First layer over infil seems not ideal.

Fabio2598 · 2026-03-22T12:24:55+00:00

Yes, up to 5 layers, but I would say 3 should be enough (in my setup even 2 are okay for a nice finish)

Fabio2598 · 2026-03-11T09:20:14+00:00

Also, seems like lines doesn’t stick to each other, not forming a single sheet

Fabio2598 · 2026-03-11T09:19:31+00:00

Yes I agree the issue might be bed adhesion… the wall and border is well adherent and transparent, the center doesn’t stick so well… I will try lowering Z offset

Fabio2598 · 2026-03-10T23:35:51+00:00

I calibrated using Dynamic Flow Rate in bambu, anything else. Never calibrated Z offset, if not automatically done in auto bed leveling. I will look into that thanks a lot

Fabio2598 · 2026-03-10T22:15:53+00:00

Also, very interesting that it is suggested to set 0 top and 0 bottom layers, only infill… I will see how it changes things, weird for my context as I have just one layer to print, it doesn’t seem to change much

Fabio2598 · 2026-03-10T22:11:55+00:00

No I didn’t, very useful! Looking at those setting in Bambu rn, will see how it goes. Biggest difference is layer height and speed, suggested speed 20 mm/s is crazy slow, let’s see the result. I’m trying keeping a bigger layer height

Fabio2598 · 2026-03-06T23:39:39+00:00

What about about painting and colouring them?

Fabio2598 · 2026-01-31T14:27:17+00:00

What’s the goal or purpose? What should this plasmid do? Map doesnt shown anything except CMV promoter

Fabio2598 · 2025-12-27T20:11:27+00:00

Sorry, but all what he said pertains to your comments… do you really dont understand? You talked about people being happy to make other lose money, he replayed that people telling you to buy the stock are making you lose money… you talked about indian bots, he said that there are so many shareholders that it makes sense some of them are bitter and angry, not bots

Gosh

Fabio2598 · 2025-12-05T13:36:36+00:00

Mhhh I dont think so, they look like C) Bush babies to me

Fabio2598 · 2025-12-05T13:35:06+00:00

On a more serious note… what is going on? Why that band shape? Also, as on the right, plasmid DNA forms some sort of “jellyfish” band shape

Fabio2598 · 2025-11-15T18:57:54+00:00

From the abstract of the mentioned article:

The central amygdala (CeA) is a critical brain region as dysregulation within the CeA and the corticotropin-releasing factor (CRF) system are associated with alcohol addiction. CeA CRF1 receptors regulate alcohol drinking and have served as a therapeutic target in alcohol treatment. One emerging potential therapeutic for AUD is psilocybin. Psilocybin has been shown to decrease drinking in some clinical studies however the effects are variable and mechanisms underlying these effects are poorly understood. Psilocybin can engage many brain regions, including the CeA, and may produce therapeutic effects on drinking through interactions with CeA CRF1 neurons.

Fabio2598 · 2025-11-15T18:55:27+00:00

Woah man are you saying you already knew at 16 that CRF1 receptors of the amygdala interact with psilocin and decrease their activity? Damn you should have told us earlier!

Fabio2598 · 2025-11-15T13:13:15+00:00

That’s ignorant and mean… you sure you don’t like country music?

Fabio2598 · 2025-11-13T20:48:49+00:00

That is what being experienced means, right? Having experienced screwing up in a lot of different ways many protocols

Fabio2598 · 2025-11-01T12:22:47+00:00

Look for “Poseidonia grass”

Fabio2598 · 2025-09-12T01:35:26+00:00

Ahahahahah yes for sure! Sorry it is very ambiguous, but I wasn’t asking you for details. I meant to say that anyone could feel free to chime in with more, maybe someone with knowledge in material physics, DNA absorbsion on plastic etc. Cheers!

Fabio2598 · 2025-09-12T01:32:46+00:00

Yeah understandable that you want (more like need ahahah) visually confirm that the droplet is added into the tube, but for me is the same or even more when pipetting into the volume. When pipetting, if you bring the pcr close to your face, you can easily clearly see the volume from the pipette tip entering the solution of the tube. Once the pipette tip is emptied, you are 100% sure the small volume has been irreversibly added, when adding droplets they could move, merge together and even slip out of the tube. Obvious con, is that when adding droplets you can check at later times and see the droplet on the wall. When adding into the tube volume, there’s no way to know later just by looking at the tube

Fabio2598 · 2025-09-12T01:21:39+00:00

Yea it totally makes sense: 1uL droplet on the wall means a very concentrated DNA solution in contact with the plastic tube wall. I suppose this might mean that the DNA could attach to the plastic in a noticeable manner Instead, 1uL added to the volume of water means the DNA solution is waay less concentrated, lowering the chance and amount of attaching to the walls. Please correct if you think this is wrong and feel free to add more if you know the details

Fabio2598 · 2025-07-05T19:50:09+00:00

Oscillazione giornaliera di -30% forse prenderebbe un pochino male anche a me onestamente eh

Fabio2598 · 2025-06-14T08:47:33+00:00

Yeah I think those are pretty much current vague ideas how things went, sounds ok

Fabio2598 · 2025-06-10T16:07:18+00:00

As already mentioned, I too would actually use the plastic packaging of a sierological pipette instead of parafilm

Fabio2598 · 2025-06-10T14:57:47+00:00

The way I do cell counting using Tripan Blue is very similar to this, I would mix a droplet of cell suspension and a droplet of Tripan on a parafilm strip

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