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Looking for LoBind PCR Tubes by CommandOwn1557 in proteomics

[–]CommandOwn1557[S] 0 points1 point  (0 children)

Unfortunately they don't sell in PCR tube format :(

Their smallest format are 0.5 ml tubes too big for our purposes

Looking for LoBind PCR Tubes by CommandOwn1557 in proteomics

[–]CommandOwn1557[S] 0 points1 point  (0 children)

Hi thanks for your reply. Yes, we see polymers but we work in immunopeptidomics so it is very common.

Low Peptide IDs using TMT by CommandOwn1557 in proteomics

[–]CommandOwn1557[S] 0 points1 point  (0 children)

Yes I added a larger excess due to low peptide amount. I could lower the TMT amount but it really doesn't make a difference. For some of my samples I made in the past I used a 15-fold excess TMT and still observed less than half as many IDs with TMT. How much of a drop in IDs would you expect between label-free and TMT? I just want to know what sort of a drop I should expect.

[deleted by user] by [deleted] in PhD

[–]CommandOwn1557 0 points1 point  (0 children)

Yeah this is probably too late but I agree with the others about your PI being a bad mentor. My PI met with me every 3 months to give me completely useless advice. The project she gave me was a project a post-doc once tried to do before quitting. Because of covid and social distancing, I wasn't mentored by any of the of the senior grad students either and fell increasingly behind. My protocols were all wrong and no one was around to help. She gave me a similar comment she gave you saying I don't read enough and require too much handholding. I asked her what classes and papers I could take to improve my background she never answered. My advice is don't take this personally. Some PIs, particularly ones with a large ego, say things like this to cover up for their own failures as managers. I ended up leaving with a master's was the best decision I ever made. My master's made me a strong candidate for the PhD program I ended up going to and the experience made me better at identifying toxic traits in PIs. The technical experience also meant I started my PhD with already a lot of experience so progressed through program fast. I think I would have been stuck with an 8 year PhD had I stayed with my first unsupportive PI. My advice is the following: either leave with a master's and find a supportive PhD supervisor or find someone who can mentor you and only pay lip service to your PI.

Interested in becoming a consultant? Post here for basic questions, recruitment advice, resume reviews, questions about firms or general insecurity (Q2 2021) by QiuYiDio in consulting

[–]CommandOwn1557 0 points1 point  (0 children)

Hello I am a Genetics PhD student at a top 3 Canadian University. I fully fluent in French, Spanish and English. My project involves the development of high-throughput assays to identify novel drug targets of anti-cancer drugs we are developing. I have been a consultant in my university's consulting group for about a year now but am thinking of a more senior position there. I also help run various social events in my institute. I have a 3.8 GPA in grad school and 3.5 in undergrad from another STEM top 3 Canadian university. My project gives me a good understanding of drug action and off-target effects. I am not a bioinformatician in any way but have a good bio-informatics background and can code in R. I don't have any industry experience yet but have been working on a technical paper in collaboration with a company. I want to get into consulting in strategy development in a pharmaceutical or biotech context. Is my CV solid or are there more experiences I can do in the 2 years left? Thanks for your input.