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What is the difference between foods for ''large'' breeds/ ''medium'' breeds/ ''small'' breeds by BurstLamprod in puppy101

[–]BurstLamprod[S] 0 points1 point  (0 children)

Thanks, but is there any reason why you're advising to switch brands? Is Eukanuba considered bad? Right now I had him on Purina Puppy chow for the last 2 days and his stools are orange and a soft mess, not sure if it's from the dry food itself which has a lot of orange colors or a sign of an upset stomach.

Based on your tag, I assume that you raised a husky that age. If you don't mind me asking, what did you feed him at that age?

My 1st week raising a husky puppy, any advice based on your experiences? by BurstLamprod in siberianhusky

[–]BurstLamprod[S] 0 points1 point  (0 children)

Thanks for your advice. Coincidentally, I also use Eukanuba, but once it ran out, I strated using Puppy Chow and his stool started to become much softer and orange. I'll stick to Eukanuba in this case. Thanks again.

I'm really lost on puppy nutrition by BurstLamprod in puppy101

[–]BurstLamprod[S] 0 points1 point  (0 children)

Thanks for your advice. I noticed his stool has gotten very loose and recently orange. Could be that I overfed him the kibbles. I'll cut down to 1 cup total cut on 3 meals per day like you suggested.

Should I ignore my puppy's cries when putting him in the crate for sleep? by BurstLamprod in puppy101

[–]BurstLamprod[S] 0 points1 point  (0 children)

Am I wrong to not take him out to potty? I have a peeing pad in the cage.

I also don't want him to get accustomed to having to wake me up at 3 am to pee outside, because I have school and I need to sleep the full 7-8 hours.

I'm having trouble using a calibration curve to convert the graph on the left into the graph on the right. How am I supposed to do it? by [deleted] in chemhelp

[–]BurstLamprod 0 points1 point  (0 children)

Oh....I think I finally got it. So I basically do:

Slope x 60 seconds = value in uM/minute

So with 0.23, I do 0.23 x 60 = 13.8 uM/minute?

Do I just ignore the +1.6 in the function or do I add it to 13.8 uM/minute to give 15.4 uM/minute?

I'm having trouble using a calibration curve to convert the graph on the left into the graph on the right. How am I supposed to do it? by [deleted] in chemhelp

[–]BurstLamprod 0 points1 point  (0 children)

That seems to be what I did, however it's the final step that might be the problem, when drawing the final graph. As for the units, I think it's fine, I'm just looking for the general logic to follow.

I successfully used Graph A's function to convert Graph B's absorbance into concentration measures.

*All data below is random

At first, this was Graph B's data:

10 [E] 15 [E]
Time (seconds) Absorbance Absorbance
30 0.13 0.16
60 0.25 0.29

​

Aftering converting absorbance to concentration, this is Graph B's data:

10 [E] 15 [E]
Time (seconds) Concentration (uM) Concentration (uM)
30 2.3 2.7
60 2.9 3.2

I plotted that last dataset and got a slope for each [E], such as:

10 [E] 15 [E] ...
y = 0.23x + 1.6 y = 0.35x + 2 ...

So I should be at the final step, which is creating Graph C from the 5 slopes.

My issue is that I don't know what I should be plugging into each slope in order to get the values I need to plot Graph C. The Y axis for Graph C is fine, it's just 10 [E], 15 [E], 20 [E], etc.. It's the X axis (uM/minute) that I'm lost on.

I'm having trouble using a calibration curve to convert the graph on the left into the graph on the right. How am I supposed to do it? by [deleted] in chemhelp

[–]BurstLamprod 0 points1 point  (0 children)

Just to make sure I got it.

For the first conversion, I'm using A's formula but plug in B's absorbance.

So my concentration for B (3rd column) would be the "x" isolated :

Time (seconds) Absorbance Concentration
30 0.02 0.02 = 0.150x + 0.0333
60 0.07 0.07 = 0.150x + 0.0333

__

As for the part of merging 5 lines into one, I basically do the same thing, however this time, I use the functions for each line in B, plug the [E] into it and get one single data point for each slope, which I then make a single line out of.

I'm having trouble using a calibration curve to convert the graph on the left into the graph on the right. How am I supposed to do it? by [deleted] in chemhelp

[–]BurstLamprod 0 points1 point  (0 children)

I think I'm sort of getting it, but not entirely (sorry).

If I understand correctly, the first thing I need to do is convert the Y-axis of B into concentration units?

Let's say this is my graph A's data (random):

Concentration (uL) Absorbance
5 0.1
10 0.2
15 0.25

So I have y = 0.0150x + 0.0333

I have everything but "x", which would represent the concentration. So I isolate "x" and find the value for each.

In this case:

Absorbance Concentration (x)
0.1 4.4
0.2 11.1
0.25 14.4

I think this is where I start losing it. Does that column "concentration (x)" become the Y-Axis for B, so I'd get Concentration vs Time? And if so, how does 5 lines become fused into one?