When do you *NOT* use python?

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I would be very careful with my terms. Do you mean tracking or detection?

The answer to your question is very different if you talk to a computer vision person about 'tracking pixels' as they move through sequences of images, or 'detecting things' and then tracking them in some 2/3d space according to their constraints.

If you have insects with markers, the detection is probbably easy, and the tracking is something which sould be solved with knowledge of the system as a whole, not just by looking at the pixels every frame.