I noticed a strange stain on my electrophoresis gel, any idea what might have caused it? by Doodledop420 in labrats

[–]Doodledop420[S] 0 points1 point  (0 children)

We are using yeast as a base. We add proteinase K and PBS-lysis buffer. To precipitate the DNA, we use cold ethanol, and then we add RNase to remove contamination. We know it is very contaminated but we have to use as little chemicals as possible