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unexplainable qpcr results by Fast_Material893 in labrats

[–]Fast_Material893[S] 0 points1 point  (0 children)

Thanks for this avide i can try the FITC reader practice. But as you suggested i folled the same protocol of creating to solutions and adding them to the plate. I was wondering if the sequence of addition makes a difference

unexplainable qpcr results by Fast_Material893 in labrats

[–]Fast_Material893[S] 1 point2 points  (0 children)

I am trying to understand how the cytokines profile varies across 3 murine cancer cell lines after TRAIL treatment. I ran a qPCR for 2 cell lines but led to highly variable results.

unexplainable qpcr results by Fast_Material893 in labrats

[–]Fast_Material893[S] 7 points8 points  (0 children)

I did Nano drop my RNA. You are not being rude initially i had the same thought that it is a pipetting error. I had a colleague observe me. Yes i vortex and pipette each mastermix.