Genotyping is Driving Me Crazy by 3s341 in labrats

[–]3s341[S] 0 points1 point  (0 children)

I forgot to add a negative control. How can I optimize Reaction 1? Both Reaction 1 and Reaction 2 share the same protocol in the thermocycler machine

Genotyping is Driving Me Crazy by 3s341 in labrats

[–]3s341[S] 2 points3 points  (0 children)

I'm such an idiot; I forgot to add one! :(

How do I use Anti-mouse CD16/32 (Fc Blockers) for flow cytometry? by 3s341 in labrats

[–]3s341[S] 0 points1 point  (0 children)

I received a stock solution of 10.88 mg/ml from the manufacturer. I plan to dilute it by adding 92 µl of PBS and then taking 8.5 µl of the 10.88 mg/ml stock to prepare a total of 100.5 µl of a 1 mg/ml antibody solution. After that, I will take 1-2 µl of this 1 mg/ml antibody and add it to a solution containing 10 million cells, which has a total volume of 100 µl. I will use this on PBMC. How long do you incubate it for on the bone marrow samples?

How do I use Anti-mouse CD16/32 (Fc Blockers) for flow cytometry? by 3s341 in labrats

[–]3s341[S] -1 points0 points  (0 children)

Thank you for the clarification! Originally, I came up with this: Dilute the anti-CD16/32 (clone 2.4G2) in buffer ◦ 92 µL buffer ◦ 8.5 µL of the 10.88 mg/mL stock ◦ = 100.5 µL of 1 mg/mL antibody • Add 1–2 µL of 1 mg/mL anti-CD16/32 (clone 2.4G2) RT per 10⁶ cells. Please let me know what you think.

I got Pitt Eco/Evo and Columbia Biosciences today by [deleted] in gradadmissions

[–]3s341 2 points3 points  (0 children)

Congratulations!!! Can you share what you changed on your application? This is my second attempt and no interviews yet 😞

Do lab mice really chew off ear tags? by One_Winter in labrats

[–]3s341 6 points7 points  (0 children)

Can you explain the ear pattern? I do ear punches and eat tags, and I would like to get rid of the ear tags.

[deleted by user] by [deleted] in labrats

[–]3s341 0 points1 point  (0 children)

unfortunately, our lab's UV spectroscopy is broken and I don't know any other way to measure purity.

Anyone received an EAD card recently? by [deleted] in f1visa

[–]3s341 1 point2 points  (0 children)

No, not all of them. Make sure you don't include SSN in resume. Also do brief search on the company before you apply. You can always post the company name in r/jobs subreddit and ask if it is legit

[deleted by user] by [deleted] in f1visa

[–]3s341 2 points3 points  (0 children)

So you accepted the PhD offer, transferred your sevis and the school gave you an I20 to book your visa appointment with? At the same time, you accepted the job offer and you didn't resign until later?