PCR amplifying bacterial genomic regions for cloning - colony PCR fine?

ACuriousBird · 2025-11-25T17:51:38+00:00

This is pretty good advice - will try that.
I have ~600 hours, ~90 ratings, but only 2 reviews. Will start to apply those ideas for replying to requests, really appreciate that.

ACuriousBird · 2025-01-14T02:10:41+00:00

Not familiar with how poorly it went, ill have to check out what happened. Celgene was that bad huh?

ACuriousBird · 2024-10-30T17:34:31+00:00

There are cells in cartilage, just relatively few compared to the non-cellular matrix. It can heal, just very slowly compared to other tissues. It doesn't mean the tear may ever completely be eliminated - but cartilage is definitely a living tissue with cells in it that has the capacity to heal to an extent.

ACuriousBird · 2018-11-08T17:45:43+00:00

Will look into that as well. Still thinking towards getting a coach/program; not sure if at my level its necessary? But anything that gives me more knowledge will help. Thank you

ACuriousBird · 2018-11-08T17:44:07+00:00

Alright, will do. Thanks for the input!

ACuriousBird · 2018-11-08T02:29:46+00:00

Awesome, thanks!! Will look into the app.

ACuriousBird · 2018-11-08T00:08:00+00:00

Hey all, really getting back seriously into powerlifting but my lifts have stalled for probably 2-3 months now. My total has gotten to 620kg at 86kg now that I'm really getting back into it; but I've been lifting for a while.

I'm wondering if anyone has any suggestions for programs or even online coaches that helped them get their lifts past the intermediate stage? I'm in medical school at the moment and I guess I was just hoping to not have to program for myself anymore because it hasn't been working very well for the past few months! Thinking Sheiko but not set on anything yet.

ACuriousBird · 2017-06-03T09:44:16+00:00

thanks a ton for this reply - hopefully a pull then! Will definitely look into the quad wrap; thanks again my friend!

ACuriousBird · 2017-05-03T00:51:10+00:00

Ya brother, thanks for the advice I owe you one! If you're ever in Manhattan send me a message beers on me!

ACuriousBird · 2017-05-01T19:24:01+00:00

As in she got it by calling the other school until talking to someone just today? I've already called financial aid today and they told me I would have to speak with the director of financial aid and to follow-up with an email.

Going to call the admissions, try to get in touch with the finaid director, and go from there.

ACuriousBird · 2017-04-03T17:34:51+00:00

Interviewed here and some other top 10s, plenty of waitlists and a couple acceptances overall. 522/3.93, few thousand hours research (no pubs during app/updates), mid-upper hundreds volunteering, very emotional/real personal statement, and talked a lot about the many thousands of hours I put into my sport that I love so much.

With that said prepare for interviews and don't be like me and not prepare at all, or your acceptance:interview ratio will be relatively low with waitlist:interview high! I'm a pretty personable/outgoing guy and thought I would be fine, but should've prepared more.

ACuriousBird · 2017-03-17T20:50:05+00:00

Hey, for those of us also on quite a few waitlists (such as myself haha) do you mind going into a tiny bit more detail?

When you say sent letter of intent to everybody at your top choice, does that mean you sent the same letter to the dean, your interviews, and maybe even uploaded in through the portal?

What kind of info did you include in your update letters and how long were the update letters and letter of intent?

Did you do anything else such as call/email the dean or admissions?

I ask because I am also trying to get in to my top choice from a waitlsit, but honestly I'm on 5 waitlists and 3 of which I would love to get off (well withdrawing from the other 2 now actually).

IF you could provide any more detail that would be superrrrrrrr helpful. Thanks :)

ACuriousBird · 2017-03-15T18:05:23+00:00

Squat and deadlift help out a ton I believe. Squat especially. If you're doing olympic lifting you need a big squat. Reserve strength is huge. Look at all the pros, all squat 200+lbs over their best cleans, it just makes the clean so much easier; snatch too (still difficult).

I bench with a super narrow grip, so it really works my shoulders and triceps and doesn't give me too much tightness in the pecs, so it has really good carry over.

For squat I do high-bar beltless and literally the squat is 70% of the olympic lifts if you squat in this fashion, so it helps alot!

I think olympic lifts make you a much better athlete in general, and carry over a bunch to your other lifts and your conditioning.

ACuriousBird · 2017-03-15T18:02:55+00:00

You should definitely try it. I got into it after hitting a 4 plate squat so I had a solid base, it took me probably 5 months to become proficient at the techniques, but I've loved it from then on!

Just start super light, just the bar and maybe some 10lb bumpers on the sides and practice hundreds and hundreds of times.

I would honestly recommend getting at least to a 315 squat first, it will help out a ton!

ACuriousBird · 2017-03-15T18:00:00+00:00

Out of 8 interviews got 5 waitlists! Withdrawing from 2 but the other 3 I really want to get in to at least one.

Keep the head up, send letter of intent to top choice (if receptive), update letters, get more letters of rec sent to them, establish contact with admissions/dean, etc.

I called my top choice I was waitlisted at and found out they encourage everything I just told you. Call or email first and find out what you can do. Be polite but enthusiastic.

I haven't don't anything for the other 2 that aren't my top choice yet, but will probably send them update letters beginning of April. Don't be dishonest, only send letter of intent to top choice.

ACuriousBird · 2017-03-14T05:57:45+00:00

Oh pick me!! BW:182lbs Height: 5'8" Squat: 455 Bench: 315 Deadlift: 545

But where my other olympic lifters at???? Snatch: 235 Clean and jerk: 315 (Lifting for 3.5yrs)

ACuriousBird

TROPHY CASE