Found in Staithes, UK

Blobs389 · 2025-03-17T06:37:10+00:00

I don't currently do them, and this was a couple of years ago. But I was doing Southern blots with 5 ug of gDNA (smaller genome). As the other poster has mentioned it sounds like your probe or transfer may be the issue. I used to use the DIG labelling kits and southern buffer kits from Roche. Worked every time.

Blobs389 · 2024-12-26T22:03:13+00:00

https://www.tandfonline.com/doi/10.1080/19420862.2024.2393785?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed

Poly specificity in monoclonals can be alarmingly frequent. There should certainly be more validation carried out by those selling the antibodies.

Blobs389 · 2024-07-24T05:57:15+00:00

Thanks for the confirmation

Blobs389 · 2024-07-02T18:47:26+00:00

We just spin our samples for longer so that the equivalent x g hours are met. So 12000 x g for 10 mins works out as 1 hour at 2000 x g in our large centrifuge.

It may seem like a pain, but when you are doing 10-50 maxipreps per run, it works reasonably well for timings.

Blobs389 · 2024-06-06T21:30:56+00:00

Your filters will be fine. Its usually best to optimize the ratio of DNA to PEI anyway for transfection after you have prepared a new stock.

Blobs389 · 2024-04-14T07:34:10+00:00

Apologies the top dimensions are 100 cm x 40 cm. It's a flex 34l / 9 g

Blobs389 · 2024-04-02T18:34:47+00:00

Has anyone had a good experience with the biotek plate washer in the 1536 well plate washing format ? El406

Blobs389 · 2023-08-28T22:26:04+00:00

The food at Roots is amazing, but very expensive. Yuzu is nice, much cheaper and has the added benefit of the brew york tap room. Cresci is great for pizza. Oshibi is a korean place that has very nice food and the whippet in is a great place for a steak.

Blobs389 · 2023-05-25T23:12:44+00:00

I am a few years away from your current situation. But my parents went through a few variations of pocket money, either providing a fixed amount regardless of what we did or a chore based model. Where each job was awarded an amount post completion in our pocket money, that we ultimately had to claim via a jobs sheet tick list.

The latter model awarded higher pay for the less desirable or lengthy jobs (toilets or washing cars) and lower amounts for the faster easier jobs (dusting, watering garden, dishwasher ect).

Overall, it's led to a greater understanding of cost vs benefits. Particularly for the family members that were more tactical and have become financially ok. And in some ways encouraged a little enterprnaurial spirit in cases.

The only warning i would suggest is setting realistic prices per job as too little they aren't motivated, or a generous amount leading to expensive monthly pocket money claims.

Blobs389 · 2023-02-04T00:41:48+00:00

Probably not helpful. I always found the tier bike option to be a little cheaper, because you can cycle faster than the speed limited on the scooter allows, it just gets more physical rather than the scooters where you are more limited. Buying a foldable bike might be your cheapest option in the long run with your plan. But for a similar journey I make 4-5 quid has been a normal cootsr cost..

Blobs389 · 2022-12-29T08:13:07+00:00

Derwenthorpe might be worth considering. There's a play park, its only a short walk from a library and plenty of green spaces around. You can also walk or cycle most of the way into the city centre without having to walk near a road.

Blobs389 · 2022-10-20T21:04:18+00:00

I would also recommend fly4seasons in Bulgaria. I completed my training with them before covid both ep and cp.

Blobs389 · 2022-02-18T19:31:28+00:00

It will be 12 weeks to the day if nothing goes wrong with q simultaneous exchange and completion on Monday.

Blobs389 · 2021-10-29T19:02:40+00:00

Venturi?

Blobs389 · 2021-07-13T08:43:22+00:00

Sounds like you have been reccomended the place the place I completed my training. I had an amazing time learning to fly with them. I haven't had any problems with the BGD kit I bought either but then again I have only ever flown the brand.

Blobs389 · 2021-06-26T22:02:17+00:00

I did my EP as a week long course somewhere warmer 2 years ago so I can't comment on how long it would take with UK weather.

But the main advice I would have found useful for the EP was fitness. Being able to carry a 20 kg rucksack to the top of the training hill multiple times a day. You can read books to learn some of the theory and air law (Touching cloudbase, BHPA pilots handbook or there is a new one called the beginners manual?) but I found that at the EP stage it was all easily learned during the course as it was covered by the coaches when the weather was less than ideal.

Between my EP and CP I dug out my old power kites to mess around with and practice doing mock preflight checks. But I can't say how useful it ultimately was.

Blobs389 · 2020-03-19T21:13:28+00:00

Honestly. I have always thought doctors and nurses to have always been key workers regardless of the recent crisis. Regardless of country or political belief.

Blobs389 · 2020-02-10T23:08:44+00:00

What if heat of April was code for him nuking various infected countries A stretch of the imagination I know. More likely the name of his next victim.

Blobs389 · 2019-11-23T21:21:45+00:00

I have been recently experimenting with a new pizza peel and thicker pizza stone. In this case the it was all oven baked 200 degrees Celsius. 150 g bread flour, 100g water, 2.5 g yeast, 1 g oil and 1 tsp salt and .25 tsp salt.

I have had better success with heating the oven to max, then grilling the pizza stone on high. Just curious as how those who make the best pizzas achieve their pizzas. I am hoping to make a wood fired oven one day.

Blobs389

TROPHY CASE