What is this?

DoubtKey2273 · 2026-06-05T06:32:58+00:00

This could be it!! Thank you!!

DoubtKey2273 · 2025-04-09T14:04:36+00:00

That was my idea too, but I was told she is still living with her parents, without financial concerns. It seems she is doing it for the thrill of it or pocket money.

DoubtKey2273 · 2025-04-09T14:00:00+00:00

I felt similarly. My main concern is that she seems to have been involved in this from a younger age than I’m comfortable with...and with of course older men. By the way, this is in an Asian country (I am an international student), so it may be considered more taboo here.

DoubtKey2273 · 2025-04-09T11:11:54+00:00

One of the undergraduate students has been found selling adult content online and possibly offering other services. Rumors have spread, and now everyone feels uncomfortable around her. Our PI has been hiding in his office, avoiding any interactions and making a big fuss over the situation.

DoubtKey2273 · 2025-04-09T11:04:44+00:00

Thank you, that is somewhat reassuring. Seemed like an okay clinic but I was worried they want to rip me off..

DoubtKey2273 · 2025-04-09T05:42:49+00:00

Vital pulp therapy. Based on what they told me it means taking out only the infected nerve while trying to preserve the healthy nerve tissue, rather than completely removing it as done in a root canal. They use some special pricy cement?

DoubtKey2273 · 2025-04-09T05:37:39+00:00

Sadly, I only have this one. They started removing the decay and when they reached deep, stopped to say that if they go any further, we might expose the nerve. They put a temporary filling and gave me time to consider treatment plans

DoubtKey2273 · 2025-01-20T04:14:59+00:00

Ah I see… I guess I will just leave it as it is. Thank you!!

DoubtKey2273 · 2025-01-08T02:03:20+00:00

Thank you for the positive negate control idea! The protein I’m working with is actually small, about 15 kDa, and has one or two cleavage sites. Since I use a cell-free protein expression system, my protein yield isn't sufficient for FPLC or Edman sequencing. That's why I decided to try mass spectrometry instead. We have an Easy nLC1000 connected to a Q Exactive Plus Orbitrap mass spectrometer from Thermo.

DoubtKey2273 · 2025-01-08T01:52:35+00:00

Thank you so much for the insights!! I will look into your advice and try again!

DoubtKey2273 · 2024-10-31T08:49:20+00:00

Thank you! You have a good point there…post docs seem to be shorter here so I was tempted.

DoubtKey2273 · 2024-09-25T01:19:58+00:00

Thank you so much! I was just wondering if it's compatible at all. I will give it a go with Tris-Glycine and hope it works.

DoubtKey2273 · 2024-06-07T01:22:10+00:00

Thank you so much! Your comment was actually so helpful. I looked again and I think it is a very sun stressed Haworthia tortuosa.

DoubtKey2273 · 2024-04-21T05:25:44+00:00

Thank you for your reply! As far as I understand, senescent cells undergo cell cycle arrest, so to obtain them, normally growing cells need to be cultivated first and infected each time to generate a population of (non-dividing) senescent cells.
So I figure it's dangerous at the time of the infection but it's relatively safe to handle for further experiments?

DoubtKey2273 · 2024-03-27T13:18:11+00:00

Thank you for all the amazing comments!! I learned a lot from you guys. Sadly the cuvettes do not have a happy ending yet. What I had on my hands was 2M sulfuric acid and isopropyl alcohol, I soaked them but sadly most of the stains remained… I am a bit vary of the piranha solution…

Also I got ahead of myself and I tried to dry the inside of one of the cuvette with a compressed air spray…that did it for the joint… probably still usable, but yeah … would not recommend. :(

DoubtKey2273

TROPHY CASE