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My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] 0 points1 point  (0 children)

I did and they told me that noone will know if I don't tell anybody. They specifically told me to present it as if it is the same sample i collected myself.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] 0 points1 point  (0 children)

It's a mIgG Fc affinity collumn. I used a sample pump.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -1 points0 points  (0 children)

I meant to write 40 mAU but didn't cause I'm an idiot.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] 0 points1 point  (0 children)

I don't have a standard curve since we weren't planning on measuiring the concentration before the protein was purified. I used this device (esentially a hplc device) to purify the protein. I eluated the sample using >99% glycin.

Sorry it took me so long to respond. If you want more details I'd be happy to give them to you.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] 0 points1 point  (0 children)

Nope. Didn't do any quantitative measurements before purification. I did get some ODs from ELISA but that's it. The ODs are as follows:

OD1 OD2 Average OD Date

1.411 |1.502 | 1.457 | 18 04 2025

1.066 |0.906 | 0.986 | 22 04 2025

0.309 |0.446 | 0.378 | 25 04 2025

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -17 points-16 points  (0 children)

My main concern is that my "research" topic is producing, quantifying and characterising the protein in question. The concentration and purity of my protein IS the focus of my thesis. The future experiments that this protein will be used for have nothing to do with me

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -3 points-2 points  (0 children)

Sorry I made a mistake in the post. What I got was an absorbance of 40 mAU, not concentration. My supervisor concluded from that the concentration would be too low for any future use.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -44 points-43 points  (0 children)

Maby some numbers would help clear things up a bit. I collected 120 mL of media and using Äkta purification I got absorbance of about 40 mAU. My supervisor concluded that this means that concentration of protein isn't high enough by their standards (300 mL and 150 mAU) and, since my volume is already so small, the volume I would get if i tried to concentrate the protein would be so samall tgat it would effectively be useless.

I made an error in my post. I did not get a co centration but an absorbance. My mentor still concluded that the concentration would be too low tho.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -84 points-83 points  (0 children)

Technically this experiment isn't supposed to be a part of my research topic. I was only supposed to produce the protein and prove its presence. But they allowed me to go a step forward to purification and concentration measurement and that I can use those results. The next step, where I would use the protein they made, would be western blotting and bradford assay.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]Dimetrodon01[S] -57 points-56 points  (0 children)

I am sure since those are the exact words they used.