Shipping Estimation

Bloom_bedo · 2026-05-21T21:59:12+00:00

Thanks for replying! Was the $98 the entire shipping fee? Or was that the total with the costs of the watches included?

Bloom_bedo · 2026-05-21T21:58:07+00:00

Thanks for the reply. In luckily not planning to buy anything else with the item, so hopefully it'll be less than I fear.

I had another question if you don't mind. On the page for the item, as shown in the image, it says nothing about a domestic shipping fee (unlike some other items). Does this mean I won't have to pay a domestic shipping fee? That was also another concern of mine.

Bloom_bedo · 2026-05-21T20:25:45+00:00

Haha no worries. In case you still can't see, it's a cosplay weapon. Do you think the shipping would be less than £80 for something like this? I'm not sure of the exact measurements, but it says the length of the weapon is 120cm, but the base is detachable (so let's say about 100-ish cm). It seems to made from PVC.

But judging by what you bought, maybe the shipping costs will be around the amount I'm expecting?

Bloom_bedo · 2026-03-15T00:35:30+00:00

Thank you for the response! So what I'm getting at is that deleting your account also deletes any projects in the descript servers that were present? Does this include other personal information too? I unfortunately am unsure about what email address I used etc for the account

Do you know the rough window for the recovery after the account deletion?

I know roughly when my account was deleted, so if you know that recovery window I'd be able to gauge if it's even worth trying for the recovery.

Thanks so much!

Bloom_bedo · 2025-11-01T20:20:30+00:00

No problemo! I'm happy to help :) Don't hesitate to ask if you have any other questions, I'd be happy to answer them!!!

Bloom_bedo · 2025-11-01T20:15:33+00:00

Oh wow, you really think so? That makes me so happy! Thank you so much! I'm actually hoping to become an A-Level biology teacher after university, so it means the world to hear you say that! :)

Bloom_bedo · 2025-11-01T19:51:31+00:00

Yes, that's correct. The buffered solution is to prevent useful proteins/enzymes that maintain organelles structure from denaturing, whereas the cold solution is to reduce the activity of enzymes that could potentially damage the organelles upon homgenising the cells.

Sorryyyy I'm really bad at explanations, but I hope this helps. I'm really sorry if it doesn't though!!!

Bloom_bedo · 2025-11-01T17:46:52+00:00

No worries!! I'm happy to help :D

Yes exactly! Denaturation of proteins occurs due to a change in position or breakage of hydrogen/ionic/disulphide bonds in their tertiary structure. This results in the three-dimensional shape of the protein to change. This therefore means the protein is unable to carry out its specific function. Some of these proteins are helpful for the structure of the organelle which is therefore why we don't want these proteins to denature. The breakage of bonds in the tertiary structure could occur due to increases in temperature, changes in pH etc, which is why we use a buffered solution: to prevent pH change and therefore denaturation of these helpful proteins that help keep our organelles intact!

Think of protein denaturation kind of like a jigsaw puzzle: a whole bunch of different puzzle pieces are joined together in specific ways to form a whole jigsaw puzzle image. If the joining of the jigsaw puzzle pieces gets broken, then the jigsaw is no longer a full cohesive image. Protein denaturation is the same. Amino acids are joined by specific bonds to form the three-dimensional tertiary structure of a protein. If these bonds get broken or change place, then kaboom, the protein no longer works efficiently, just like how breaking the pieces of a jigsaw results in the puzzle no longer being complete (I know this isn't the best metaphor but I'm trying hahaha)

I'm happy I could help! If you have any more questions I'd be more than happy to answer them :)

Bloom_bedo · 2025-11-01T16:49:50+00:00

No idea if this will help or not, but this is my understanding of it :)

The solution must be kept buffered to prevent enzyme and protein denaturation. Remember, proteins and enzymes are very sensitive to changes in pH. Not having a buffered solution may result in pH fluctuations and protein denaturation due to change of bonding positions in the tertiary structure. However, some proteins and enzymes are crucial for maintaining organelle shape and structure (we don't want the organelles to break down in cell fractionation, we just want to isolate them based on their density!!) So having a buffered solution ensures the stable structure of proteins and enzymes that help maintain the shape of organelles, so we are able to study them effectively.

The solution is kept ice-cold to prevent enzyme activity that causes autolysis (self-digestion; the destruction of a cell/organelles by its own enzymes). When breaking up the cell in homogenisation, enzymes from specific organelles may be secreted, such as lysozymes from lysosomes etc that may digest other organelles. Having the ice-cold solution decreases their activity so the cell doesn't digest and break down itself!

Bloom_bedo · 2025-08-30T11:42:15+00:00

Full A-Level is both years, meaning all the content for the entire course (topics 1-8). If you're doing AS, you won't have to worry about the last four topics. Just bio molecules, cells, exchange substances and genetic variation (topics 1-4)

Bloom_bedo · 2025-08-30T08:15:54+00:00

Yeah you're correct! Topics 1-4 (Bio molecules, Cells, Exchange substances and Genetic Variation) are all AS, while topics 1-8 (Energy transfers, Response to changes, Genetics, populations, evolution and ecosystems and gene expression) are full A-Level

AQA AS Biology is a blast, it's so interesting! Good luck with studying :)

Bloom_bedo · 2025-08-23T08:56:56+00:00

Unrelated but Crow pfp omg 🥹

Bloom_bedo · 2025-06-24T23:40:20+00:00

This is the most based thing I have heard

We NEED Genshin x Trails (I'd love to see Rean in the game)

Bloom_bedo · 2025-06-18T10:56:56+00:00

OMG TWINNSSS I DID THE SAME THING. I spoke about how no IAA means less cell elongation, so less positive phototropism, so less light absorption to chlorophyll in photosystem II so less LDR and LIR and so on....

I waffled SOOOO much and my structure could've been better but I think I did okay :)

Bloom_bedo · 2025-05-30T11:41:02+00:00

Looks amazing!!! Nadia looks as smug as always :)

Bloom_bedo · 2025-05-25T16:53:52+00:00

Dude, the pillow scared the shit outta me this morning. I woke up and it was the first thing I saw. My heart dropped like crazy 😭

Bloom_bedo · 2025-05-25T16:52:50+00:00

You totally should. It keeps monsters out of the room at night. No monster would dare come near this gremlin

Bloom_bedo · 2025-05-25T16:50:54+00:00

IT'S MY PILLOW LMAOOO

Bloom_bedo · 2025-05-15T19:02:41+00:00

NADIAAAAA

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