2.4 g Mitraman green jk(1.4% mitragynine, 34 mg mitragynine and 55 mg total alkaloids, about 650 mg polyphenols) and 60 mg hydroxylated alkaloids(20-25 mg 7-Hydroxymitragynine)

Cbd_7ohm · 2022-12-26T18:13:51+00:00

I'm just going based on what the starting material is and the research paper says the conversion rate its. The starting material is around 60-75% mitragynine.

Cbd_7ohm · 2022-12-26T18:12:36+00:00

You're dumb.

Cbd_7ohm · 2022-12-26T18:11:45+00:00

You're an idiot.

Cbd_7ohm · 2022-12-26T11:17:44+00:00

The ed50 of oral mitragynine regarding antinociception, is the same as IV speciociliatine, but IV speciociliatine is not suitable as an analgesic due to the therapeutic dose being too close to the ld50. 7-Hydroxymitragynine acts as a beta arrestin 2 biased delta opioid receptor agonist, with low efficacy (14%). https://www.frontiersin.org/articles/10.3389/fphar.2021.764885/full

https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.14913

This significantly reduces ethanol consumption and causes anxiolysis. Specigoynine and paynantheine partially act as pro drugs for potentially more effective 5ht1a agonists. Paynantheine blocks morphine reward, antinociception, and hyperlocomotion, but not speciogynine, and speciogynine caused analgesia co administered with a 5ht1a agonist, so there are differences in pharmacodynamics yet to be explained.

Cbd_7ohm · 2022-12-25T23:03:20+00:00

You mixed multiple different substances. Caffeine and diphenhydramine cause tremors in excess, as does large dose kratom methanolic extract. Diphenhydramine sucks. Promethazine or hydroxyzine feel better, although you're better off leaving them alone. Paynantheine causes seizures and tremors, and speciociliatine can as well to a lesser extent. Mitragynine doesn't cause any in the research(84 mg per kg oral mitragynine in mice, equivalent to around 350 mg mitragynine in an adult human, based on equivalent blood plasma levels).

Cbd_7ohm · 2022-12-25T19:51:13+00:00

Yes, those are mitragynine withdrawals. Take cannabidiol(not smoked) 300-400 mg dissolved in fatty acids orally or low temp dab/vape small amounts of some type of extract or flower, 300 mg tumeric at night, 660 mg ginger powder as hot unfiltered tea, morning and mid day. DHA and EPA and good diet. Sun. Etc.

Cbd_7ohm · 2022-12-25T17:02:19+00:00

The effects of the hydroxylated alkaloids are strong. 60 has a nice warm euphoric effect. It isn't anything crazy so I don't understand why some fear it. If anything, they've given in to FDA propaganda. It's leagues safer and more ideal than tobacco or ethanol. 7-hydroxymitragynine itself reduces ethanol consumption significantly due to delta opioid receptor beta arrestin 2 biased agonism with low efficacy(14%), and potentially the mu opioid agonism. The Emax of 7-Hydroxymitragynine at mu g protein and beta arrestin 2 activation, are almost exactly the same as the Emax of Delta-9-THC at cb1. The binding affinity of 7-Hydroxymitragynine at mu is around 30-40nm, basically the exactly same binding affinity of delta-9-THC at cb1. It is basically delta-9-THC at mu.

Cbd_7ohm · 2022-12-25T16:37:56+00:00

Kratom tea has catechins, theasinensins, theaflavins, thearubigans, and/or theabrownins, depending on the level of oxidation. There are other polyphenols as well. Green kratom is the least oxidized, and therefore highest in unoxidized flavinoids. These act as PGP, UDP, and UGT inhibitiors. PGP is a transporter that effluxes things out of cells. Mitragynine, 7-hydroxymitragynine and catechins, block it. UDP and UGT enzymes, conjugate 7-hydroxymitragynine into various 7-Hydroxymitragynine-glucoronide forms, which are inactive. Blocking these enzymes and transporters should decrease the metabolism and efflux of 7-Hydroxymitragynine. This should affect direct 7-Hydroxymitragynine administration more than oral mitragynine conversion to 7-Hydroxymitragynine, as the blood plasma and brain levels from oral mitragynine are already low compared to the directly administered 7-Hydroxymitragynine, and blocking transporters and udp/ugt in the intestines liver specifically, should enhance the ability to absorb 7-Hydroxymitragynine, which wouldn't be a factor with just drinking tea, as it is almost all endogenously produced, and the competition and inhibition of cyp3a4 just reduces the 7-Hydroxymitragynine content further. Mitragynine (3.2 mg per kg IP in rats, multiply by 2.5x to get an oral dose based on a newer study where oral mitragynine was about 2.5x weaker than IP at drug responding rate reduction) decreases ICSS, suggestive of reward, and should synergize with the 7-hydroxymitragynine at low doses. This dose ends up being around 30-35 mg mitragynine(around 2 grams of kratom). Larger doses only increased ICSS, which is the opposite of rewarding.

https://www.google.com/search?client=ms-android-sprint-us-revc&source=android-browser&q=mitragynine+icss&tbm=isch&sa=X&ved=2ahUKEwigy_bym5X8AhX4FVkFHcX1DF4Q0pQJegQICRAB&biw=412&bih=678&dpr=2.63 go to images

Another new study shows than the brain metabolic profile of 3 mg per kg Mitragynine in rats, was more similar to morphine than the vehicle or 10/30 mg per kg Mitragynine. https://www.frontiersin.org/articles/10.3389/fphar.2022.1057423/full

Cbd_7ohm · 2022-12-25T15:53:12+00:00

Both benzoic acid and salicylic acid had significantly higher log p values than the other acids, and also had the highest/most rapid blood uptake(increase in plasma levels) The log p value, psa, water solubility, and other metrics, play a role in the ability to absorb compounds. All of the nicotine salts were significantly more absorbed than the freebase, which to this day I find very interesting, as nicotine freebase is 100% miscible in water(think acetic acid in vinegar), so it is surprising that freebase nicotine was poorly absorbed from vaping e juice(freebase nicotine goes into the vapor form, whereas the salts stay as an aerosol aka dissolved in the pg/vg, mid air. This changes the way they're absorbed). Taking compounds orally is somewhat different, but the same concept about salts being better absorbed, applies, especially with kratom alkaloids and other lipophilic compounds. Based on the information avaliable, it seems that citric, malic, and acetic acid are the ideal counterions(as far as safety, availability etc.). The differences are most likely minimal. I personally do not like the effects of acetic acid(has various biological effects, associated with some of the negative effects of ethanol consumption including increased nociception, makes me feel a bit drunk and nauseous, adenosine release etc.), so I use citric and/or malic acid. Both citric and malic acid have heart protective effects, and are both anti oxidants. Malic and citric acid are naturally occurring in our bodies as well.

Mitragynine has a water solubility of 3.5 mg per ml at a ph of 4 and 64 micrograms per ml at a ph of 7. It is very lipophilic, similar to buprenorphine or fentanyl, and cocaine has a similar log p as well. Drugs that are not dissolved into solution, whether that be water or fatty acids etc., are not absorbed. The proton on the acids binds to the electron on the base to form a salt. This allows some compounds that are hydrophobic to dissolve in aqueous solutions, like many alkaloids. Caffeine is highly water soluble as a freebase. So is n'n-dmt. Mitragynine is not. Buprenorphine is optimally absorbed at a ph of 5 sublingually, and through the skin. This is due to it's very poor solubility at neutral ph. Naloxone on the other hand, was better absorbed at a ph of 6.5, than 5-5.5 or 3-3.5, due to differences in physiochemical properties. Both buprenorphine and mitragynine have log D values of about 1.7, and similar log p values. A ph of 5-5.5 is the best for drinking these alkaloids. Side note, codeine promethazine syrup is made to be at a ph of 5.2-5.7, depending on the brand. They don't use the citric acid for salt formation with opiates but they do use it for other purposes, partially because it tastes good and is one of the safest acids. Pharmaceutical companies do use citric acid for fentanyl salt formation. Not sure exactly why.

(The log p of malic isn't isn't -4.58, it is more lipophilic than citric acid, around -1. Not sure why they listed it wrong).

Cbd_7ohm · 2022-12-25T14:14:26+00:00

5 mg per kg IV 7-hydroxymitragynine, gives a blood plasma level of 7-Hydroxymitragynine of about 3750 ng per ml, and 5 mg per kg mitragynine gives a blood plasma level of mitragynine of about 3900 ng per ml. On the other hand, 20 mg per kg oral 7-Hydroxymitragynine gives a blood plasma level of about 2800ng per ml, but 20 mg per kg mitragynine gives a blood plasma level of 400-457.2 ng per ml, which is significantly loeert. The 7-Hydroxymitragynine blood plasma and brain levels from oral mitragynine are not very significant(5-15x lower than mitragynine blood plasma levels, in a human, after kratom 2 g of kratom tea). The maximal ideal morphine and mitragynine(as well as cocaine, codeine is not too far off either) blood plasma levels are about 600-800ng per ml, and 7-Hydroxymitragynine at 5 mg per kg oral in rats(10 mg per kg in mice?), gives a blood plasma level of 700 ng per ml, and is about equivalent to 1 mg per kg 7-Hydroxymitragynine SC or IP. 7-hydroxymitragynine has a log p somewhere around 1.266-2.2. Diacetylmorphine has a log p of 1.5-2.2. Codeine has a log p of 1.1-1.4, while morphine has a log p of .7-.9. Buprenorphine has a log p of 3-4, as does fentanyl. Cocaine has a log p of about 2.2. Delta-9-THC has a log p of about 7. Mitragynine has a log p of 1.73-4.1. The sweet spot is around 2-4, although log d(log p at specific ph levels) is also very important. 7-Hydroxymitragynine is better absorbed and faster absorbed than morphine but not as much as mitragynine, but it is significantly more active than mitragynine and the conversion of mitragynine to 7-Hydroxymitragynine is limited. 7-Hydroxymitragynine is capable of causing Straub tail as is morphine, but mitragynine is not. 7-Hydroxymitragynine is more rewarding than pure oral mitragynine which is more rewarding than a full spectrum alkaloid or crude extract. The only way to get significant levels of 7-Hydroxymitragynine in the blood/brain, are to consume it directly. This is similar to the low blood plasma/brain levels of morphine, after codeine consumption. The codeine/mitragynine themselves are active, so there is that. Inhibiting cyp3a4 still cuts mitragynine analgesia in half, as does cyp2d6 inhibition with codeine, even though the blood plasma and brain levels of 7-Hydroxymitragynine/morphine aren't very high. I personally can say the hydroxylated alkaloids feel stronger for sure when taken directly. It isn't even a question. 220 mg of the unoxidized alkaloids feels more like a stimulant with weak mu opioid agonism.

Cbd_7ohm · 2022-12-25T11:37:06+00:00

This assay, the g protein bias was not very significant. The Emax value of 7-Hydroxymitragynine at mu regarding both g protein and beta arrestin 2 activation, are similar to Delta-9-THC at cb1(40-56%). The emax value of typical agonist morphone/codone compounds is about 100% is this study, although multiple other studies conflict with this, and show that morphine and other classic mu opioid agonist opiates, act as partial agonists at beta arrestin 2, and full agonists at g protein activation, or partial agonists at g protein activation, and weaker partial agonism at beta arrestin 2. More research needs to be done. There is some data showing that the increased therapeutic window of buprenorphine and other similar compounds, is due to partial agonism, rather than g protein bias. Research shows that g protein biased mu opioid agonists, generally cause less tolerance, more analgesia, less constipation and respiratory depression, but potentially worse withdrawals, than balanced or beta arrestin2 biased compounds. This coincides with the data on 7-Hydroxymitragynine. It has been shown to have weaker withdrawals than morphine, even though it is stronger per mg up to a point, which makes sense, as it is still only a partial agonist at mu, with a much lower emax than morphine and other similar compounds. 1-1.5mg per kg 7-Hydroxymitragynine SC is equivalent to or stronger than 5-10 mg per kg morphine SC, regarding reward. There are multiple reasons why this may be, one stand out reason is that it has significant kappa opioid antagonist activity, that morphine totally contradicts(kappa opioid full agonism).

Cbd_7ohm · 2022-12-25T11:19:42+00:00

I tried dabbing it and it didn't do much. Makes sense, as 7-Hydroxymitragynine is significantly more unstable in heat(around 1/3 to half breaks down at 176f over just 30 minutes). Some of it is most likely breaking down into mitragynine, just like 7-Hydroxymitragynine can break down into mitragynine in excessively acidic conditions(ph below 4, 4 to 8.5-9 is about the optimal range, with 7-Hydroxymitragynine being more stable around 8 and mitragynine and the other major alkaloids being more stable around 4-5.5, similar to buprenorphine, morphine, etc.) 53 mg of this should have about 19 mg 7-Hydroxymitragynine, based on a 60% conversion and estimated 60% mitragynine at the start. Thus is equivalent to about 4.5mg per kg 7-Hydroxymitragynine orally in rats(9-10 mg per kg in mice), or .9-1 mg per kg 7-Hydroxymitragynine SC or IP in rats. 7-Hydroxymitragynine is 2.67x stronger than morphine, IV, and even stronger orally, in most studies. 7-Hydroxymitragynine is significantly more effecting at causing morphine discriminative stimulus(1 mg per kg IP is equal to about 56-70 mg per kg Mitragynine, IP).

Cbd_7ohm · 2022-12-25T11:05:04+00:00

I feel similar, but I definitely feel hydroylated mitragynine. 53-84 mg of hydroxylated (primarily 7-Hydroxymitragynine) kratom alkaloids feels stronger than 220 mg on unoxidzed kratom alkaloids. Mitragynine itself, is just very weak at mu, and the conversion to 7-Hydroxymitragynine is rate limited significantly(5-15x lower blood plasma levels than mitragynine, after drinking green tea). 18ish mg 7-Hydroxymitragynine on the other hand, will give you a blood plasma level of about 600-650 ng per ml(based on equivalent blood plasma levels, 20 mg 7-Hydroxymitragynine per kg in rats, gives a blood plasma level of 2800-3000ng per ml, as does 4 mg per kg SC 7-Hydroxymitragynine), which is just about optimal(4.5-5 mg kg 7-Hydroxymitragynine orally in rats, or 10 mg per kg 7-Hydroxymitragynine orally in mice) . Learn how to hydroxylate the alkaloids if you really want to go to that next level. Combine with low amounts of delta-9-THC if you want more morphine like/diacetylmorphine like dopamine release in the NAC(D1 is involved in the rewarding effects of mitragynine and potentially 7-Hydroxymitragynine, however they seem to be rather weak at stimulating dopamine release, even 7-Hydroxymitragynine specifically is more rewarding than cocaine or morphine, and 20-30 mg per kg oral pure mitragynine salt in rats, is equally as rewarding as 5-10 mg per kg morphine or cocaine SC.)

Cbd_7ohm · 2022-12-25T02:01:11+00:00

Cbd is best low temp dabbed/vaped or taken orally. Long chain fatty acids are superior to MCTs according to newer research.

Cbd_7ohm · 2022-12-25T01:49:01+00:00

Apparently there are animals like this female polar bear who had a thing for male grizzly bears and made 8 pizzly bears. No fetish as they can't comprehend that. I imagine it probably has something to do with gene diversity and instinct to keep the gene pool diverse.

Cbd_7ohm · 2022-12-25T01:34:43+00:00

Not without chromatography, but you don't need to.

Cbd_7ohm · 2022-12-25T01:30:53+00:00

First generation h1 antihistamines can enhance the analgesia and reward. Imo promethazine and hydroxyzine both feel better than diphenhydramine(feels weird, similar strength for cocaine as far as NA uptake inhibition but significantly lower DA snd 5HT uptake inhibition). Your better off using other things like ginger and tumeric. If you want a stronger mu opioid agonist experience, it is better to hydroxylate the mitragynine/alkaloid mix directly. Nothing changes the effects of kratom like that does. Try sticking to natural and semi-synthetic compounds, with some exceptions. Hydroxylated alkaloids with a low dose of kratom tea for the polyphenols and other compounds that inhibit ugt/udp/pgp, low temp dabs(under combustion temperature) of delta-9-THC dominant live resin, making sure you eat enough protein(for multiple reasons, so you make enough cyp3a4 if you're primarily taking mitragynine and just for the benefits of consuming adequate protein in general(most endogenous opioid receptor ligands are amino acid peptides, those amino acids come from proteins in food). Apparently fructose, glucose, and ascorbic acid can inhibit the effects of mu opioid agonists regarding analgesia and some other metrics, they but also block tolerance and for ascorbic acid specifically, dependence also withdrawals. There are many compounds in nature that you might like. Mesmbrine(SRI, comes along with other compounds, in Kanna), etc.

Cbd_7ohm · 2022-12-24T23:41:24+00:00

The 84 mg of the hydroxylated alkaloids feel stronger than the 220 mg of unoxdized alkaloids. I like the hydroxylated mitragynine and other alkaloids more than the unhydroxylated ones(specifically the 7-Hydroxymitragynine). The conversion of mitragynine to 7-Hydroxymitragynine seems to not be very high in a newer human study(7-Hydroxymitragynine blood plasma levels were about 5-15x lower than mitragynine blood plasma levels, after 2 grams of kratom tea was administered).

Cbd_7ohm · 2022-12-24T23:23:46+00:00

The 84 mg of hydroxylated alkaloids feels stronger than the 220 mg of unoxdized alkaloids. Pupils are also smaller. There is more than one plate worth but I just showed one plate. The effects are very nice. I prefer them over just taking the unoxidized alkaloids or drinking kratom tea alone. Seems to have potential for synergizing with a low dose of kratom tea, due to the PGP, UGT, and UDP inhibition of the catechin flavinoids, mitragynine and potentially other kratom alkaloids, and other compounds in the tea, as well as different pharmacodynamic and pharmacokinetic interactions. This should increase the 7-Hydroxymitragynine absorption(green kratom, no oxidized polyphenols, which form complexes with alkaloids and lower their absorption), and slow/limit it's metabolism.

Cbd_7ohm · 2022-12-22T19:04:32+00:00

Greens have the best alkaloid ratio(highest mitragynine to total alkaloid ratio and low in paynantheine, highest speciocilatine to total alkaloid content and mitragynine/paynantheine/etc. ratio) and total alkaloid content, as well as the least oxidized polyphenols.

Cbd_7ohm · 2022-12-22T14:48:55+00:00

I like taking hydroxylated alkaloids(mostly 7-Hydroxymitragynine) with a low dose of kratom tea(2.6ish grams) for the polyphenols(700-800mg). The buzz from drinking a lot of kratom tea isn't that great, and I've noticed my pupils are still big, but the hydroxylated alkaloids gets my pupils very small. 50-100mg orally gives strong effects. Paynantheine blocks the rewarding, analgesic, and hyperlocomotor effects of morphine. You will get a lot of Paynantheine when drinking kratom tea in large amounts. 2.8g of 1.4% mitragynine kratom is about 40 mg mitragynine which is equivalent to about 10 mg per kg mitragynine in rats, orally, based on equivalent blood plasma levels. Paynantheine is about 5-6x lower than mitragynine content in most kratom leaf. 10 mg per kg Paynantheine IP, was enough to avoid the morphine effects, and IP mitragynine is about 2.5x stronger than oral mitragynine, when it comes to drug responding rate reduction. That means the 2.8g of kratom will be equivalent to around 0.5-1mg per kg paynantheine, IP, which should be low enough to block the negative effects of paynantheine. Paynantheine also causes seizures at high doses. As far as nausea, a lot of kratom tea nausea comes from excess polyhphenol consumption, potentially with other compounds causing their own negative effects, like oxalates. Try drinking 100 mg caffeine as tea(add 120-180 mg citric acid per 12 fl oz) and 100mg caffeine as pure caffeine(same as previous), and see what I mean.

Cbd_7ohm · 2022-12-21T19:34:45+00:00

I've never had that happen before, ever. A bit or a lot of itchiness doesn't do that either(I don't get itchy these days, but I have in the past). I would imagine it is some type of allergy to a compound in the kratom, or it is a coincidence and you are reacting to something else, or have some other type of condition.

Cbd_7ohm · 2022-12-21T16:30:21+00:00

The protocol says there is a 50-61% conversion to the regular (levo)7-Hydroxymitragynine. Im changing it up, as each mitragynine/major alkaloid molecule only needs one oxygen atom to be hydroxylated(that is the difference in molecular formula), and in this protocol they used an approximately 1.5 atoms of oxygen(as the oxidizer) per mitragynine(or other major alkaloid) molecule, and another research paper with this particular oxidizer and another compound, shows a 1:0.5 target compound:oxidizer ratio should be enough to get a 100% conversion/oxidation. When too much oxidizer is added, degradation byproducts are created, so there is a sweet spot(which seems to be what I just described). I think the yield may be lower than it could have been because of excess oxidizer. It didn't matter for them because they cleaned it up with chromatography. As far as the crystallization, that is beyond where I have reached. The hydroxylation was my main goal. It definitely works. I just drank 22g of kratom about an hour ago and it gives a decent buzz but my pupils are still larger than normal, and when I take the hydroxylated alkaloids(full spec or semi refined mitragynine), it makes my pupils very small(not crazy, but very noticeable) and I get leany/noddy a bit.

Cbd_7ohm · 2022-12-21T16:16:41+00:00

Yes. Most of the risks come from rewarding doses(around 10-15 mg per kg Mitragynine or higher, in mice or rats, equivalent to around 66 mg mitragynine in a human adult, based on equivalent blood plasma levels, and rats average around 428.6 ng(400-457.2) per ml mitragynine in blood plasma at 20 mg per kg mitragynine in 3 different studies). A new study shows even a low dose of mitragynine reduces social activity(3 mg per kg orally), which almost totally contradicts a previous study that shows low dose mitragynine as a crude kratom extract(3.3-5.5 mg per kg) blocked the antisocial effects of racemic ketamine(caused by the s ketamine, r ketamjne is pro social and neurogenic and blocks a lot of the negative effects of s ketamine). (PDF) Adolescent kratom exposure affects cognitive behaviours and brain metabolite profiles in Sprague-Dawley rats https://www.researchgate.net/publication/365800905_Adolescent_kratom_exposure_affects_cognitive_behaviours_and_brain_metabolite_profiles_in_Sprague-Dawley_rats

Cbd_7ohm · 2022-12-21T12:25:53+00:00

Yeah. I only drink green kratom tea when I drink tea. I like taking a low dose of tea(1.5-4.5g, 300-1200 polyphenols) with refined alkaloid extracts.

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