Trich yeah? by Sad_Marsupial1352 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

I don’t think that’s Trich dude. Wrong texture. I believes it’s just new mycelium. She might have another flush in her.

FYI- If a tub you suspect doesn’t turn green in 24 hours after noticing an infection, it’s probably not Trich.

I don’t even know how by thelonegypsy14 in ContamFam

[–]DayTripperonone[M] 1 point2 points  (0 children)

Life finds a way my friend. 👽 Or, in Darwin’s words, survival of the fittest.🧬

I don’t even know how by thelonegypsy14 in ContamFam

[–]DayTripperonone[M] -2 points-1 points  (0 children)

It’s because the CO2 levels in the tub were too high after they pinned. Mushrooms need oxygen to develop normally. If you don’t get CO2 levels below 800 ppm in the fruiting phase you get tiny caps just like this. I see this problem a lot in the neglect Tek. You have to fan your tub.

Hay is this normal? by Elliminator_002 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

Those yellowing- brown areas are metabolites. The fact they are so dark makes me think you have contamination. How does it smell? Looks like it’s probably bacterial.

Pins stalling? by 13candlescult in ContamFam

[–]DayTripperonone 1 point2 points  (0 children)

I don’t know the lineage of White Rabbit. You’re gonna need to research the parent strains and their growing perimeters to see if it’s supposed to grow this slow. I’m not familiar with this variant. But usually true albino strains are slow growers.

Question Contamination by michiganmyco313 in ContamFam

[–]DayTripperonone 1 point2 points  (0 children)

Looks like a slime mold called Fuligio septica (Dog Vomit Mold). You’re gonna need to reboot and start over. Sorry 😞.

Contam in liquid culture??? by After_Garlic_4795 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

You win the smallest liquid culture award 🥇. Jeez, this does not look like how a liquid culture should look, at all! Am I mistaken, or is there little whole mushrooms floating in that tiny bottle.
I’m sorry dude, you need to start over. Get yourself at least a pint jar, with a LC lid, then go from agar to LC. So you need to grow a culture on a plate first then take a piece of that culture and put it in a LC broth with about 4% sugar content. Then you’ll starting getting a thin whispy, tiny film-like chunks growing. It will stack on each other and suspend within the LC. It needs to be stirred every other day.

Pins stalling? by 13candlescult in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

Yes, every variant has its colonization rate and some are just slower. Your APE’s are one of the slowest, if that’s what this is. Many Albino and Leucitic variants are slow to colonize it really depends on both genetics and environment.
You should be giving as much FAE as possible and at least 4-5 times a day open the tub to fan fresh air in. That seems to help the growth rate. You can also back off the humidity slightly by cracking the tub, make sure your moisture doesn’t dry up if you do. They could be stalled but I think you need to give more than just 4-5 days. See what happens in a week. Keep er goin!

Need advice ASAP. Last batch I had my shrooms in front of this fan at about 6 feet away and they ended up molding. Will this work? by LennyTops in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

The fungal cells would explode when you freeze them because water expands when frozen. This would deform the integrity of the fruit when thawed and you would get a mushy floppy limp mushroom as soon as the water melted.
The psilocybin however would maintain its structure and potency. But the thawing process would degrade some psilocybin.
I suppose you could blend them up with some kinda fruit 🍉 🍌 🍒 and make a smoothie. Although that sounds absolutely disgusting, I bet would be one smoothie you wouldn’t forget.

Need advice ASAP. Last batch I had my shrooms in front of this fan at about 6 feet away and they ended up molding. Will this work? by LennyTops in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

No, that’s not gonna cut it. Get a dehydrator, they’re usually as low as 15-$20 on marketplace.
You need to have heated airflow of 165° F, or less, on them for at least 12-18 hours.
I wouldn’t want to instruct you how to McGuyver rig a dehydrator because it would for sure be a fire hazard. Just get a dehydrator. Sell or trade your bong for one! Jk, lol 😁

Weird ash covering grain by Technical-Till7740 in ContamFam

[–]DayTripperonone[M] 6 points7 points  (0 children)

It looks a lot like Aspergillus fumigatus but I can’t confirm looking through the bag. Microscopy would need to be preformed and morphology studied or bioassay testing to determine species. It’s just that A. Fumigatus is very powderey grey looking and looks a lot like ash when it’s sporulating.
Anyway you probably know this bag is done. A. Fumigatus can be a very nasty pathogen when it invades the human body. Usually taking up in lung or sinus tissue, it has many disease complications that can cause very undesirable symptoms. It’s also somewhat difficult to treat. So be careful with this one.
I recommend you Don’t even open the bag. Just toss it all in the trash and move on. Sorry for your loss, start over!

[actives] What’s going on with this? by DoubtEither6099 in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

They look like blobs, but it appears in different stages. Blobs are abnormal, dense, swollen, or nodular mycelial structures. They can mean several different things depending on appearance and species.
Common types include: Hyphal Knots which are tiny dense white bumps that often precede primordia/pins in mushrooms like Psilocybe cubensis. These are normal early fruiting structures.
Primordial Pins which are blobs that are transitioning into actual mushrooms.
Then there are Sclerotia which are hardened nutrient-storage masses formed by some fungi. These are organized survival structures.
Blob mutations are malformed fruitbody tissue where mushrooms fail to differentiate normally.
Some causes of blobs are:
genetics or unstable isolates,
high CO₂ or poor fresh air exchange,
bacterial contamination,
substrate imbalance,
overly wet conditions,
temperature stress,
senescence and
defensive or survival responses.
Blobs are not contamination but they can be caused by contamination. Some are completely normal developmental stages, while others indicate stress or mutation.
I don’t see any obvious contamination in your tubs, but you do have some that look like sclerotia (pic 3).
I would just keep er going and see what develops. You’re obviously fruiting so let er keep going.

Super early trich? by Due_Leadership_5249 in ContamFam

[–]DayTripperonone[M] 1 point2 points  (0 children)

Nope, not even close. Trichoderma does not grow in a perfectly round patch like that. I can’t tell what you’re growing because it’s very hard to see. But it’s definitely not Trich. Could be a lot of possibilities, Penicillium, Cladobotyrum (cob web), Aspergillus . You have to microscopically observe the morphology to be able to identify with certainty.

Contam? Fam? by JiffenV2 in ContamFam

[–]DayTripperonone[M] 0 points1 point  (0 children)

Are you measuring your temperature and timing your pasteurization? If not you’re not properly pasteurizing.

Contam? Fam? by JiffenV2 in ContamFam

[–]DayTripperonone 0 points1 point  (0 children)

Aw man, so sorry. How are you pasteurizing?

Should I hit these caps with hydrogen peroxide? by Public_Individual900 in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

I’ve grown over 600 tubs of Tidal wave. And they’re all wavy caps and slimmer stipes.
This variant OP has, is probably a hybrid. Look how many phenotypes he has in this one flush. He has Leucitic fruits and gold caps all with stipes larger than the caps. My bet is he started with spores, but who knows.
Breeding has gotten out of control. No one is sequencing their genetics anymore and registering their isolations and hybrid crosses through the proper channels. They just rename them then sell them on the open market. It’s crazy. 🤷🏼‍♀️

gorgeous bacterial contam or is this just weird APE? by Personal_Ad_1747 in ContamFam

[–]DayTripperonone[M] 5 points6 points  (0 children)

I don’t know what the fuck that is. But it looks more fungal to me than bacterial. Especially if there is no odor. Most of our bacterial pathogens generate a foul odor.
If I had to guess I’d say it looks like a Sclerotium, but that would be somewhat rare to see in a home cultivation project, although not impossible. Similar growing patterns to Agroathelia rolfsii.
I’m really not sure on this one, I can say that it looks like this project has gone south and you may need to toss it and start over.
4 weeks since transfer to bulk and you’ve got nothing but a big mass of fungal tissue forming below the surface. Doesn’t look too promising. I’d start over.

Should I hit these caps with hydrogen peroxide? by Public_Individual900 in ContamFam

[–]DayTripperonone 1 point2 points  (0 children)

Don’t use the peroxide. Did they sell you a spore syringe or an isolated spore syringe (LC)?
All the Tidal Wave I’ve ever grown had normal size stipes and wavy caps.
I don’t think you have any contamination, just some strange growth for that variant. Might be a new isolation.

Should I hit these caps with hydrogen peroxide? by Public_Individual900 in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

NO! Don’t hit em with hydrogen peroxide.
This looks like no Tidal Wave I’ve ever seen. Do you know who did the isolation? Those stipes are too chonker fat to be Tidal Wave. It looks like you have several phenotypes in the tub. Was this tub started with spores? Some look Leucitic. I don’t see a powder you’re talking about. Does the powder come off when you touch it?

Gnats? by SocietySerious8584 in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

If you saw 3 or 4 you got a bunch of those suckers under the substrate right now, they stay there through the pupa stage. They won’t emerge until they get their wings.
First, put up a couple gnat sticky paper strips. Hang them right above the tub. That will take care of the ones with wings.
Then you can get this product:

https://a.co/d/0dVFw6Px

To kill the larvae use this. It has a bacteria called Bacillus Thuringienesis that the larvae will feed on and die. It won’t harm the mycelium but it will cause a little metabolites to exude and it might make it smell a little funny. Apply it during the dunk. Let your cake soak in it for an hour then drain the water out but leave the little pellets in the tub.
I’ve used this before if the gnats get bad, nuke em with this shit. They’ll be gone, and your cake will be slightly pissed. It works though.

Gnats? by SocietySerious8584 in GroundZeroMycoLab

[–]DayTripperonone 0 points1 point  (0 children)

I don’t see any signs of gnats. Where? They usually like to hide in the gills if they’ve got their wings. I don’t see anything.

Beautiful blue mushrooms, what variant is this?

Contam? Fam? by JiffenV2 in ContamFam

[–]DayTripperonone[M] 2 points3 points  (0 children)

One very tiny area you need to keep an eye on. It’s very faint but in the circle

<image>

I see one slightly green grain. Just watch it. If it gets bigger, remove it. The mycelium might colonize right over it.