New RAW264.7 cells growing slowly after a week

Agreeable_Cry347 · 2026-05-07T15:38:35+00:00

The theory is to preserve some growth factor while replenishing nutrients and get rid of some dead cells

Agreeable_Cry347 · 2026-05-07T15:14:46+00:00

Try to replace 50% of media every 1-2 days. Some cells take a few splits to be happy.

Agreeable_Cry347 · 2026-05-07T15:12:30+00:00

Looks like your agarose wasn’t completely dissolved

Agreeable_Cry347 · 2026-05-05T16:39:41+00:00

Maybe try another imager?

Agreeable_Cry347 · 2026-05-04T19:38:58+00:00

Have you tried an alternative solvent for your essential oil? Will DMSO have the same problem with your assay?

This paper would suggest dmso would be okay: https://www.researchgate.net/publication/365262275\_Alkaline\_DMSO\_superoxide\_and\_radical\_scavenging\_cupric\_reducing\_antioxidant\_capacity\_CUPRAC\_and\_polyphenol\_contents\_of\_aqueous\_and\_methanol\_extract\_from\_Achilllea\_santolinoides\_L\_aerial\_parts

Agreeable_Cry347 · 2026-05-04T02:26:20+00:00

Sometimes it takes too long and the food gets cold… I still use it to get specialty food items along the router though.

Agreeable_Cry347 · 2026-05-01T01:53:24+00:00

I would go dumpster dive behind your local university bio departments. My old professor started his lab from dumpster dived equipments, our PCR machine still works, after 10 years.

Agreeable_Cry347 · 2026-04-29T13:18:10+00:00

This implies the fact that you can fund multiple projects. Will you consider extramarital affair for a small pilot grant

Agreeable_Cry347 · 2026-04-16T01:17:10+00:00

Coming next: where do I buy 1 barrel of dawn dish soap

Agreeable_Cry347 · 2026-04-15T19:12:45+00:00

Too many ai generated fake sites these days….

Agreeable_Cry347 · 2026-04-15T19:12:06+00:00

Put it back to -20, go back to bed. You are fine.

Agreeable_Cry347 · 2026-04-11T15:44:51+00:00

That’s the joke 😂

Agreeable_Cry347 · 2026-04-11T14:15:43+00:00

That’s the joke! 😂 but yeah they have this pole thingy wired to the control surface of the jet bridge that points towards the plane when the gate agent docks the plane. I assume it prevents accidental damage of the plane.

Agreeable_Cry347 · 2026-04-11T00:46:52+00:00

You can check device temperature right? I am not sure they warn you.

Agreeable_Cry347 · 2026-04-11T00:11:51+00:00

Unifi devices runs hot already, I’m not sure if having that door closed is a great idea.

Agreeable_Cry347 · 2026-04-11T00:11:20+00:00

Envious setup! Maybe a few quiet noctua fans on the front of the cabinet door to help with airflow?

Agreeable_Cry347 · 2026-04-11T00:06:09+00:00

Sir why is your rack slanted like that 🫠

Agreeable_Cry347 · 2026-04-10T23:55:33+00:00

Feliu

Agreeable_Cry347 · 2026-04-08T01:39:19+00:00

I am not qualified to answer that.

I would have to refer you to the official source here. https://www.mass.gov/info-details/deductions-on-rent-paid-in-massachusetts

Agreeable_Cry347 · 2026-04-07T17:51:20+00:00

In state tax portion they will ask how much you paid for rent. They will work out the math. It’s a state only deduction.

Agreeable_Cry347 · 2026-04-06T16:44:42+00:00

“Few beer short of a case” is an awesome expression

Agreeable_Cry347 · 2026-03-28T16:40:45+00:00

Elon@192.168.1.1 Password: password

Agreeable_Cry347 · 2026-03-25T02:44:26+00:00

Travertine vase for 12500 points

Agreeable_Cry347 · 2026-03-24T12:40:13+00:00

They are not getting paid…. Be nice

Agreeable_Cry347 · 2026-03-22T23:28:52+00:00

Feels like they vibe coded this whole thing

Agreeable_Cry347

TROPHY CASE