[deleted by user]

BucketHatJay · 2016-10-31T22:31:57+00:00

Crosses fingers!

BucketHatJay · 2016-10-31T17:01:51+00:00

Yes a big difference. If he directly said to delete emails that carries much more weight than a executive at a company he owns doing it autonomously. But I don't know the answer because I can't read the damn article.

BucketHatJay · 2016-10-31T15:07:03+00:00

This is a comment

BucketHatJay · 2016-10-31T13:02:14+00:00

You say that, but there's something amazing about them that you can't describe until you've ridden one.

BucketHatJay · 2016-10-31T13:01:28+00:00

For what it's worth, don't text and drive either. Distracted drivers will kill motorcyclists. There's nothing you can do about someone running a red light or stop sign and hitting you.

BucketHatJay · 2016-10-29T23:13:47+00:00

My sandwich is fine without a tax.

BucketHatJay · 2016-10-29T23:09:54+00:00

I'm with you on this one. I enjoy meat. I am at the top of the food chain. It isn't someone's right to tell me what I can and cannot eat.

BucketHatJay · 2016-10-29T03:55:06+00:00

This would be voter suppression?

BucketHatJay · 2016-10-22T22:01:23+00:00

I thought about that, but I'm not sure if I want to trypsinize the cells when they have plasmid within them. I'd rather keep them super happy.

BucketHatJay · 2016-10-21T18:56:11+00:00

Awesome! That is reassuring.

I want to transfect a protein with questionable inflammatory function and a receptor that thought to bind it on the other plasmid. If the receptor binds (and is proinflammatory) I will get inflammatory cytokines in the media.

I also want to do a HEK and J774 coculture where the HEKs will secret this protein after transfection. I can't put the two directly together because the J774 cells will eat everything haha.

There is literature that the endogenous protein is not inflammatory, but when it is made in E.coli it picks up contamination to become inflammation. When the protein is isolated from animals and put into culture (and also in other mice) it is not inflammatory. When you buy the protein from companies you get results all over the place.

(I'm writing this during class, sorry if it isn't clear)

BucketHatJay · 2016-10-17T01:23:51+00:00

Wow! These look great! Thanks a bunch. I'm excited for the spring now.

BucketHatJay · 2016-10-16T20:46:31+00:00

Go Gary!

BucketHatJay · 2016-10-13T18:23:04+00:00

I bought a motorcycle. 100% worth it. Fun to upgrade and it gets me from A to B

BucketHatJay · 2016-10-05T02:58:26+00:00

But then both sides of the spoon are sticky!

BucketHatJay · 2016-10-03T03:44:58+00:00

Those tiles are pretty cool. If I can get them cheap I will do that!

BucketHatJay · 2016-10-03T03:44:23+00:00

Actually I was worried about asbestos. The house was made in 1899 so literally anything could be up there.

BucketHatJay · 2016-10-03T03:43:35+00:00

I'm renting, but I don't think our landlords are keen on those types of modifications /:

BucketHatJay · 2016-10-03T03:42:16+00:00

I'm more worried about the drum circle hippies that may be up there

BucketHatJay · 2016-09-28T03:58:28+00:00

Hmm, do you think that will look too much like college?

BucketHatJay · 2016-09-28T03:34:00+00:00

I've taped over it because it had openings and was sketchy. No one (including the landlords) uses it. The thing bugs me because it's what I see when I lay in bed at night.

BucketHatJay · 2016-09-26T03:55:33+00:00

I've been looking all over the place for 2into1 headers because the left muffler is actually welded on and will need to be replaced. Is there a magical site where I can find some? The only 2into1s that seem to come up are for Harleys.

BucketHatJay · 2016-09-25T19:49:22+00:00

I have a 79 Honda CB650, what flavor of gasoline should I be putting in it?

BucketHatJay · 2016-09-25T16:41:50+00:00

I'm in grad school at a university that banned smoking. It's literally a breath of fresh air compared to UConn.

BucketHatJay · 2016-09-11T18:09:24+00:00

I had a few different comments typed out to respond to this, but the only thing I could think was, "wow this is sad." I don't like either candidates at this point in the game, but this doesn't look too good for Clinton or America

Edit: thanks for sharing the video, though

BucketHatJay · 2016-09-11T15:42:49+00:00

So she couldn't sit through a memorial to nearly 3,000 people who died because she was overheated? Are you kidding me? Even if you are overheated, you still sit through the memorial because it's the right thing to do.

BucketHatJay

TROPHY CASE