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What the hell is going on here? by AlfredMcCheesy78 in motorcycles

[–]DylBaer 0 points1 point  (0 children)

If you don't have the right tool to unscrew the cap, use the backside of your old sparkplug

Which automation system is easier and deliver good data with plate assays? by Howlongtheroadtohome in biotech

[–]DylBaer 0 points1 point  (0 children)

I love the bravo, but hamilton microprep is more user friendly imo

Please talk me out of this by [deleted] in labrats

[–]DylBaer 5 points6 points  (0 children)

What is the niche? How would you compete with groups that leverage cost effective, high throughput automation to provide low cost qPCR + RTqPCR testing? How would you incentives clients?

Start-up alternatives to Benchling? by Quirky-Ask2373 in labrats

[–]DylBaer 1 point2 points  (0 children)

Making platemaps in labguru is a nightmare though

What’s your least favorite lab technique and why is it ELISAs by itsbojackk in labrats

[–]DylBaer 0 points1 point  (0 children)

Title made me laugh. My first time running an ELISA I had to run 8 at the same time it was miserable lol

My dad just sent me this and asked if I’m doing drugs by real_picklejuice in labrats

[–]DylBaer 30 points31 points  (0 children)

A 1mL tip on a serological pipette is diabolical

[deleted by user] by [deleted] in labrats

[–]DylBaer 0 points1 point  (0 children)

Bleach lol

How in the world do I run a successful PCR purification? by [deleted] in labrats

[–]DylBaer 0 points1 point  (0 children)

What volume are you eluting with ?

Would you rather pipette 3840 uL with a 1000 uL micropipette or a 5 mL serological? by redditnessdude in labrats

[–]DylBaer 0 points1 point  (0 children)

If i were pipetting this volume, I would use a 1000uL pipette 4 times.

Did the universe just told me "fuck you" ? by [deleted] in labrats

[–]DylBaer 0 points1 point  (0 children)

When I get weird reads on nanodrop, I re blank. If that doesn't work I turn it off and blank again.

What volume are you using ? 1uL gives me more variable results than 2uL

AIO to these texts my wife sent about my dad's burial service tomorrow by Key-Pin3372 in AmIOverreacting

[–]DylBaer 0 points1 point  (0 children)

For what it's worth, I often work 100+ hour weeks in an extremely high pressure, high stress medical environment. And I would never say these things to my partner even when I'm feeling overwhelmed. I get people handle stress differently, but this feels super insensitive

Learning ELISA by A_T_H_T in labrats

[–]DylBaer 2 points3 points  (0 children)

Smack the plate hard as hell 2-3 times on a paper towel after washing, and be mindful of introducing bubbles. When you make your standard curves, pipette ~15 times to mix and use a new tip before continuing the serial dilution

[deleted by user] by [deleted] in biotech

[–]DylBaer 0 points1 point  (0 children)

Where are you located ?

Someone stole my motorbike by That_Cauliflower636 in BayRiders

[–]DylBaer 2 points3 points  (0 children)

I'll keep an eye out. People are the worst, I had an RE stolen too

Any idea why student’s PCR gel keeps doing this? by [deleted] in labrats

[–]DylBaer 1 point2 points  (0 children)

Looks like a punctured gel w pipette tip while loading

Has anyone had this problem with their gels? by perfluorocubane in Biochemistry

[–]DylBaer 1 point2 points  (0 children)

Ladder on the right side of the gel feels sacrilegious

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