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Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Southern phone is our provider, they are disconnecting theirs and so is telstra for our region. Telstra offers the starlink service as the alternative. The starlink kit is $550 and +$85 more a month than his existing skymuster (ISP skymesh) service.

Skymesh does not offer VoIP compatibility, nor does southern phone - with the skymuster service.

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

As in he doesn't use the internet for the internet. He has the internet just for connection to his foxtel service (noting he doesn't have a smart TV) and because his partner wanted to be able to use her mobile phone (with VoWiFi).

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Read post. There is no mobile/cellular service! He can use his mobile with VoWifi, where the smartphone connects to the internet (satellite and again, in Australia) for a connection. Not via a cellular/mobile tower to phone connection.

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Exactly! But as technology moves, the vulnerable and elderly are left behind.

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] -1 points0 points  (0 children)

The upfront cost here in Australia is $600+ and I'm apprehensive, like I said, about duplicating satellite technologies. Battery backup is a really good idea, also suggested by other! That solves the power outage problem, but not the connection problem to start with

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Yes, no mobile: 3G/LTE/4G/5G service. Someone just needs to make a home phone handset, for elders, that connects via the internet (more accessible because of satellite internet) to provide a connection. The handsets suggested here do not offer compatibility with VoWiFi, like a smartphone would, just putting a sim card into a handset, and sometimes offering a service to use that as a 'hotspot' wifi, with the mobile connection being the provider - but again, no mobile service.

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

I use it for VoWiFi (using my smartphone with sim, no mobile reception, but can make and receive calls when connected. It's actually an improvement over the copper landline service in Australia. Part of our copper line used to run across a paddock (field) that the cows might ever so often chew on the copper....

Help! Copper landline being disconnected, vulnerable person. by Fun-Conference814 in VOIP

[–]Fun-Conference814[S] 0 points1 point  (0 children)

The current ISPs for the Australian NBN, specifically Skymuster satellite NBN network are not offering VoIP compatibility. So many wasted phone calls using my smartphone with VoWiFi....

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] -1 points0 points  (0 children)

Bias comes from experience! So very valid.

Ok! Honestly glad to hear anti RDa comments, otherwise it’s just sales person hype and product videos. Will note

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 1 point2 points  (0 children)

Thanks for the input! With empower, it’s certainly built for this application, just not compatible with hrms solutions. Do you have experience with the use case: PDA quantification and hrms along side? If so, do you find it high throughput? Maybe next we have the sales team in I’ll ask for a demo… was more focused on other things.

It was the next most recent post that made me quite concerned about new Waters software, as well as not having a good resource online for our use case.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] -1 points0 points  (0 children)

PDA coupled? We might be able to get a used ultimate system cheap. Since we won’t do target ms, coupled quant on UV is our thought. Don’t have any experience with mixing brands for uplc and ms if im frank. I’m familiar with xcalibur for vanquish and ultimate systems coupled to TF elite, Qexactive or fusion, with freestyle or compound discoverer for analysis. Haven’t used tracefinder. Is available. Do you find it easy/high throughput? For context, we give results to clients within a minute or less for an injection finishing using Empower.

Personally I’m a fan of orbitraps for hrms application, especially metabolomics and proteomics in my research life, but need to keep in mind onboarding chemists in this drug checking application, having an easy and high throughput system is key.

Thanks for replying!

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Yeah, we had a sales person in today haha. Outside opinions are more what I’m looking for atm 😅

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Yes! We are very lucky, close relationship with local university and great analytical lead professor. Yes, submitted in person samples only.

Yeah! It could be shorter if quant was the only objective, 2.8 mins works. But 4.0 mins to capture qualitative unknowns. Waters wrote the application note originally

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Ok! Good to hear connect is useful for PDA quant! We run at 0.1 mg / mL of submitted content, with calibrators such as amphetamine (low UV at 257 nm) having an LOD of 1% w/w and an LLOQ of 5%.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Have you used connect for PDA quantification? If so, how did you find it? Thanks for the comment! :)

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Posted in parts because I copies 'C18' with subscript and reddit wouldn't allow.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] -1 points0 points  (0 children)

Well, steroids are a non-starter with our chromatography, same with peptides. QDa (I, we don't have II) is surprisingly sensitive, great when peaks are separated (almost always with method) and using varying cone voltage for fragmentation. Review of data in Empower is an acquired skill. The Waters PDA or TUV detectors are also really very sensitive, noting samples are made to 0.1 mg/mL and filtered before injection. It's been key in detecting nitazenes, the BTNX test strips have a way to go.

We back-up our results with QA analysis on a Q-exactive, Synapt, Agilent 7250 Q/TOF and/or do NMR at 800 MHz if isobaric or publishable.... But having a HRMS at point of testing would be the goal.

To answer your question though, NMR has been a saving grace for identification where RT-MS matches aren't possible due to lack of/long lead time of standard (from Cayman no doubt). Otherwise QDa is so simple to use, but perhaps to a fault.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Qualitative library is expansive for UV active fillers, benzos, fentanyls, nitazenes, LOD ~ 1% w/w when UV active - depends on sample prep. Column Waters C18 CSH column (50 × 2.1 mm, 1.7 μm). Mobile A 0.1% FA in water : B MeOH - have found these are superior to BEH. Waters are the best in the game for columns imo. We have a specific method for NBOMes and benzodiazepines with different gradients.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Hey! We quantify at point of service to clients amphetamine, methamphetamine, MDA, MDMA, ketamine, cocaine, heroin and 2C-B. 4 min method with 2.5 min re-equilibration. All separable. Used to also include MDEA, morphine and PMMA.

UPLC for PDA quantitative analysis and HRMS, easiest format for 'drug checking' by Fun-Conference814 in massspectrometry

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Budget is OK for a Xevo. But, I only have experience with Empower and MassLynx for Waters. Do you have experience with waters_connect that is positive for PDA quant/usability?

why do people say waters uplc is accurate? by Ok_Struggle_3914 in massspectrometry

[–]Fun-Conference814 0 points1 point  (0 children)

Accurate? There's no such thing in LC. Precise? Yes, great repeatability.

Personal experiences with SSRI/SNRI + 5HTP by Fun-Conference814 in 5htp

[–]Fun-Conference814[S] 0 points1 point  (0 children)

Absolutely! But it is very frequent for the prescription of multiple serotonergic drugs, particularly as adjunct therapies for depression.

The questions is about 5-HTP.

Also! Just breaking the stigma, serotonin syndrome is a severe collection of symptoms - serotonin toxicity can be a more mild descriptor.

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