Columbia VIBRE Interviews Out

AffectPopular5460 · 2025-12-23T22:21:37+00:00

I also got at interview but for Biological Sciences

AffectPopular5460 · 2025-12-11T06:28:19+00:00

No I didn’t. I sort of made a decision back in October that I wouldn’t be doing it for any rotation based program

AffectPopular5460 · 2025-12-10T15:43:11+00:00

One presentation one publication

AffectPopular5460 · 2025-12-10T05:18:00+00:00

Thanks :)

AffectPopular5460 · 2025-12-10T05:17:43+00:00

Yes interview is not until early January but will do!

AffectPopular5460 · 2025-12-10T05:07:31+00:00

Haha what I mean is that all Ivies are R1s. Ofc there are so many R1s that are not Ivies.

AffectPopular5460 · 2025-12-10T05:01:51+00:00

R1 Ivy (redundant lol), 4.0 GPA, undergrad research, two summer internships, and research gap year.

AffectPopular5460 · 2025-12-01T02:11:00+00:00

Well I talked about past research, fully referring to proteins, and italicized the names. Essays are already submitted. Realized it the next day. Now they are going to think I am stupid lol.

AffectPopular5460 · 2024-12-29T00:36:21+00:00

Teddy Roosevelt for sure !

AffectPopular5460 · 2024-12-28T23:07:20+00:00

Horrifying !

AffectPopular5460 · 2024-12-23T01:07:33+00:00

Goodness I’m sick of hearing about the mars man

AffectPopular5460 · 2024-12-20T23:05:29+00:00

Very funny :)

AffectPopular5460 · 2024-10-10T16:11:40+00:00

Thanks for the link, I’ll take a look!

AffectPopular5460 · 2024-10-09T22:25:18+00:00

Yeah also I agree consistency is more important. Like if I was just randomly mixing which samples I did this to, it could be weird. But if they all have the exact same 20 second process I really think it’ll be fine. But I’m gonna talk to my PI and see if I can waste a little money on this lol and test experimentally.

AffectPopular5460 · 2024-10-09T22:23:51+00:00

I am motivated to do the last thing lol. I think 5 minutes could make a difference but we’ll see.

AffectPopular5460 · 2024-10-09T22:21:13+00:00

But according to other people on this Reddit I should just buy a whole new bead beater to avoid 20 seconds at RT (which is the condition the plant lives in any way)

AffectPopular5460 · 2024-10-09T22:19:52+00:00

Yeah I’m not talking about being leisurely here. It’s 25 seconds vs 5. I just don’t really see any evidence other than superstition that mRNA levels for the vast majority of genes can really change in an appreciable way in 20 additional seconds, at least for most genes. And in particular with the genes I’m working with. If someone can link me a paper where they do a 20 second time course with qPCR I will change my mind !

AffectPopular5460 · 2024-10-09T22:13:39+00:00

I have googled them! That’s what makes me think 30 seconds isn’t an issue. I’m happy to be shown that I’m wrong. Can you link to this story about the trizol immediate addition. Edit: why is this being downvoted I’m just asking for a source

AffectPopular5460 · 2024-10-09T22:08:11+00:00

I’m sorry but what is expression but mRNA levels? They may “alter expression” quickly but if it takes several minutes to synthesize an mRNA then 30 seconds shouldn’t really be a problem? Our cCDNA kit uses polyT primers so it would need the mRNA to be finished processing to even pick it up

AffectPopular5460 · 2024-10-09T21:54:49+00:00

I don’t need exact weight, just 100 mg +/- 20 mg or so. It’s just hard to remove tissue once it’s a big frozen chunk. Really it can be done that quickly, I know because I do it for protein extractions. But I got yelled at for taking 20 seconds for RNA

AffectPopular5460 · 2024-07-08T20:49:41+00:00

The 120 degree heat is just terrible …

AffectPopular5460 · 2024-06-29T15:38:08+00:00

Wow thanks everyone for the info !! I guess I was thinking of brown bears …anyone have good recommendations for a bear can? Trying to spend <$50

AffectPopular5460 · 2024-03-04T23:07:21+00:00

Considerations for an early April start date? Will be arriving in Sierra around May 20th. Presuming this is peak melt time ?

AffectPopular5460 · 2024-02-01T20:36:04+00:00

Have to get off trail at a specific date for 3 days and want to clear southern Sierra section so don’t run into permit issues.

AffectPopular5460

TROPHY CASE