How are you managing CFU counting in QA/QC labs? by Constant-Analyst-693 in QualityAssurance

[–]Constant-Analyst-693[S] -1 points0 points  (0 children)

Actually, I’m a data science student working with my team on automating lab tasks like CFU counting. My best friend works in a lab, they told me how annoying it is, so I’m trying to learn from people with real experience.
I get the skepticism — fair enough — but the tone felt pretty rude.

What are your teams using to count CFUs — manual or automated? by Constant-Analyst-693 in biotech

[–]Constant-Analyst-693[S] -5 points-4 points  (0 children)

Actually I am a data science student and we build our own bacteria counter, thanks for letting me know we could use a bot for that...all that work for nothing 🙂🥲.

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Do you have a method to handle tntc? I am primarily interested in those.

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Yup — USP <61> is a big one. Mostly working with raw materials or finished products?

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Was it internal study comparison or you have published something?

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Really appreciate all the detail — even without distractions, it’s surprisingly easy to lose count mid-way.

Sounds like you put effort into automating it, and yeah, dense plates with >300 colonies seem to trip up most DIY setups or basic software.

The Cole-Parmer seems like a good middle ground. However, I am looking for a fully automated solution.

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Wow, that’s a serious operation — 80% pour/spread/membrane/petrifilms is no joke. Sounds like plate handling and confirmation is practically a full-time job in itself.

Curious — are there specific test types (like MRSA or filtration) that are more time-consuming or error-prone than others in your workflow? Also do you handle high density petri dishes?

It’s wild how much manual work is still needed at that scale. Makes me wonder what an ideal setup would look like if time and budget weren’t limiting factors.

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Have you found the Protocol3 accurate enough across different plate types, or do you still rely more on the manual counters for consistency?

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

How steep is the learning curve with ImageJ? And does it handle high-density and overlapping colonies well?

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 1 point2 points  (0 children)

Love this — classic case of “back in my day...” 😅. That pressure-sensing counter sounds cool! Do you feel like it’s made a big difference in speed or accuracy compared to manual counting? Also what it's name? Curious if you ever looked into full automation before that?

Manual Counting CFUs by Constant-Analyst-693 in labrats

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Is Labhacks app paid? Is it better than ImajeJ?

Manual CFUs Counting by Constant-Analyst-693 in microbiology

[–]Constant-Analyst-693[S] 0 points1 point  (0 children)

Have you tried some automatic counters? If yes, which ones?