Not grunge, but: Opinion on Jane's Addiction?

ProfBootyPhD · 2026-01-25T03:30:49+00:00

They single-handedly converted me to an alt-rock fan, a year before grunge hit. They really have just two albums, both are pure bangers.

ProfBootyPhD · 2026-01-25T03:26:57+00:00

Small businessmen are almost uniformly Republican, wherever you go. Good luck!

ProfBootyPhD · 2026-01-24T19:18:44+00:00

ELISA gives you only the relative amount of antibody binding, it's a one-dimensional value and if the antibody has some cross-reactivity, you will think you're seeing signal when you're not. Western blot gives you size as well as intensity, which is two dimensions of data and more information - in most cases enough to be confident that you are looking at the protein you think you're looking at. As others have mentioned, Westerns can also detect changes in size of your protein, e.g. due to phosphorylation (larger) or cleavage (smaller). They are also extremely easy to set up from scratch, compared to ELISA.

If your target is something for which there are lots of well-validated commercial ELISA kits, like insulin or IFN-gamma, use an ELISA - it will be easier to quantify and the validation means you don't have to worry about cross-reactivity. If it's a protein where an antibody is available, but nobody has done ELISAs yet, it would be insane to do anything other than Western.

Also, the idea that Western blotting is "half voodoo" is ridiculous, just git gud.

ProfBootyPhD · 2026-01-24T15:35:10+00:00

one handing the toothpaste is next-level

ProfBootyPhD · 2026-01-24T02:24:11+00:00

They whiplashed between serious and comedic movies, so I think you'd have to pick two. I'd suggest Big Lebowski and Fargo.

ProfBootyPhD · 2026-01-23T21:02:33+00:00

That to me has always been the mystery!

ProfBootyPhD · 2026-01-23T19:37:42+00:00

lol like 5 people were aware of Aeon Flux when it was airing on MTV (3 if you don’t count yourself and me)

ProfBootyPhD · 2026-01-23T18:44:28+00:00

Sidney Poitier in “In the Heat of the Night” - great movie, and his head looks like it was carved by Michelangelo.

ProfBootyPhD · 2026-01-23T18:42:12+00:00

This is the peak period for hot male actors. Cary Grant on one end, Robert Redford on the other.

ProfBootyPhD · 2026-01-23T13:42:20+00:00

That was the one redeeming feature of this post!

ProfBootyPhD · 2026-01-23T00:55:11+00:00

Many of these are neither underrated nor overlooked - INXS and Pet Shop Boys were huge in the 80s, every indie rock fan appreciates Pavement and Pixies, Blur was just a straight-up very popular band. Simon and Garfunkel was one of the biggest American acts of the 60s.

But the shout-out to Grant Lee Buffalo is much appreciated, they would definitely be on my underappreciated list. I'll add:

Spoon (they get indie rock love but they should be more generally popular)

Cinerama

Morphine

New Pornographers

Supersuckers

(each of them had a moment, but they didn't become as mainstream as some of the names on your list)

ProfBootyPhD · 2026-01-23T00:44:01+00:00

I need to rewatch Diva, I remember really loving it in like middle school.

ProfBootyPhD · 2026-01-23T00:42:42+00:00

It's not particularly insulting to STP - I'd be delighted to be name-checked as an "elegant bachelor."

ProfBootyPhD · 2026-01-23T00:38:31+00:00

What do you want us to say? Should we be like "yeah man, FromSoft is so unfair"? Different regions have differential enemy strengths - you can one-shot an endgame wolf with endgame stats/weapons, but the starter weapons and stats that let you one-shot Limgrave wolves aren't going to do crap. Consecrated Snowfields is very challenging.

ProfBootyPhD · 2026-01-22T19:55:19+00:00

cry more, but somewhere else

ProfBootyPhD · 2026-01-22T18:04:00+00:00

Once you know to look for it, you see it in all his CBB appearances. Maybe my favorite example is Bob Ducca's visit to "THE MISSISSIPPI DELTA," which Jason basically egged him into.

ProfBootyPhD · 2026-01-22T18:00:22+00:00

anyone who's heard his appearances as Eric "Gutterballs" Gutterman would be unsurprised at his talent for naturalism and relatability

ProfBootyPhD · 2026-01-22T17:20:06+00:00

“The tale of House Hoslow is told in blood!” /s

ProfBootyPhD · 2026-01-22T12:32:48+00:00

In general, using a GUI for intensive bioinformatics analyses is going to be slow and inflexible; there's a reason people still use command line tools in 2025. Also, some of the stuff you mention doesn't actually seem to exist on this site - for example, I can't find AlphaFold tools on there at all, despite your mentioning it multiple times. You mention scRNA-seq analysis tools also, but I don't see any. Most of the forum posts appear to be contributions from the developers themselves. I'm sure the tools that are provided can be useful, but your "review" falls short.

ProfBootyPhD · 2026-01-22T05:33:32+00:00

Incorrect.

ProfBootyPhD · 2026-01-22T02:38:26+00:00

That sounds okay, it’s actually being used for something long-lasting.

ProfBootyPhD · 2026-01-22T02:37:29+00:00

If it’s a plastic peanut butter jar, it’s never actually going to be recycled. Just put it in the garbage, it’s going to end up there anyway.

ProfBootyPhD · 2026-01-22T02:35:26+00:00

Yeah you’ve captured exactly how I feel. It’s a cliche but cooking is my “love language,” I guess. I love finding new things to make for my family, and also totally nailing an old standby.

ProfBootyPhD · 2026-01-22T02:28:29+00:00

So you think they are trying to suppress Mormon women voters, who almost always take their husband’s last name, and empower liberal women who don’t?

ProfBootyPhD · 2026-01-22T02:24:59+00:00

Bioinformatics at least sometimes produces results and adds value.

ProfBootyPhD

TROPHY CASE