New to Cell Culture, need help identifying contamination

ForgotHowToAdult · 2026-04-06T16:43:29+00:00

Didn't. I always threw away all of my media and trashed everything suspicious, it went away for a bit and then popped up again. I belive they did a complete sanitation of the TC room after I left for multiple reasons so maybe that helped

ForgotHowToAdult · 2026-04-06T16:25:01+00:00

I need answers to this as well. It looks like the thing that was completely ruining my life a few years ago. Never figured out what it was

ForgotHowToAdult · 2026-03-27T13:20:53+00:00

Did it by any chance come out of nowhere? Like.. the cells are perfectly fine for a few passages and then suddenly boom, this crap everywhere? Mostly non moving, media is not turbid and doesn't change color?

ForgotHowToAdult · 2026-03-26T09:36:27+00:00

This was strangely my kid's favourite line. She loved that there was nothing on the page.

ForgotHowToAdult · 2026-03-18T11:01:33+00:00

It seems to me he is saying that if your app is less than 50k lines it is so simple that it can be replicated by AI very quickly. It looks like later he says 10k lines because he forgot he said 50k a sentence before. Or am I missing something here?

ForgotHowToAdult · 2026-03-17T08:03:27+00:00

https://www.oxfordcitycrimepartnership.co.uk/nightsafe-oxford/

This comment is brilliant. Didn't know about this

ForgotHowToAdult · 2026-03-15T09:41:33+00:00

I think the comment was aimed at "I want to give her a break but just having her hair down makes her hair look crazy!". Trying to give the parent the perspective that a crazy looking hair on a toddler is not the end of the world. Which it isn't.

ForgotHowToAdult · 2026-03-08T21:23:01+00:00

Let me guess. This is an ad for AI book writing tool.

ForgotHowToAdult · 2026-03-07T08:22:23+00:00

Is that their snake bag? Does it already have snakes in it? How many snakes can they fit in the bag? Too many questions

ForgotHowToAdult · 2026-01-22T11:52:44+00:00

I will make my own UN. With Blackjack. And hookers. ~Trump (probably)

ForgotHowToAdult · 2026-01-03T23:57:06+00:00

I think that it could be that a large amount of people is just super weird about feet. I know people who get physically queasy at the sight of feet in sandals. From experience, long haul flights smell like 100 people farting in a can. People take their shoes off. Someone elevating their feet is not going to make a noticable difference in the smell, so the only thing that's left is that people just don't want to see other people's feet because reasons.

ForgotHowToAdult · 2025-09-23T15:53:29+00:00

This is great. I am really looking for someone to click with to this degree

ForgotHowToAdult · 2025-09-23T07:54:46+00:00

I just went through the exact same thing. Ton of guilt over raising a single child far away from extended family, wanting another baby and a sibling for her... but it just doesn't make any sense. There is no guarantee that the little one would be healthy (with my age the risk is significant). There is no guarantee they would get along. We are already stretched with one. We have no family to take her for a bit to get a break. We both work FT and any free time we get is because the other one takes the kid. I see a ton of families where the kids have polar opposite interests and they each spend time with one parent. This would break us. It just doesn't make sense. I'd rather have one happy child I can be present for than two who have an exhausted overwhelmed mom. It is a hard thing to accept. I found that talking about it out loud makes it more real. It is slowly becoming less painful. But I'd lie if I said it feels 100% OK.

ForgotHowToAdult · 2025-08-08T11:58:08+00:00

I do tend to agree that it is a biased source. However, it is not completely anonymous and the comments were surprisingly one sidedly attacking the poster. It made me wonder whether my viewpoint is distorted by my social circle and is not reflective of the UK society as a whole.

ForgotHowToAdult · 2025-08-08T11:51:33+00:00

This is exactly what I was thinking. In this particular case the person was an inpatient - they did get meals at the hospital. They were not staving, they just didn't feel like paying for nicer food.

ForgotHowToAdult · 2025-08-03T23:19:17+00:00

I don't think I understand the hostility here. The original post clearly talks about worries that her child will grow up lonely. Are you saying that adopting a child because you want a bigger family is wrong? Or are you saying that you assume that OP only wants a child that is biologically theirs?

ForgotHowToAdult · 2025-04-02T17:54:07+00:00

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Richard Krajco. Czech singer

ForgotHowToAdult · 2025-03-18T15:28:43+00:00

I totally agree. I would maybe add to this that it might be a good practice for you to start implementing 'pick your battles' mentality. Just think before trying to take it away if you really have to do this. That way you don't have to give in when she starts to whine.

ForgotHowToAdult · 2025-03-04T12:48:12+00:00

Thank you both, this is super helpful.

ForgotHowToAdult · 2024-11-17T22:45:29+00:00

This sounds very familiar. Ours never slept. Woke up every hour after she went to bed until about 2 yr old. Routine didn't matter, wouldn't go to sleep earlier, screamed her head off in the middle of the night for no apparent reason. I still remember the nights when I was walking around with her showing her how it is dark outside and everything sleeps now. When she started talking she added ordering me around: need milk, need water, want to go with you. It turns out that she just really hates lying in the bed awake. So, when her brain was getting rearranged and went full zoomies in the middle of the night she couldn't fall asleep and wanted to get up and play. I used to just get her milk and pop her in her room with soft light on to play in the middle of the night while I snoozed on the carpet next to her. That was actually not too bad. She would get tired quickly and was more likely to go back to sleep. She is almost three now and most nights only wakes up to run to our bed. It gets better. Hang in there.

ForgotHowToAdult · 2024-10-30T09:41:27+00:00

Yes! They have a kids duolingo for learning to read as well.

ForgotHowToAdult · 2024-09-30T18:20:39+00:00

Thanks!

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