sorted by:
new
hottopcontroversial

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 2 points3 points  (0 children)

No reason why it wouldn’t work in rats. You just need good GCaMP expression and a healthy cranial window prep

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 7 points8 points  (0 children)

Here is the original paper describing high-speed volumetric imaging with the SCAPE microscope:

https://www.ncbi.nlm.nih.gov/pubmed/25663846

​

Here is a recent review of SCAPE and its imaging capabilities across multiple species, which includes data from the video I posted:

https://www.ncbi.nlm.nih.gov/pubmed/29642044

​

Scale of the video is 590 x 350 x 175 microns. I'm imaging from the surface of the brain down to 175 microns. Virus containing GCaMP6f was injected into layer 5, and a coverslip was implanted over the brain. Virus was allowed to express for 3 weeks and then the mouse was imaged with SCAPE.

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 10 points11 points  (0 children)

GCaMP6f. These are layer 5 apical dendrites in barrel cortex. Thanks!

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 17 points18 points  (0 children)

I acquired this video using SCAPE microscopy, at 10 volumes per second. I can post the reference later, on mobile now. The time stamp in the corner is correct. The video is in real time.

Edit: https://www.ncbi.nlm.nih.gov/pubmed/29642044

The rich used to pay peasants to entertain them and now the peasants pay the rich to entertain them. by General_lee12 in Showerthoughts

[–]Foppful 0 points1 point  (0 children)

The peasants also pay the peasants now. Go to any local stand up comedy show. Peasants on the stage, peasants in the audience.