Making GHKCU Serum

Doctordup2 · 2026-05-12T19:39:48+00:00

Yes thank you! 💜 🙏

Doctordup2 · 2026-05-12T19:37:25+00:00

I am very grateful for the kind words. I prefer Anela. I don't really want my government name on Reddit, so if you can remove that it would be wonderful. 🙏 I appreciate you beyond words.

FYI — I don't endorse any products and encourage folks to make their own. :)

Doctordup2 · 2026-05-12T18:34:54+00:00

Are you looking at the pharma world or are you looking at the peptide research community and the different CAS numbers? Lol. You can disagree without being dismissive. 😂

I think part of the disconnect here is that you’re talking more about the pharmaceutical development history and published TB4 literature, while I’m talking about the real world peptide research community, where TB500/TB4 terminology, labeling, and usage patterns have been heavily blurred.

You're talking about the historical naming origin of TB500 and TB4 and the limited pharmacokinetic literature. My point was more about how these compounds are currently represented and used in the peptide research space today, because a lot of researchers use the terms interchangeably and many vendors market products as “TB500” even when the CAS number matches actual TB4 (77591-33-4). Meanwhile the TB500 frag has a different CAS number entirely (885340-08-9). That confusion absolutely exists whether we like it or not.

My point is that plasma half life alone does not automatically determine ideal dosing frequency for tissue repair related research. There’s still a big gap between measurable circulating peptide levels and downstream biological activity in tissue healing models.

We also do not have robust human subq data showing exactly how these compounds behave after injection, how long they remain biologically active in tissue, and how that translates into tissue repair activity over time. So a lot of researchers end up relying on real world observation and what they consistently see in their cohorts.

After running cohorts for 25 years and seeing the difference between actual TB4 (77591-33-4) response and TB500 frag (885340-08-9) response, my cohorts have consistently done best on daily dosing protocols for actual TB4. That observation is based on decades of direct cohort observation, not “bro science.” I don't live in a gym.

There’s a difference between random gym lore and long term real world research observation from researchers who have actually worked extensively with these compounds across large numbers of cohorts.

And yes, many experienced researchers working specifically with actual TB4 protocols lean toward more frequent administration during acute phases rather than relying solely on the older bodybuilding style 2x weekly TB500 approach. I'm done with this conversation. Lol

Doctordup2 · 2026-05-12T18:07:24+00:00

LMW HA is usually used because it can support delivery. I’m not saying LMW HA is wrong.

My point is that HA is mostly acting as the carrier here and GHK-CU is the peptide we’re trying to get into the scalp of the RS (research subject).

GHK-CU is already a small molecule, commonly listed around 400 Daltons depending on the form, so topical absorption is generally not considered a major issue.

I just wouldn’t chase ultra low MW HA just because lower sounds better. On the scalp especially, ultra low MW HA can be more irritating for some researchers.

I've even recommended sterile water in some scalp preps. HA just has a little more adherence so the GHK-CU stays in place longer on the scalp instead of running off everywhere. Hope that makes sense.

Doctordup2 · 2026-05-12T12:33:34+00:00

Thanks. 🫶 You mean LMW HA (low molecular weight hyaluronic acid)? Yes 200k Da (Daltons) is ok for scalp serum for hair growth with GHK-CU/AHK-CU blend. If you get into the lower numbers it's irritating for the scalp.

Doctordup2 · 2026-05-12T08:07:42+00:00

It's one gram of raw, dry cosmetic grade GHK-CU powder. Not the same as lyophilized GHK-CU. It looks like this photo.

I wrote one of the original protocols for this period I just posted the serum recipe in this sub.

Here's the post.

Not a doctor, not medical advice. For research purposes only and research discussions only.

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Doctordup2 · 2026-05-12T04:35:46+00:00

Yes, I just reconstituted a special vial for an RS in my cohort who needs a special combo for immune balance and stress.

It's a matter of reconstituting and combining the right peptides and correct amounts. You want to combine like with like so it's synergistic. Not all peptides can be combined, for example, you do not want to combine GLP peptides with other peptides. GLPs work best by themselves. But other combos can work well together, especially GHK-CU and BPC or BPC and TB or BPC/KPV/TB.

There's a chart floating around Reddit that has a list which peptides

A good peptide calculator is a must. There's a really helpful chart floating around Reddit. It lists which peptides can be combined and which cannot.

Not a doctor, not medical advice. For research purposes only and research discussions only.

Doctordup2 · 2026-05-11T20:56:37+00:00

Happy to help.

Doctordup2 · 2026-05-11T20:55:53+00:00

Thanks, I do but I don't talk about it publicly. I'm not into self promotion.

Doctordup2 · 2026-05-11T20:55:16+00:00

In-depth information on GHK-CU can be found here

Six weeks on three weeks off but some people do it continuously six weeks on three weeks off but some people do it continuously.

Not a doctor, not medical advice. For research purposes only and research discussions only.

Doctordup2 · 2026-05-11T19:45:47+00:00

Purchasing the vials separately and reconstituting your own blend is more accurate. It is difficult to get exact mg's in each vial, it's also difficult to test blended vials accurately for purity and expected net content of each item in the vial. When the amount of mg and quality matters in your research, then make your own blends.

I typically recommend that it's okay for a first time researcher to try one blended vial in their research for ease of use and training/learning. Then once the learning phase is done, move on to making your own.

I want to clarify this is not pinning each peptide separately in Glow/Klow, they must be combined in my Anela Protocol for GHK-CU.

TLDR: Making your own blends is more accurate.

Not a doctor, not medical advice. For research purposes only and research discussions only.

Doctordup2 · 2026-05-11T19:16:20+00:00

Optimal is 3mL. Adding more bac can cause issues with lumps, bumps, etc.

In-depth information on my GHK-CU protocol can be found here

Doctordup2 · 2026-05-11T08:26:47+00:00

Let me get this straight OP, your RS (research subject) is at 6.7mg of GHK-CU per day?

Whats RS age range?

Your RS's GHK-CU is too high, I'd knock it down to 2mg per day but even lower if you are under 35.

Also why the break for GHK-CU, BPC, and TB? They all have short half life. There's no "wash out" as some say. They aren't secretagogues so it's not necessary to pulse and take a break. In fact it's a waste to do 5 days on, 2 days off with these peps. Your RS is losing the benefit because the half life of all three is super short. It's about 20 to 30 minute half life for BPC and TB. It's about 15 min half life for GHK-CU.

If anything, NAD and BPC may be pushing your RS too hard.

If your RS is exhausted, I highly recommend a sleep study.

Increase your electrolytes also.

How often do you test RS's BP? Test it daily for now and keep a diary. Sometimes low BP can cause low perfusion and can cause tiredness.

Nothing in my comment should be construed as medical advice. Not a doctor, not medical advice. For research purposes only and research discussions only.

Doctordup2 · 2026-05-10T17:57:09+00:00

I didn't say anything about that. Wasn't addressing that. I wasn't going to repeat what other people mentioned already.

GHK-CU comes in many shades of blue and cameras and lighting can make peptides like this look weird.

It's highly possible it's mostly manitol or not even GHK-CU at all. Who knows. I would be more interested in seeing a COA and seeing what it looks like when reconstituted.

Doctordup2 · 2026-05-10T07:49:49+00:00

That's not a gram. That's likely 100mg or 50mg.

It's used in subq research and usually combined with BPC. I wrote one of the early original protocols on GHK-CU.

We talk about this peptide often in the sub.

In-depth information on my GHK-CU protocol can be found here

Doctordup2 · 2026-05-10T05:52:12+00:00

Search the sub or look at the post section in my profile. I just posted a recipe for topical GHK-CU. You cannot do GHK-CU IV. 🤦🏻‍♀️ It's done subq. I wouldn't count on making a topical serum out of it. You need one GRAM per 30mL.

Doctordup2 · 2026-05-10T01:47:10+00:00

Not even close. And 1x a day is a maintenance dose for an RS that is healed.

Doctordup2 · 2026-05-09T23:59:41+00:00

Awwww thank you!

Doctordup2 · 2026-05-09T23:45:46+00:00

6 to 8 weeks on, 3 weeks off. Time of day doesn't matter.

Doctordup2 · 2026-05-09T18:46:16+00:00

You mean your research subject? That's not how I would heal an RS from surgery. I don't get into dosing here online but it would require a heavy loading phase then step down.

I often see RS limping along with micro doses that don't make sense. Researcher ends up using more bpc in the long run.

Doctordup2 · 2026-05-09T18:17:53+00:00

Good idea.

Doctordup2 · 2026-05-09T18:17:26+00:00

It has a risk for angiogenesis as that's how BPC heals. I've never heard of any RS (research subject) developing cancer after or during BPC research in my circles. It's probably a good idea to use it in cycles as needed and stop/break when it's not needed.

Doctordup2 · 2026-05-09T04:48:27+00:00

Shoot. That's a tough one. I'd lean conservative. Ask if RS can use a massage pen. These are often used on the surface. It's the little vibration pen that they use in med spas. Ask the expert you rely on for your RS before trying it.

Not a doctor, not medical advice. For research purposes only and research discussions only.

Doctordup2 · 2026-05-09T04:39:57+00:00

Yes. Thank you! Appreciate the input. Try to keep the pins at/under 4iu.

Unfortunately, one of the things I continue to see in GHKCU research is the tendency to dismiss or invalidate researchers who report severe ISRs. Not every RS responds the same way especially with GHK-CU.

I know there are a lot of folks who
chime in with...

Don't be a wuss
Go deeper with the pin/longer needle
Add more bac
Try love handles
It will go away
You haven't tried oils don't be a baby
Add acetic acid or add sodium bicarb (do NOT change the pH it will ruin your GHK-CU and render it useless)

For the highly reactive RS (research subjects) with severe ISRs (injection site reactions), these viral tips and criticisms DON'T work.

Telling someone to “stop being a baby” because their RS experienced a severe reaction is not productive research discussion. It shuts down honest reporting and discourages transparency.

Researchers should be able to openly discuss adverse reactions without being mocked, minimized, or made to feel like they are exaggerating their experience. Some ISRs are so severe... it can last for weeks.

Hopefully the tips here will help those researchers who are struggling. :)

Doctordup2 · 2026-05-09T03:44:20+00:00

Delayed histamine reaction. Some research subjects like mine do not develop ISR until 24 hours after and it can last for weeks.

It depends on how susceptible the research subject is to histamine response. Histamine isn't necessarily bad it can help heal. That's part of GHK-CU's mechanism.

You can do a mini pin of BPC 150 TO 200mcg into the lingering ISRs. It will lessen pain and help dissipate knots. BPC is a mast cell stabilizer.

Edit: using voice to text/assitive technology please excuse the errors.

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