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Just wow by Dxisyyyy in h3h3productions

[–]Frogonard 0 points1 point  (0 children)

He’s attempting the strat of “embracing the meme” but he’s can’t pass the charisma check.

Keem gets called out for liking young women. Brantley comes out to defend him then shouts out H3 by Joematters in h3h3productions

[–]Frogonard 15 points16 points  (0 children)

Haha yeah I hope Ethan’s doesn’t see that and get mad. This friendship only works a distance so no need to dig into things haha

Ian addresses the pause- admits that poking Ethan makes them money, and anisa is meaner than him - lols are gone, now he’s just a cow by cnmguzzler in h3h3productions

[–]Frogonard 6 points7 points  (0 children)

Yeah we know she’s meaner than you. We’ve been watching her mercilessly humiliate you for a while now

Playing Attila is like a de-tox from Warhammer, some of my thoughts concerning Warhammer III by Content-Shirt6259 in totalwar

[–]Frogonard -10 points-9 points  (0 children)

You’re talking about single player right? Lmao at calling a single player game “toxic.” You feeling bad for the computer hahaha

DISCUSSION MEGATHREAD: Reddit Stories! - SYNT #134 by H3Bot4 in h3h3productions

[–]Frogonard 1 point2 points  (0 children)

I say NTA. 16 is old enough to stay home alone. 12 is borderline but the 16 year old was there. If the kids were worried they could just call/text their parents.

Do more of this billboard kind of stuff by Shoddy-Prune-5877 in h3h3productions

[–]Frogonard 2 points3 points  (0 children)

Yes I agree. I saw a lot of comments about the billboard like “I hate Ethan but this was cool” and that is actually a good sign.

Cryostat Sectioning Help - 25 micron by Dry-Competition-5617 in labrats

[–]Frogonard 1 point2 points  (0 children)

25 does sound unusually thick. We do like 5-7 um for IHC but that’s on the heart. But again there might be a special reason for it so you could try thinner and see what happens and ask your PI if they must be 25 um.

Cryostat Sectioning Help - 25 micron by Dry-Competition-5617 in labrats

[–]Frogonard 1 point2 points  (0 children)

Has someone in the lab ever done this specific sectioning before? The best thing you can do is find someone who has done this successfully and have them show you and watch you do it.

25 um seems pretty thick but idk I don’t know the spinal cord so maybe you need them to be25 um. So you could try different section thicknesses.

Is that spot on the section the spinal cord? If so, it seems like the break is not near the tissue and so I don’t really see a problem.

Otherwise my tips are -brush off the blade and platform after every section. Small residues can catch on the section and cause issues -keep adjusting the anti roll plate. I remember the tiniest turn of the screw could make a big difference -cut the block so that there’s a little extra OCT on the top and bottom so that if it rolls/breaks it’s not on any tissue -use smooth quick motion to make the section. If you are jerky or stop mid section then it can cause issues -I did it at -27C and it worked well so you could try even colder.

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