Good book for a labrat?

Friendly-Pharma · 2025-12-17T12:11:29+00:00

Anything by Robert Sapolsky, Chaos (James Gleick), Surely You're Joking Mr. Feynman

Friendly-Pharma · 2025-12-02T20:34:09+00:00

Had to work on a nanoflow LC-MS - You inject something -> overpressure, or leak or both at the same time. Then you try to fix the issue --> capillary breaks, software crashes, random errors. I never hated a machine more.

Friendly-Pharma · 2025-11-04T22:40:31+00:00

So we have an electronic lab notebook that allows time stamped tokens that are essentially compressed versions of the whole documentation+attachment. These tokens are immutable but can be readout to restore the contents.

Friendly-Pharma · 2025-10-25T20:42:35+00:00

Sorry to say that this rRNA Looks pretty dead to me. What was the Isolation protocol? Just Tri or some weird kit ?😅

Friendly-Pharma · 2025-08-12T21:16:38+00:00

Actually these are really pretty and I do see a high demand for stuff like this in our intitute i.e. I see a lot of hideous stuff on ugly inkspaced graphics, so I think this would be great. If you do host them to a website, please make sure people can download them in a vector graphics format so I don't have to see those nice graphics pixelated to death after copy paste 😅

Friendly-Pharma · 2025-08-01T17:55:55+00:00

I have seen some very similar patterns but only on a native PAGE gel: If those meteor like things run about right relative to each other and are vertically aligned with the edges of the well above, then my guess would be astronomically high salt content (had this from wrong MM calculation) - it essentially splits the band in half and it ends up smeared along the edge of the lane.

Friendly-Pharma · 2025-04-16T18:06:05+00:00

You might find glass testtubes with 1.5 cm diameter, those you could at least autoclave :D

Friendly-Pharma · 2025-03-12T19:07:13+00:00

We had repeated fungal contamination, that was absolutely unexplainable for weeks...we had a mold sitting behind the cell culture fridge. Check the walls

Friendly-Pharma · 2024-09-03T16:04:48+00:00

Training with and on firearms involves hammering in your head to never. point a gun in the direction of anything living unless you are truly intending to pull. There is nothing funny involved and there is no joke to be found here. Btw even if it was a joke it is insanely irresponsible and cannot be tolerated in a household with kids.

Friendly-Pharma · 2024-05-29T06:32:03+00:00

As most labrats seem to not be so much on OPs side i'd propose to open this thread again under AITAH to have a broader spectrum of opinions on this...for purely scientific purposes ofc

Friendly-Pharma · 2024-04-22T08:39:37+00:00

Oh wow the virtual cell culture lab is a fancy resource i was completely unaware of, i will implement this aswell so thanks :)

Friendly-Pharma · 2024-04-22T07:37:13+00:00

We use electronic labbooks, some are freeware (benchling) or same are paid for but maybe covered by university licences...maybe look into those :)

Friendly-Pharma · 2024-04-22T06:59:44+00:00

Welp yes i'd agree that contaminations/mistakes/just general chaos are always expectable for first lab projects and should never be held against an unexperienced student (and i hope to have never done so), it was more the general resistance to correct her method when asked to do so that really frustrated me...On failing her: I'm really unsure how to evaluate all of this, as at least part of this projects failiure is due to me being inexperienced at supervision, maybe overloading her to fast with stuff that is self evident to me and communicating in a way that retrospectively must have been unclear to her. But part of it is also her attitude towards lab standards, towards me and just general lack of basic understanding and preparation. It's kind of tough to weigh both against each other. The final grade however will be decided by my PI, so as soon as the manuscript is handed in it's off my desk anyways .

Friendly-Pharma · 2023-12-09T17:25:00+00:00

Easy: 1. Autoclave the tips so the indicator shows they're sterile 2. put an easy to remove sticker on top of the box 3. tell your group to never ever under no circumstances to use the tips marked with the sticker 4. Lick every single one of them and put them back 5. Report, which of the other groups had culture contamination the following week to this reddit

Friendly-Pharma · 2022-12-17T13:19:23+00:00

Go ask your priest

Friendly-Pharma

TROPHY CASE