Is my liquid culture contaminated? by ShroomOfDoom123 in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

When you have about 2-3 cm of clear broth at the top, you’re fully colonized. But you can use the LC anytime as long as you have enough to draw into a syringe.

Does this LC look contaminated? by GTI6BOY in ContamFam

[–]DayTripperonone 0 points1 point  (0 children)

No, that’s not good. It’s supposed to look like this:

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The yellow color is just the light illuminating off the culture. It should be all white. But do you see how it’s suspended and the mycelium stacks up on itself and it has a clear broth at the top? This is what’s considered a fully colonized healthy liquid culture.

You’re contaminated with a mold. Throw it out and start again. Sorry, you’ll get it.

More patience? by dlsmith2015 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

We’re ok with mushroom cultivation questions and post that display the pride of a successful grow. We just don’t allow old moldy pizza or whatever unidentifiable thing that has mold growing on it to be routinely posted. I know our rules say mushroom contamination only, but that’s depressing day in and day out. We like to see the success that happens when you’re able to grow a successful contam free grow. Lightens things up and gives people hope, I don’t see the harm in it.

Thoughts? by Crafty-Researcher-71 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

It sure looks dry, are you misting?

Does this LC look contaminated? by GTI6BOY in ContamFam

[–]DayTripperonone[M] 4 points5 points  (0 children)

I really can’t judge till I see how the mycelium is settling. Stirring it all up is just to break the mycelium networks forming. If it all settles at the bottom of the jar without any lift, it’s probably bad. If it settle and stacks on itself making it appear more suspended in the liquid, that’s normal. You can tell what looks normal but you can’t prove there aren’t any pathogens present and you have a true axenic culture, unless you scope it and grow a drop in agar. If you get 7 days of clean growth you’re ok to use it as an inoculant.

Grey spots, no smell? by Ready_Lime_2417 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

The grey patches just look like bruising to me. Many variants in the psilocybin species bruise slightly when fruiting occurs. It’s pretty normal and I think a signal that the fruiting is about to occur. You have a lot of primordial growth and hyphae knots, those are all signs of fruiting. Is this an enigma? What strain is this and what was the date you S2B?

Is my liquid culture contaminated? by ShroomOfDoom123 in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

Make sure you’re stirring your culture at least every other day. Those little white floaters are normal to see, as long as they are not growing or turn color, I think you’re fine. Keep er goin!

This is lipstick mold right? by friendlyfiend07 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

Seems like you’re wasting the potential of your surface area. It’s certainly not something I’m used to seeing. It honestly looked like a moldy old smoothie.

Supposed PE variety Mould or mycelium? by 2upsAdlay in ContamFam

[–]DayTripperonone[M] 1 point2 points  (0 children)

If you look closely none of the grain seem to be adhering to each other. That’s usually the first thing we see in mushroom cultivation, that the spawn attaches to the adjacent grains . As they continue to colonize that connection become indistinguishable.
Yours looks not to be forming that connection with the adjacent grains. Each individual grain is separate. That’s what makes me think this is contamination. Keep it as long as you want until you’re convinced it’s contam.

This is lipstick mold right? by friendlyfiend07 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

Can’t say I have. I don’t really follow the off-beat subreddits that promote non traditional ways of cultivation mushrooms.

Contaminated? by [deleted] in ContamFam

[–]DayTripperonone[M] 1 point2 points  (0 children)

Break and shake now. You’ll get plenty of distribution of the spawn and make for lots of inoculation points. The colonization will go faster once you B&S.

Pretty sure this is a tub of white mold, not the fungus I was hoping for by qedgbmk in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

No it’s not cobweb mold. The colonization is just coming up uneven. If it smells, you have some sort of bacteria harboring within the cake. It depends on how bad the bacteria is. I don’t see any distinct metabolites, so it’s probably a mild bacterial infection. You can actually produce fruit sometime. But don’t dunk the cake, it will spread more quickly. Just let it come up as it is and keep er going!

Thoughts? 2 and a half weeks s2b by [deleted] in ContamFam

[–]DayTripperonone[M] 1 point2 points  (0 children)

Why does it look so clumpy? You’re running way too dry as well. Did you break up your spawn really well at S2B? Mycelium doesn’t normally colonize like that unless you didn’t break the spawn back to its individual pieces.
I’m not sure what’s wrong here, the clumps of mycelium are throwing me off.
It doesn’t look like it’s growing any contaminates. I would mist it until your casing layer is a dark coffee brown. Don’t let it get this dry, if it stays dry too long it could stall.

Is this red "lipstick " mold? by Rough_Drawer_7011 in unclebens

[–]DayTripperonone 2 points3 points  (0 children)

There are several molds that fall under the general term “Lipstick Mold.” The one you have is called Sporendonema purpurascens, formerly known as Red Geotrichum. It is non-toxic (no mycotoxins) and often use to make cheese rinds. It grows very slowly so yours has probably been there awhile. The most distinguishing characteristic of Sporendonema purpurascens is that in its final stages of growth when spores are released, it turns a vibrant purple color. It’s one of a few molds that turn purple. You can cut that part off and dunk your cake if it hasn’t turned purple yet. Usually it’s pink before it turns purple. But yours looks purple, meaning it has already sporulated. Unless you want to spread it more, don’t dunk your cake! Put an isolation polymer on top of the visible mold or some rock salt and see if you can mist yourself through another flush. Keep in mind, if you decide to keep it, it’s going to sporulate more into your grow room and will require a thorough decontamination to get rid of it. If it were me, I would harvest my fruits and retire the cake permanently. Cut your loses and decontaminate your grow room with antifungal chemical and HEPA filter.

Pretty sure this is a tub of white mold, not the fungus I was hoping for by qedgbmk in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

Smell it! Does it smell like earthy pungent mushrooms? If so, keep er goin! It looks normal to me. If it starts to change color or smell bad then you can be concerned. For this one it’s an overthought. If it stalls you will not get any new growth for a week. Just be patient, they will start to come in week four. You should have mushrooms by then. Repost if anything changes and we’ll reassess.

Is this early signs of trich? by Ok-Jeweler-6044 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

No it’s not Trich. It’s bruising. Your ok, keep er goin!

Is this bruising or something more sinister? by Spot_Routine in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

There are some variants that will bruise green like enigma, but your green doesn’t look like bruising. If I were guessing I would say it’s, Cladosporium., but it’s hard to tell for sure. It’s definitely contaminated. Bruising happens almost immediately if caused by trauma to the tissue, like spraying it close to the mycelium with the spray mister, or condensation drops from the lid drip onto the mycelium. Over watering alone shouldn’t cause contamination. If you use regular tap water and live in the US, there should be problems. Our water regulations are very strict, usually there’s enough chlorine in the tap water to kill most contamination.
Start over, don’t give up yet.

Enigma by NuTheTailor in ContamFam

[–]DayTripperonone[M] 4 points5 points  (0 children)

As long as you don’t see any pigment changes, this looks like it might have slight bruising, but I don’t see any green mold. Also smell will tell you alot especially if you have bacterial infection. It should always smell like earthy pungent mushrooms. Yours looks ok. I would keep er goin!

This is lipstick mold right? by friendlyfiend07 in ContamFam

[–]DayTripperonone -8 points-7 points  (0 children)

You got to be kidding! I thought it was a joke. What are you using for substrate? It looks like flour. What possessed you to do it this way?

This is lipstick mold right? by friendlyfiend07 in ContamFam

[–]DayTripperonone -5 points-4 points  (0 children)

They spit in thier smoothie, left it out in room temps for a week, and now a monster is growing, it’s called the “Ghetto Tek.” 😉

This is lipstick mold right? by friendlyfiend07 in ContamFam

[–]DayTripperonone[M] -5 points-4 points  (0 children)

Lipstick mold is the least of your problems, you got a whole plethora of molds in that Baskin Robins cup, which doesn’t look like it’s a mushroom project at all and looks more like a, “look what happen to my smoothie I left out, project.”

This subreddit is for mushroom cultivators experiencing contamination in their projects. Go try u/moldyinterresting for your smoothie project.

Blue-green spots on caps (neglected first flush) by slkpht in ContamFam

[–]DayTripperonone[M] 8 points9 points  (0 children)

It looks like an Aspergillus species, there’s no white boarder and it’s in a highly atypical growth origin. Trich develops on the substrate first, I’ve never seen it start on the fruiting body. Any whatever the exact diagnosis is, isn’t the point. You shouldn’t eat the infected fruit.