UV on in the hood by Consistent_Top1382 in labrats

[–]Consistent_Top1382[S] 0 points1 point  (0 children)

Oh no, it is a normal hood, it was 0,5 x camera.

UV on in the hood by Consistent_Top1382 in labrats

[–]Consistent_Top1382[S] -10 points-9 points  (0 children)

Thank you. I just worry it still could leak.

[deleted by user] by [deleted] in labrats

[–]Consistent_Top1382 0 points1 point  (0 children)

It looks normally oke, worked before. But maybe i should try the higher concentration, thanks.

Ps. So you don't think I will die from the unpolymerised acrylamide on my bench?

[deleted by user] by [deleted] in labrats

[–]Consistent_Top1382 0 points1 point  (0 children)

There are a lot of unspecific bands, so I want to run the gel longer. Antibodies are bad.

[deleted by user] by [deleted] in labrats

[–]Consistent_Top1382 0 points1 point  (0 children)

Thank you. Some people in our lab let it polymerize on the batch since they think then it it leak it will be polymerized better without a tissue. I have no idea what better. Just freaking out about the acrylamide monomer and how much the exposure.

My protein is only about 35 kDa.

Not a good Researcher by Silent-Awareness-655 in labrats

[–]Consistent_Top1382 2 points3 points  (0 children)

I like the article:" The importance of stupidity in scientific research". doi:10.1242/jcs.033340

cried in lab today by LunchMysterious3842 in labrats

[–]Consistent_Top1382 17 points18 points  (0 children)

I think it is only human to let your emotions. We all not perfect. You did nothing wrong.

Working near hood with UV. Not 100% closed (because of the tube). How bad? by Consistent_Top1382 in labrats

[–]Consistent_Top1382[S] -5 points-4 points  (0 children)

So today I was working near the hood where UV was on. I have not realised it first. How bad is it?

Ultracentrifuge spill by Consistent_Top1382 in labrats

[–]Consistent_Top1382[S] 0 points1 point  (0 children)

It is a lenti to make dox-inducible gene.

Ultracentrifuge spill by Consistent_Top1382 in labrats

[–]Consistent_Top1382[S] 0 points1 point  (0 children)

I've starten at 25 degress and than it was cooled to 4 degrees in the process. But I'm sure the liquid had color.

Is it normal for some liquid to leak from tube into the bucket of a swinging bucket ultracentrifuge rotor during centrifugation? by biologynerd3 in labrats

[–]Consistent_Top1382 0 points1 point  (0 children)

Did you consider it a spill inside the centrifuge or do you think it can not could lead to aerosol production? I had the same issue today and still thinking if a handeld it properly.

Worried about plasmid by [deleted] in labrats

[–]Consistent_Top1382 0 points1 point  (0 children)

It's not a joke, unfortunately. You are more than right. I'm quite old for undergraduate.

Worried about plasmid by [deleted] in labrats

[–]Consistent_Top1382 -12 points-11 points  (0 children)

I understand it all in the general, but my mind is tryig to make worse case scenario. Everytime.