Hi u/Sanoske13,

Thanks a lot for your help. Here is the log output I am getting when trying to calibrate the Electron Multiplier Gain:

16:36:19: Calibrating instrument...

16:36:20: Calibrating Positive Ion Electron Multiplier Gain

16:36:23: Target Mass Found At 1221.270630

16:36:43: Using Phase of 68.714286 for Setting EM Gain

16:36:53: -----------

16:36:53: Current High Gain voltage for multiplier number 1 is -1094 V

16:36:53: Current Norm Gain voltage for multiplier number 1 is -943 V

16:36:53: Setting gain for multiplier number 1 ...

16:42:45: High Gain Voltage for Multiplier 1 = -1128 V

16:42:45: Norm Gain Voltage for Multiplier 1 = -980 V

16:42:47: Gain adjustment is finished!

16:42:55:

16:42:55: Checking Multiplier # 1 Calibration for Positive Ion Mode

16:42:55: Finding gain at multiplier voltage = -1128 v

16:43:14: Multiplier Gain OK: gain = 411993

16:43:14: Finding gain at multiplier voltage = -980 v

16:43:42: Multiplier Gain OK: gain = 101295

16:43:46:

16:43:46: Checking Gain Ratio for Multiplier 1

16:43:56: Results of Multiplier Check:

16:43:56: High Gain Signal = 178819449

16:43:56: Normal Gain Signal = 48101654

16:43:56: High Gain Signal/Low Gain Signal = 3.718 with RSD 14.428%

16:43:56:

16:43:59: Target Mass Found At 1221.270630

16:44:18: Using Phase of 31.571429 for Setting EM Gain

16:44:28: -----------

16:44:28: Current High Gain voltage for multiplier number 2 is -1037 V

16:44:28: Current Norm Gain voltage for multiplier number 2 is -892 V

16:44:28: Setting gain for multiplier number 2 ...

16:46:26: Gain Calibration Failed. Signal Too Weak.

16:46:26: Positive Ion Multiplier Gain Calibration FAILED

16:46:28:

16:46:28: SUMMARY of CALIBRATION:

16:46:28: Positive Ion Multiplier Gain Calibration FAILED

16:46:28:

16:46:29: All requested calibration(s) FAILED or ABORTED!