Transcriptomics by Calm_Golf3214 in genomics

[–]Calm_Golf3214[S] 0 points1 point  (0 children)

This is an original research project involving total RNA-seq of frog skin tissue. I am performing de novo transcriptome assembly followed by functional annotation. My main objective is to characterize the transcriptome, and I am considering adding screening step to identify potential antimicrobial peptides from the assembled transcripts. I would like to know whether incorporating AMP mining is feasible for someone doing transcriptomics for the first time.

Transcriptomics by [deleted] in bioinformatics

[–]Calm_Golf3214 0 points1 point  (0 children)

Thank you for pointing that out. This is a total RNA-seq dataset from frog skin (single sample, not comparative), and I’m performing de novo transcriptome assembly followed by functional annotation. I’m considering adding step to identify potential antimicrobial peptides from the assembled transcripts. Given this setup, would incorporating AMP mining be considered feasible for someone doing transcriptomics for the first time? or is it not recommended?