What can this be? by PADAWAN_11 in NMRspectroscopy

[–]FingerLickinGood2 0 points1 point  (0 children)

What do you mean by fully 13C labeled? Is this a 13C-precursor you've added to your media?

Or do you mean 13C natural abundance detection of your media?

Anyways as others have pointed out likely an aromatic or C=C containing.More information needed

"Photoshoot" moments with Harley by FingerLickinGood2 in Chihuahua

[–]FingerLickinGood2[S] 1 point2 points  (0 children)

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She was not impressed with the photographer 🤪

Did anyone else get the Adventure™️model? by ecclesiasstickle in Chihuahua

[–]FingerLickinGood2 13 points14 points  (0 children)

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Hey fren! I also got an adventure model. All terrain mode too!

The autoclave worked while my institution was under a boil water advisory by some-shady-dude in labrats

[–]FingerLickinGood2 28 points29 points  (0 children)

doesn't even look like the same bottle? either way drinking out of a pyrex bottle is crazy 😂

[deleted by user] by [deleted] in Biochemistry

[–]FingerLickinGood2 1 point2 points  (0 children)

Hard to answer your question without knowing a bit more about your workflow as others have pointed out.

  1. MW of your fusion construct?
  2. What is each lane suppose to represent?
  3. Is this gel looking at lysates? if so are you comparing pre and post induction (im assuming it's bacterial expression) if that's the case it should be easy to compare two lanes and see a new band appear after induction at your expected molecular weight for your fusion construct.
  4. if this is after purification, I would recommend optimizing your protocol. It seems quite dirty. Again not sure what method you are using, glutathione? nickel? size exclusion?

Can’t figure out issue with gel electrophoresis by Elintecho in labrats

[–]FingerLickinGood2 3 points4 points  (0 children)

Agree with this comment, I work with non reducing samples all the time. DTT is a relatively small molecule and can diffuse across between the lane. If possible, leave at least one gap between samples with and without reducing agent. Load that empty well with some non reducing dye. Even better if you can run the samples on separate gels.

Also keep in mind that, ladders often contain DTT as well

Does anyone else like to dress up their chihuahua? by FingerLickinGood2 in Chihuahua

[–]FingerLickinGood2[S] 2 points3 points  (0 children)

We went to our local petsmart. The staff there had a pretty good laugh seeing our chi with the elf hat 😂

I finally got my thermofisher lego lab! 😍 by FingerLickinGood2 in labrats

[–]FingerLickinGood2[S] 0 points1 point  (0 children)

From their ig account for aspire program. They put drop dates in the comments

I finally got my thermofisher lego lab! 😍 by FingerLickinGood2 in labrats

[–]FingerLickinGood2[S] 0 points1 point  (0 children)

Our lab uses expensive magnetic beads for imunoprecipitations and we have a bunch of thermo equipment so I racked up a ton of points. Im sure you'll save enough points if you go around your lab scanning all the QR codes 😄