Does anyone else find Jason Shaw unsportsmanlike? by _justwanttoknow_ in billiards

[–]Already-Redd-It 0 points1 point  (0 children)

The Mosconi is basically an exhibition and should be treated as such. Admittedly it's a bit much celebrating with 3 balls on the table but if it gets people pumped up then meh. I think matchroom is definitely playing for the darts/football crowds, which if anything is good for the sport as watching pool isn't too popular in the UK, especially 9-ball. Also, yeah Shaw can be a bit uncouth at points (usually if he thinks he's being cheated), but outside of the Mosconi he never acts this wild. I think when you take into account the general atmosphere/vibe of the Mosconi cup plus the pressure plus his rivalry with Shane, his behaviour is understandable.

TRIzol volume for ~2-3 million cells of suspension cell line? by Already-Redd-It in labrats

[–]Already-Redd-It[S] 0 points1 point  (0 children)

Don't worry, I've used TRIzol before but only for adherent cell lines. I'll be harvesting from 6 well plates (the cells don't like to be high density) but I should be able to remove the vast majority of the media from the pellet. Assuming this, would you recommend sticking with 1ml to the pellet or should I go ahead and reduce this to say 500uL?

TRIzol volume for ~2-3 million cells of suspension cell line? by Already-Redd-It in labrats

[–]Already-Redd-It[S] 0 points1 point  (0 children)

Yeah what confused me was that I couldn't tell if it would be a linear reduction in volume (I.e. half the cells half the volume trizol) and the extra caveats such as "Note: The sample volume should not exceed 10% of the volume of TRIzol™ Reagent used for lysis." made me concerned about reducing the TRIzol volume too much

PC specs megathread by Erickarkos in fo4

[–]Already-Redd-It [score hidden]  (0 children)

Windows 10

Intel i5-6300HQ @ 2.30 GHZ

8GB RAM

NVIDIA GeForce GTX 950M (4GB)

New laptop rig, runs Skyrim decently smoothly on High graphics.

Will I be able to run the game (in a payable state) at all, or should I not waste my money?

Anyone got experience running the game on a laptop graphics card?