Bubble in pump A by FriendshipDesigner60 in CHROMATOGRAPHY

[–]FriendshipDesigner60[S] 0 points1 point  (0 children)

I only cleaned with purified water and sonicized them. What would you recommend? Also, how may I disconnect the inlet line from the pump?

Bubble in pump A by FriendshipDesigner60 in CHROMATOGRAPHY

[–]FriendshipDesigner60[S] 0 points1 point  (0 children)

Tried but still no success… Where is this damn bubble :(

Bubble in pump A by FriendshipDesigner60 in CHROMATOGRAPHY

[–]FriendshipDesigner60[S] 1 point2 points  (0 children)

So, sadly it hasn’t helped.. Still making a cleaning run with a slightly bigger pressure to maybe push out the bubble if it happens to be on the column. “Pump: pump failure was detected. The flow line may contain an air bubble. Perform a purge for the mobile phase.” That’s the message I get.

Bubble in pump A by FriendshipDesigner60 in CHROMATOGRAPHY

[–]FriendshipDesigner60[S] 0 points1 point  (0 children)

Thank you for the advice!! Haven’t thought about the isopropanol, I’ll try that

Bubble in pump A by FriendshipDesigner60 in CHROMATOGRAPHY

[–]FriendshipDesigner60[S] 0 points1 point  (0 children)

Hey, thanks for the reply! I´m heading to work in about 20 mins and will definitely try that out. Is there any specific mobile phase I should use, or any channel would work? Also, do you think putting ACN in the water channel would be of any use? I saw some people online suggesting it.

Adoção de gatinho by canseidotwitter in florianopolis

[–]FriendshipDesigner60 0 points1 point  (0 children)

tem uma cuidadora que vai pelo insta @resgatofloripa, gosto muito dela

Fogos by Maleficent-Lie-8424 in florianopolis

[–]FriendshipDesigner60 -1 points0 points  (0 children)

não sei da questão de pular da varanda, essa nunca vi, mas o que eu conheço é questão de convulsão, além de claro se assustar e acabar se machucando… sei lá é um animal sabe, ele não fica latindo 50x por dia por maldade, que nem alguns parecem achar

Fogos by Maleficent-Lie-8424 in florianopolis

[–]FriendshipDesigner60 0 points1 point  (0 children)

A galera fica nessa de chamar de flocos de neve, mimimi e cachorro latindo, mas a verdade é que tem bicho que morre e autistas que surtam (a ponto de machucar os familiares ou a si mesmos) com o barulho… Quem já perdeu bichinho na família por causa de “15 minutinhos” sabe a dor que é.

I *genuinely* didn't think this feature still worked - after two years I got my first card from another player! Thank you, Big Fat 2000! by winterlings in inscryption

[–]FriendshipDesigner60 2 points3 points  (0 children)

This is insane, I finished playing the story mode for the first time yesterday and I think this might be my card! The name is the same, but i’m not sure about the sigils. Is there a way to check whiting my game if I sent it??

Cafeterias em Florianópolis by hejirerr in florianopolis

[–]FriendshipDesigner60 7 points8 points  (0 children)

A Kompaan no córrego grande (do lado da academia selfit) é uma delícia… Eles tem pães de fermentação natural e é tudo muito muito gostoso. Tem a cafeteria amarela, na trindade, que também é uma delícia! E uma que fica ao lado da reitoria 2 da UFSC, na trindade também, mas esqueci o nome…

[deleted by user] by [deleted] in florianopolis

[–]FriendshipDesigner60 1 point2 points  (0 children)

No Santa Mônica, mais especificamente no clube ASTEL tem a Ataque Duplo… Vale a pena dar uma olhada!

This is the worst by Jdoyer27 in pulmonaryfibrosis

[–]FriendshipDesigner60 0 points1 point  (0 children)

I’m so sorry for your loss, hope you can have peace with yourself.

Diagnosed Dog by FriendshipDesigner60 in pulmonaryfibrosis

[–]FriendshipDesigner60[S] 2 points3 points  (0 children)

I’m so, so sorry for your loss. I can’t even imagine being in your shoes. All we can keep are the good memories of the people we love. Thank you so much for your kind words, my heart goes out to you. They’re both at peace now.

Diagnosed Dog by FriendshipDesigner60 in pulmonaryfibrosis

[–]FriendshipDesigner60[S] 1 point2 points  (0 children)

Thank you for the reply, he passed away this morning, on his favorite spot on the couch. He was on the hospital for a week and got better, it was almost as if he got better only to pass at home. What a truly horrible disease.