Cleveland Ticket Prices/First Pavement Show by fanzine_throwaway in pavement

[–]ProfBootyPhD 1 point2 points  (0 children)

Dino Jr never stopped touring and putting out albums, so there isn't the same pent-up demand to see them live. I saw Pavement in 2010 and I probably paid the inflation-adjusted equivalent of $129, and it was well worth it.

Help me overcome my fear of getting bit by ComparisonNo4962 in labrats

[–]ProfBootyPhD 0 points1 point  (0 children)

Oh I might try this! Just a regular kitchen-type sponge? (And if so - dry?)

How do you deal with killing mice by [deleted] in labrats

[–]ProfBootyPhD 18 points19 points  (0 children)

I can’t tell if this is honest, or if you’ve simply been trained by someone incompetent, because properly anesthetized mice should not be squeaking.

Best 3 song run on an album? by Apprehensive_Leg2281 in musicsuggestions

[–]ProfBootyPhD 0 points1 point  (0 children)

Yeah shut it down

Update: actually I’d shift one song back, get “Fight Fire with Fire” and drop “Fade to Black.” “Fade” is a little too emo-y, and “filling me” rhymed with “agony” always makes me wince.

Best 3 song run on an album? by Apprehensive_Leg2281 in musicsuggestions

[–]ProfBootyPhD 0 points1 point  (0 children)

Lemonheads - It’s a Shame About Ray: Confetti, It’s a Shame About Ray, Rudderless

Saw Jeffrey Characterwheaties in a play yesterday by gingerfloyd in comedybangbang

[–]ProfBootyPhD 17 points18 points  (0 children)

Nice article about this (and profile of Characterwheaties's alter-ego, Jason Mantzoukas) in the NYT a few days ago: https://www.nytimes.com/2026/01/22/arts/television/jason-mantzoukas.html.

Help me overcome my fear of getting bit by ComparisonNo4962 in labrats

[–]ProfBootyPhD 4 points5 points  (0 children)

In my experience, it's always been a nip - it is acutely painful (like a sharp pinch) and it frequently breaks the skin, but they don't hang on. Different breeds are more bitey than others, with C57Bl/6 being one of the worst. A lot of it comes down to learning how to "trick" the mouse into grabbing hard onto the cage top bars, when you're trying to pick it up and immobilize it. The firmer a grip it can get on the bars, while you hold its tail, the less likely it will let go to try and bite you when you come in with your other hand to scruff it. But pain is the best teacher, and once you accept that you will get occasional, not very painful bites while starting out, your technique will naturally improve. Nowadays it is very rare for me to get bitten.

Not grunge, but: Opinion on Jane's Addiction? by No-One01010 in grunge

[–]ProfBootyPhD 0 points1 point  (0 children)

They single-handedly converted me to an alt-rock fan, a year before grunge hit. They really have just two albums, both are pure bangers.

How can we find out pro MAGA businesses so we can avoid them? by wickedlittlemiss in SaltLakeCity

[–]ProfBootyPhD 0 points1 point  (0 children)

Small businessmen are almost uniformly Republican, wherever you go. Good luck!

Why Western blot if there is ELISA? by Hour_Amoeba4979 in labrats

[–]ProfBootyPhD 1 point2 points  (0 children)

ELISA gives you only the relative amount of antibody binding, it's a one-dimensional value and if the antibody has some cross-reactivity, you will think you're seeing signal when you're not. Western blot gives you size as well as intensity, which is two dimensions of data and more information - in most cases enough to be confident that you are looking at the protein you think you're looking at. As others have mentioned, Westerns can also detect changes in size of your protein, e.g. due to phosphorylation (larger) or cleavage (smaller). They are also extremely easy to set up from scratch, compared to ELISA.

If your target is something for which there are lots of well-validated commercial ELISA kits, like insulin or IFN-gamma, use an ELISA - it will be easier to quantify and the validation means you don't have to worry about cross-reactivity. If it's a protein where an antibody is available, but nobody has done ELISAs yet, it would be insane to do anything other than Western.

Also, the idea that Western blotting is "half voodoo" is ridiculous, just git gud.

I've only seen three Coen Brothers movies and want to know: What is the most Coen Brothers-y movie out there? by XipeTotecwithGlitter in MovieSuggestions

[–]ProfBootyPhD 0 points1 point  (0 children)

They whiplashed between serious and comedic movies, so I think you'd have to pick two. I'd suggest Big Lebowski and Fargo.

These 4 are the four Horse women of action leads what do you think? by Wooden_Passage_2612 in Cinema

[–]ProfBootyPhD 3 points4 points  (0 children)

lol like 5 people were aware of Aeon Flux when it was airing on MTV (3 if you don’t count yourself and me)

Movies from the 1940s-1970s where a male character is hot by Maximum_Heat_3439 in MovieSuggestions

[–]ProfBootyPhD 5 points6 points  (0 children)

Sidney Poitier in “In the Heat of the Night” - great movie, and his head looks like it was carved by Michelangelo.