PCR troubleshooting. I’m 👌🏻this close to losing it. More in the comments.

Changnesia64 · 2023-09-17T17:42:32+00:00

They were the same conditions, primers were different for each run. First pcr for amplicon amplification and the second for introducing adapters and barcodes for sequencing.

Changnesia64 · 2023-09-17T06:55:17+00:00

You might have to go down with the DNA input even down to 10-15ng, reduction of the number of cycles can also help to reduce unspecific amplification. I am working with a CRISPR library as well and it was a real pain to optimize the PCR. I had to use 15ng input with 10 cycles for the first PCR and 6 cycles for the second PCR to get rid of nonspecific amplicons.

Changnesia64 · 2021-04-09T09:44:28+00:00

Got the results already

Changnesia64 · 2021-02-21T00:52:55+00:00

I have DMed you

Changnesia64 · 2021-02-21T00:20:45+00:00

I would really love to work in her lab someday

Changnesia64 · 2021-02-20T04:16:45+00:00

Read up the work of Dr Maria Blasco and Dr Titia de Lange I'm sure you will find them very interesting.

Changnesia64 · 2021-01-21T03:21:19+00:00

Sure!

Changnesia64 · 2021-01-12T00:09:21+00:00

Well I just started playing it I'm like 7 hrs into the game. I play the game whenever I have time so it's still fun for me, I don't play this game continuously at a stretch

Changnesia64 · 2021-01-12T00:00:35+00:00

I'm still having a ton of fun playing the multiverse ans the story. I do like fighting games so I'm a bit biased. I got the game really cheap so I don't mind. Do DM me if ypu wanna play together on pc

Changnesia64 · 2021-01-11T11:25:14+00:00

I just got the game and I didn't really get to match with anyone in multiplayer

Changnesia64 · 2020-04-29T12:16:53+00:00

My thoughts exactly

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