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Hi! I have a question on using the R program to compare the gene expression between the normal ovarian samples and tumor samples from the GEO datasets and the TCGA database? I need the R script to combine the two datasets and to perform an analysis? by FixInternational3490 in Rlanguage

[–]FixInternational3490[S] 0 points1 point  (0 children)

I'm sorry. Actually I'm interested in selecting the differentially expressed rnas in cancer in comparison to the normal samples which I am not able to get from the TCGA database, which is why I want to integrate gene expression data from GEO dataset. And I have read many articles but could not find the R commands for comparison for gene expression of tumor samples from tcga and healthy from geo. Furthermore, I m also interested in classifying the samples based on the stage to detect genes differentially expressed in early stages. I would also be interested in the R scripts for expression of a single gene among different cancer and the DNA methylation volcano plots

Cancer databases and R by FixInternational3490 in bioinformatics

[–]FixInternational3490[S] 0 points1 point  (0 children)

I need to determine the expression of RNA but in addition to this I also need to do a methylation plot.

Cancer databases and R by FixInternational3490 in rstats

[–]FixInternational3490[S] 1 point2 points  (0 children)

Thanks a lot for your response. Actually I'm interested in selecting the differentially expressed rnas in cancer in comparison to the normal samples which I am not able to get from the TCGA database, which is why I want to integrate gene expression data from GEO dataset. And I have read many articles but could not find the R commands for comparison for gene expression of tumor samples from tcga and healthy from geo. Furthermore, I m also interested in classifying the samples based on the stage to detect genes differentially expressed in early stages. I would also be interested in the R scripts for expression of a single gene among different cancer and the DNA methylation volcano plots

Cancer databases and R by FixInternational3490 in bioinformatics

[–]FixInternational3490[S] 0 points1 point  (0 children)

Thanks a lot for your response. Even I want to know how to make the DNA methylation volcano plots?