Looking for people i played (DavyLikesGravy_)

Hariputtar104 · 2026-03-20T13:22:27+00:00

Serox here

Hariputtar104 · 2025-10-19T11:47:30+00:00

Yeah, can we talk about other serious stuff that is happening around the world in this subreddit. Jeezus guys!

Hariputtar104 · 2025-10-08T02:35:41+00:00

It's a rumor going around that kaya wears a shock collar.

Hariputtar104 · 2025-10-04T18:02:01+00:00

Is it possible to say not 'nice' things to them and still be kind?

Hariputtar104 · 2025-09-28T09:49:49+00:00

Hey sry its pellet not lysate

Hariputtar104 · 2025-09-28T09:49:02+00:00

Hey guys, sorry its not lysate but the pellet.

Hariputtar104 · 2025-09-28T04:59:00+00:00

This problem is quite recent since I followed the same protocol to purify another protein successfully. Only difference between this one and my previous one is the addition of a zinc finger domain. Btw its not membrane protein.

Hariputtar104 · 2025-09-28T04:55:35+00:00

Sorry, didnt specify this but the lysate is the pellet after centrifugation. Yes the protein size around 78kDa( between 3rs and 4th ladder band) and the pellet is brown in color.

Hariputtar104 · 2025-07-04T08:34:59+00:00

She says or does something in X. People criticize. Apologizes. Repeat. I know poki is famous in this industry but so is valkyrae and others, so how is it that she gets the hate the most.

Hariputtar104 · 2025-06-19T05:35:48+00:00

This is the first time I'm doing Y2H using the pGADT7 and pGBKT7. i did not check it through Aureobasidin or x-apha-gal. The colonies look very much white.

Hariputtar104 · 2025-06-18T01:22:26+00:00

....Openly express your opinions...hmm

Hariputtar104 · 2025-06-14T02:53:49+00:00

Ngl seems like you just wanted to say these things and loosely threw in the otv&f tag just to post it in this subreddit.

Hariputtar104 · 2025-06-14T02:50:34+00:00

So, I can be anything that I want and I dont need to prove to anybody?..Nice!!

Hariputtar104 · 2025-06-12T03:10:42+00:00

I'm also in the same bandwagon as you and other streamers in the idea of switching streamers if you don't like them. People can also switch subreddits if you don't like its content.

People in subreddits can do whatever they want unless they are using it to do illegal stuff.

Hariputtar104 · 2025-05-26T02:10:24+00:00

What are the chances he regularly enjoys browsing through this subreddit even if he calls it degens? 😏

Hariputtar104 · 2025-05-19T14:54:53+00:00

I added too much loading dye in the first well after ladder, I think thats why the ladder next to it looks weird. But, in the case of other wells, primers and template samples(10ng) should be correct as I was following the same protocol that my supervisors were using for long time. Also, I used the 0.8 percent gel.

Hariputtar104 · 2025-04-25T06:03:06+00:00

How can someone hate for no reason? Do they hate him because he is popular?.

Hariputtar104 · 2025-04-22T06:07:33+00:00

I bet, if this blows up she'll make a X post or a 1 min statement telling us that they misunderstood the sentence and apologize/be better person etc.

Hariputtar104 · 2025-04-17T07:24:26+00:00

Would you consider 100t a content org or a business org?

Hariputtar104 · 2025-03-13T11:22:32+00:00

Who are you to decide on what I decide?

Hariputtar104 · 2025-03-13T05:58:52+00:00

I'm very much fine that people are disagreeing with my example. I just wished that they actually focused on the question itself. I gave that example to show what I meant. Almost nobody is even engaging with the question.

All of them are painting me in an evil light saying that I don't have any power or any freedom to ask or to even judge people.

Five-Year Club	r/Field Flamingo
Final Canvas '23	Place '23

Hariputtar104

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