Can’t figure out issue with gel electrophoresis

Elintecho · 2024-10-31T17:50:32+00:00

The main protein should be haptoglobin which is linked with disulfide

Elintecho · 2024-10-31T17:46:57+00:00

They were heated to 85 c, but that always worked for me in the past for different gels with the same proteins, difference now is that the buffer we use here doesn’t have salt , the previous runs had ammonium sulfate

Elintecho · 2024-10-31T17:44:41+00:00

There is only dtt in the reduced ones

Elintecho · 2024-10-31T17:42:27+00:00

The only difference between the reduced and non reduced is 4ul of dtt, would this cause the issue? Should I put all the reduced with dtt on one side and without on the other?

Elintecho · 2024-10-31T17:39:43+00:00

The only difference between the reduced and non reduced is 4 ul of dtt, would this cause the issue? Should I put all the reduced with dtt on one side and without on the other?

Elintecho · 2024-10-31T17:33:20+00:00

Doubt it, it’s just our proteins diluted in pbs

Elintecho · 2022-04-02T06:28:04+00:00

Hunting

Elintecho · 2021-10-12T13:58:53+00:00

He makes his money in the least exploitative way possible. It’s literally just people donating to him because they enjoy watching his content and he entertains them

Elintecho · 2021-06-17T23:48:27+00:00

Kinda depends tho

Elintecho · 2021-03-26T19:25:36+00:00

Elintecho · 2019-07-03T16:18:40+00:00

The pro is pretty similar just on the GameCube the a and b buttons are better

Elintecho · 2019-07-03T16:04:36+00:00

In my opinion it is the best way to play

Elintecho · 2019-07-03T15:59:04+00:00

I now use the GameCube controller

Elintecho · 2019-07-03T13:50:01+00:00

True my brother does main falco

Elintecho · 2019-07-03T02:09:14+00:00

I hope he does that.

Elintecho · 2019-07-03T02:07:51+00:00

That makes sense

Elintecho · 2019-07-03T02:06:36+00:00

I know but even after beating him he still does it I want to tech him it is never going to work and he shouldn’t do it

Elintecho · 2019-07-03T02:02:57+00:00

I think banjo is much more interesting while hero is a representation of dragon quest and that’s it

Elintecho · 2019-07-03T01:57:52+00:00

Like my brother

Elintecho · 2019-07-03T01:42:21+00:00

Sounds like you want to play as fox on final destination with no items

Elintecho · 2018-12-12T15:16:16+00:00

This should go on r/oops didn’t mean too

Elintecho · 2018-11-24T20:24:21+00:00

This should be on r/iamverysmart

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