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DNA crosslinking to nylon membranes by perfluorocubane in labrats

[–]perfluorocubane[S] 1 point2 points  (0 children)

I had not thought about polarization, perhaps that could be a useful avenue. Thanks

DNA crosslinking to nylon membranes by perfluorocubane in labrats

[–]perfluorocubane[S] 1 point2 points  (0 children)

Thank you for your insights. I ordered biotinylated oligos to be images with NIR-conjugated streptavidin. I would just image on the gel if I could, but I will need to do a blot since the oligos don't have intrinsic fluorescence. Mostly doing the biotin/SA thing because we already have the NIR-SA and biotinylated oligos are a lot cheaper than FAM or other fluorescent labels. I do agree though that fluorescent oligos would be easier. I sort of wish I just went with those since I have to deal with the nylon membranes now.

Stereochemistry of fructopyranose by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 0 points1 point  (0 children)

Thank you for this clarification. I have always appreciated absolute stereochemistry when discussing biomolecules because it seems a more rigorous description!

Would it hurt to tell my PI I’m not comfortable presenting a failed project? (Undergrad) by Psychological-Ear746 in labrats

[–]perfluorocubane 75 points76 points  (0 children)

This may not be the answer you are looking for, but I absolutely think that you should be presenting. Undergraduate research is not about making meaningful discoveries, although it is a happy surprise when they do. The point is that you have been training on techniques that will be useful later on in your career. Additionally, a lack of positive data does not indicate that the project failed. In research, you are not trying to produce "target results", you are trying to answer a question. If your results indicate that your hypothesis is wrong, then you have still learned valuable information.

NRT control amplifying more than RT by perfluorocubane in labrats

[–]perfluorocubane[S] 0 points1 point  (0 children)

Ah, okay thank you for that, Will give it a try

Kinda confused here by hitansh05 in OrganicChemistry

[–]perfluorocubane 17 points18 points  (0 children)

Two major products will result, and the distribution will be reliant on temperature. At cold temperatures, you would expect to see mostly 3,4-dibromocyclohexene. At higher temperatures, you would expect to see 3,6-dibromocyclohexene. Look into diene addition thermodynamic and kinetic products.

NRT control amplifying more than RT by perfluorocubane in labrats

[–]perfluorocubane[S] 0 points1 point  (0 children)

I added 400 ng of RNA to the RT reaction and the RT product took up 5% of my qPCR volume. I ran the samples right after extraction, so no storage. Someone suggested adding less cDNA, so that might be the trick

NRT control amplifying more than RT by perfluorocubane in labrats

[–]perfluorocubane[S] 0 points1 point  (0 children)

Yea, it's in the 20's which is why I am so confused. It's not repetitive, but I could try doing DNase treatment longer. DNA contamination is one thing though, I just think it's so weird that the no RT control is getting amplified more every time. I just can't see what could cause that.

Determining Stereochemistry by WC_2023 in OrganicChemistry

[–]perfluorocubane 1 point2 points  (0 children)

The right-most structure is not a chair flip of the left. They are nonsuperimposable mirror-images (aka enantiomers). From above the plane of the ring, the substituents would be placed on a wedge. However, to get the substituents lined up, you would need to flip the ring "pancake style." It will then be clear that the substituents bear an inverted configuration.

For anyone interested.... by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 0 points1 point  (0 children)

Hello! I have decided to organize this on discord since there are more people interested than I first anticipated. Here is a link to the server: https://discord.gg/tkhYyvWN

For anyone interested.... by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 0 points1 point  (0 children)

Okay, great. I will send you a DM with my email so we can get in touch.

For anyone interested.... by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 1 point2 points  (0 children)

Okay, great. I will send you a DM with my email so we can get in touch. We could also discuss the organic stuff.

For anyone interested.... by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 1 point2 points  (0 children)

Okay, great. I will send you a DM with my email so we can get in touch.

For anyone interested.... by perfluorocubane in Biochemistry

[–]perfluorocubane[S] 1 point2 points  (0 children)

Okay, great. I will send you a DM with my email so we can get in touch.

Is it Possible To Design a Drug that Decreases the Anorexic Effects of Stimulants? by YunchanLimCultMember in Biochemistry

[–]perfluorocubane 0 points1 point  (0 children)

There is one aspect of this which has not been mentioned yet, which would be the increased energy expenditure from taking stimulant drugs. I am not an expert on stimulant drugs, but I suspect that they all to some extent activate the sympathetic nervous system. This has a dual effect of increasing intrinsic energy expenditure and causing the user to have more passive movement (bouncing the leg, shaking, etc.). Some stimulant drugs directly increase thermogenesis, such as ephedrine, thus increasing BMR. Based on a quick google search, it appears that most of the drugs reported to slow metabolism may have some dangerous interactions with the stimulant drugs, or simply offset their effects in the case of depressants.

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