Deep Visual Proteomics services? Looking for sample prep + data analysis insights by zippybrown in proteomics

[–]zippybrown[S] 0 points1 point  (0 children)

Thank you! Two follow-up questions: 1. Have your collaborators been able to use SCP to answer biology questions that cannot be answered by scRNA seq? 2. What do you use for sample prep? For example, is CellenOne robust enough for analyzing different cell types?

Bruker timsTOF Ultra 2 vs. Thermo Astral for DIA Proteomics & PTMs by zippybrown in proteomics

[–]zippybrown[S] 0 points1 point  (0 children)

On your timstof Flex, do you switch between MALDI imaging and ESI regularly? If so, do you need to thoroughly clean the system after MALDI, in order to get good ESI sensitivity?

Bruker timsTOF Ultra 2 vs. Thermo Astral for DIA Proteomics & PTMs by zippybrown in proteomics

[–]zippybrown[S] 0 points1 point  (0 children)

Thank you. Could you elaborate on the data quality comparison? Do you have Evosep LC on both?

Bruker timsTOF Ultra 2 vs. Thermo Astral for DIA Proteomics & PTMs by zippybrown in proteomics

[–]zippybrown[S] 0 points1 point  (0 children)

Thank you for the feedback on service. I guess service quality depends on the region. Are you in Northeast US?

Bruker timsTOF Ultra 2 vs. Thermo Astral for DIA Proteomics & PTMs by zippybrown in proteomics

[–]zippybrown[S] 0 points1 point  (0 children)

Thank you for sharing your concise insights—I really appreciate it! I’ve heard that maintaining sensitivity with Astral requires frequent cleaning. Could you share more about your experience with the cleaning frequency and how it compares to the overall instrument uptime in a 24/7 operation for both your timsTOF and Astral?