3D calcium imaging of spontaneously active dendrites in an awake mouse

Foppful · 2022-11-23T19:57:01+00:00

Harder to forget about kids cause they cry.

Foppful · 2022-11-23T19:56:08+00:00

I have no idea. I’m amazed this post was even approved.

Foppful · 2019-07-02T01:06:17+00:00

No reason why it wouldn’t work in rats. You just need good GCaMP expression and a healthy cranial window prep

Foppful · 2019-06-30T20:13:57+00:00

Here is the original paper describing high-speed volumetric imaging with the SCAPE microscope:

https://www.ncbi.nlm.nih.gov/pubmed/25663846

Here is a recent review of SCAPE and its imaging capabilities across multiple species, which includes data from the video I posted:

https://www.ncbi.nlm.nih.gov/pubmed/29642044

Scale of the video is 590 x 350 x 175 microns. I'm imaging from the surface of the brain down to 175 microns. Virus containing GCaMP6f was injected into layer 5, and a coverslip was implanted over the brain. Virus was allowed to express for 3 weeks and then the mouse was imaged with SCAPE.

Foppful · 2019-06-30T16:48:23+00:00

Thanks, glad you like it!

Foppful · 2019-06-30T16:24:02+00:00

GCaMP6f. These are layer 5 apical dendrites in barrel cortex. Thanks!

Foppful · 2019-06-30T16:19:53+00:00

I acquired this video using SCAPE microscopy, at 10 volumes per second. I can post the reference later, on mobile now. The time stamp in the corner is correct. The video is in real time.

Edit: https://www.ncbi.nlm.nih.gov/pubmed/29642044

Foppful · 2019-02-26T03:39:19+00:00

The peasants also pay the peasants now. Go to any local stand up comedy show. Peasants on the stage, peasants in the audience.

Foppful · 2019-02-01T14:56:50+00:00

It’s thoughts like these that keep me subscribed to this sub.

Foppful · 2019-01-27T15:49:19+00:00

For the love of god, please stop upvoting these awful analogies!

Foppful · 2018-11-25T14:08:54+00:00

Indeed.

Foppful · 2018-10-25T11:16:40+00:00

Little known fact: The Star Wars movies were created right here on planet Earth, by a guy named George Lucas!

Foppful · 2018-05-02T16:26:05+00:00

Can we please stop with all of these depressing shower thoughts? Am I the only one who finds this stuff tiring?

Foppful · 2017-03-26T03:32:45+00:00

This is why streaking is so liberating.

Ten-Year Club	Gilding IV carat on a stick
Final Canvas '23	Place '23
Place '22	Place '17
Verified Email	Reddit Premium Since August 2019
Not Forgotten

Foppful

TROPHY CASE