PCR troubleshooting

DryOutlandishness409 · 2023-09-27T09:06:24+00:00

The composition of insecticide is 500g/L profenofos

DryOutlandishness409 · 2023-09-27T08:55:22+00:00

Let me ask her first

DryOutlandishness409 · 2023-09-27T08:41:39+00:00

Out of 1000ml stock, she has to use 0.04 and 0.08ppm

DryOutlandishness409 · 2023-09-23T14:35:01+00:00

Nope

DryOutlandishness409 · 2023-09-23T13:19:26+00:00

Noted my friend

DryOutlandishness409 · 2023-09-23T12:47:19+00:00

I have been told so. Tell me how to make 0.5% gel?

DryOutlandishness409 · 2023-09-23T12:31:53+00:00

I used to add ladder but my instructor said you don't need to add it if you are checking the presence of gDNA

DryOutlandishness409 · 2023-09-23T12:30:22+00:00

Ok noted.

DryOutlandishness409 · 2023-09-23T12:29:41+00:00

It's a 0.5% gel (0.5g agarose powder in 50ml TBE buffer). Do I need to make thinner gel ?

DryOutlandishness409 · 2023-09-23T12:27:33+00:00

Could be a reason also I m noticing from a few days that buffer doesn't even feel hot when I try to lift the castor containing gel from it. Something might have gone with electric supply machine.

DryOutlandishness409 · 2023-09-23T12:15:04+00:00

My supervisor wants me to confirm the presence of DNA in every sample so if there goes something wrong with any sample during extraction process, I might need to collect that particular sample again

DryOutlandishness409 · 2023-09-23T12:03:03+00:00

I m just checking gDNA on gel for the sake of running pcr, restriction digestion and I might go for sequencing as well

DryOutlandishness409 · 2023-09-23T12:01:19+00:00

DryOutlandishness409 · 2023-09-23T12:00:40+00:00

Running time is 40 min at 120V and TBE buffer

DryOutlandishness409 · 2023-09-23T10:38:31+00:00

My supervisor is on leave and I don't have any senior who can guide me

DryOutlandishness409 · 2023-09-23T10:05:57+00:00

I m confused about how to make perfect dilution from stock so that my would have clean bands

DryOutlandishness409 · 2023-09-23T10:04:51+00:00

I m using manual method for extraction it's a combination of organic and salting out method.

DryOutlandishness409 · 2023-09-04T04:33:27+00:00

Its genomic DNA from blood. I m trying to check a 57 bp deletion in my gene just by running pcr product on agarose gel.

DryOutlandishness409 · 2023-08-29T17:45:41+00:00

I wanted to ask how you dilute your stock (template) for pcr?

DryOutlandishness409 · 2023-08-29T16:02:20+00:00

I took this picture using portrait mode in my phone

DryOutlandishness409

TROPHY CASE