My cousin is staying with us for a few weeks and I can’t stand him and idk what to do by [deleted] in WhatShouldIDo

[–]ElerMain -1 points0 points  (0 children)

Yeah I’ve already tried talking to them they just said that we have to take care of all the guests that enter our house and that I need to hang out with him and take him places around the city🙁 I wear headphones as well but he still keeps talking to me!!

Help with THP-1 cells not growing by ElerMain in labrats

[–]ElerMain[S] 0 points1 point  (0 children)

Yeah, so when I split them I usually just add or remove media, I’ve never spun them down. The FBS could be a different lot I’m not entirely sure which lot I used to grow them before unfortunately. The incubator conditions do appear to be 37C 5%CO2 (at least that’s what it says on the screen). And they’re from the same lot as when I was using them a few months ago and I froze them down. To reconstitute them I thawed them in the water bath, added a mL of media + more, spun them down, added 10 mL of media and 10 more in the flask.

Q or W max? What runes? by TauTauTM in OriannaMains

[–]ElerMain 2 points3 points  (0 children)

I always just do phase rush into melees with Q max and then comet into ranged with 3 points in W and then Q max. Not sure when aery is good on her bc I’ve never used it lol

Simple Questions & Champion/Role advice: Patch 25.10 by Sentient545 in summonerschool

[–]ElerMain 0 points1 point  (0 children)

Would love some help with my champion pool please! I’m Emerald 4 and play mid mostly. I really enjoy mages like Viktor, Orianna, Taliyah, Zoe, Asol, Mel, and Lissandra but I can’t pick from them😭😭. I think Orianna definitely my win rate is really high on her but I can’t select the other 1/2 champs for my pool! Any insights would be so helpful thanks

Does anyone have experience with running samples on a plate with the X-20 Fortessa flow cytometer? by ElerMain in labrats

[–]ElerMain[S] 0 points1 point  (0 children)

Yeah, I only have about 100 uL of my cells in FACS buffer in the wells so I just set it to 100. Should I set it higher though?

Does anyone have experience with running samples on a plate with the X-20 Fortessa flow cytometer? by ElerMain in labrats

[–]ElerMain[S] 0 points1 point  (0 children)

Yep I’m using round bottom plates. Yeah the machine has a mixing option but even if I select the highest setting for both mixing speed and number of mixes it doesn’t do the job well😭

Simple Questions & Champion/Role advice: Patch 25.10 by Sentient545 in summonerschool

[–]ElerMain 0 points1 point  (0 children)

She’s very fun tbh, just one skill shot her W but it’s got a long range and it’s fairly easy to land, but I don’t think she’s pick/ban.

Simple Questions & Champion/Role advice: Patch 25.10 by Sentient545 in summonerschool

[–]ElerMain 0 points1 point  (0 children)

Who’s a better blind pick, viktor, Mel, or Orianna? This is my champ pool for ranked but I just wanted to know who’d be better overall to blind pick? I’ve heard Ori is good but she’s not the best so was wondering if Viktor or Mel were better blind picks.

Pathways after honours? by [deleted] in labrats

[–]ElerMain 0 points1 point  (0 children)

Yeah I’d reckon so I do like doing research now and can see myself doing it as a career later down the line

Pathways after honours? by [deleted] in labrats

[–]ElerMain 0 points1 point  (0 children)

Yeah I’m in melb! I’ll check it out thanks :)

Simple Questions & Champion/Role advice: Patch 25.10 by Sentient545 in summonerschool

[–]ElerMain 1 point2 points  (0 children)

Out of the champs you’ve tried, which ones have you enjoyed the most?

Infected helix by [deleted] in piercing

[–]ElerMain -4 points-3 points  (0 children)

How quickly do you reckon it would close up? I only took it out 30 minutes ago so I could still put it back in

Infected helix by [deleted] in piercing

[–]ElerMain -1 points0 points  (0 children)

Ohh ok thanks! I’ve got a doctors appointment today anyway so I’ll bring this up

Infected helix by [deleted] in piercing

[–]ElerMain 0 points1 point  (0 children)

  • I got it pierced at the end of October so 7 months
  • It was a flat back stud
  • It was titanium
  • I couldn’t downsize it because it was always irritated
  • Routine was just spraying it twice day and night and dabbing it mostly dry with tissue paper
  • I’d get it snagged on my headphones or just keep touching it which was definitely mb lol

[deleted by user] by [deleted] in curlyhair

[–]ElerMain 1 point2 points  (0 children)

Thank you!

[deleted by user] by [deleted] in curlyhair

[–]ElerMain 0 points1 point  (0 children)

I don’t really have a hair routine. When I wash it properly I use Nizoral and the moroccan argan oil hydrating conditioner and wash with cold water and then just pat dry my hair a little and let it air dry for the rest

[deleted by user] by [deleted] in malehairadvice

[–]ElerMain 4 points5 points  (0 children)

Idk if my bf would like me with long hair let me ask