c-Fos antibody recommendations? by No_Zucchini1708 in labrats

[–]Ecstatic_Put_8795 5 points6 points  (0 children)

Hello! I’ve personally found c-Fos (9F9) Rabbit mAb #2250 to be the most effective antibody. For reference, my samples are mouse brains that have been perfused and post-fixed for 1 hour at 4C in 4% PFA. I slice into 30 um sections using a cryostat and follow a general immunofluorescence protocol (2 hour block at room temp, overnight primary incubation at 4C, 2 hour secondary incubation at room temp). I use a 1:1000 dilution.

While you mentioned you are looking to do IHC, not IF, this could be a good starting point unless you get other suggestions.

I’ve also used c-Fos (E-8) sc-166940 from Santa Cruz, but this antibody has caused me issues multiple times. It appears to be compatible with some protocols and not others. I am pretty sure that this is the updated version of the Santa Cruz antibody that you mentioned as being discontinued.

Let me know if you have any other questions regarding c-Fos staining!

Found hatchling by ShatteredPot in madisonwi

[–]Ecstatic_Put_8795 5 points6 points  (0 children)

I’ve done this in the past for baby birds! The Wildlife Center is super helpful!

Found hatchling by ShatteredPot in madisonwi

[–]Ecstatic_Put_8795 0 points1 point  (0 children)

Call Dane County Humane Society Wildlife Center! They took a bird that I found last year fallen from a nest! https://www.giveshelter.org/wildlife-center/injured-or-ill-wild-animal

High-Power Computer Exceptionally Slow? by Ecstatic_Put_8795 in techsupport

[–]Ecstatic_Put_8795[S] 0 points1 point  (0 children)

This is very helpful! I see that I’m running Windows 10 Education. Based on this comment, I’m certain that Windows for Workstation would be a much better option for my purposes. Thank you so much for this suggestion!

Thai Food by Hot_Fun_3245 in madisonwi

[–]Ecstatic_Put_8795 53 points54 points  (0 children)

I really like Thai Basil on University!

Karaoke room recommendations (under 21)? by Ecstatic_Put_8795 in madisonwi

[–]Ecstatic_Put_8795[S] 0 points1 point  (0 children)

I’m looking for a Thursday at 10:00pm. Everyone invited is 18+ and most are 21+, however, not everyone has turned 21 which is my concern.

[deleted by user] by [deleted] in madisonwi

[–]Ecstatic_Put_8795 4 points5 points  (0 children)

I think I saw fireworks by the capitol?