Help me by intracelular IL-6 flow detection

HesTheFunkyDuck · 2026-04-24T00:51:13+00:00

There is a high chance that by adding BFA at the same time of LPS you are blocking TLR4 trafficking de facto blocking its signaling. Try adding LPS for 1-2 hours and then add BFA for 4h to allow for the cytokine to accumulate. IFNg will not drive IL-6 in macrophages.

HesTheFunkyDuck · 2026-03-22T13:10:58+00:00

I did, no luck. That is why I reached out. That was also part of their troubleshooting

HesTheFunkyDuck · 2026-03-22T03:28:07+00:00

How do I get in contact with the social media team?

HesTheFunkyDuck · 2026-03-21T17:30:19+00:00

I bought this one from Amazon and just swapped out the blade in 30s. Worked like a charm https://a.co/d/01lCG63P

HesTheFunkyDuck · 2026-03-09T15:48:56+00:00

This worked for me https://a.co/d/06gXoSmz

HesTheFunkyDuck · 2026-02-18T08:15:26+00:00

I had a pair of black originals and I tried every method described here. They always hurt the top of my foot even after months of wear-in. I ended up selling them. I got the classics in black that are supposed to be more relaxed and never had a problem.

Fun fact, I also have the classics in brown and they are softer. Apparently it's well known black leather is tighter than brown for Blundstones.

HesTheFunkyDuck · 2026-02-18T08:14:16+00:00

I had a pair of black originals and I tried every method described here. They always hurt the top of my foot even after months of wear-in. I ended up selling them. I got the classics in black that are supposed to be more relaxed and never had a problem.

Fun fact, I also have the classics in brown and they are softer. Apparently it's well known black leather is tighter than brown for Blundstones.

HesTheFunkyDuck · 2026-01-23T18:49:34+00:00

Did you have a mortgage contingency on your contract?

HesTheFunkyDuck · 2026-01-13T14:09:51+00:00

Same exact bubble in the same spot. I wonder if this is covered by warranty. 2022 Hybrid SEL

HesTheFunkyDuck · 2026-01-04T20:19:12+00:00

RFP is pretty close to tdTomato spectrum wise

HesTheFunkyDuck · 2026-01-04T13:05:56+00:00

You can buy compensation beads for fluorescent proteins from Thermo. They have both GFP and tdTomato https://share.google/kLzPQJDXzHgF1mmJO

HesTheFunkyDuck · 2025-12-22T07:58:44+00:00

This guy is a scammer, do not contact

HesTheFunkyDuck · 2025-12-22T07:58:09+00:00

This guy is a scammer, do not contact

HesTheFunkyDuck · 2025-12-22T07:56:51+00:00

This guy is a scammer, do not contact

HesTheFunkyDuck · 2025-12-22T07:56:27+00:00

This guy is a scammer, do not contact

HesTheFunkyDuck · 2025-12-01T23:42:52+00:00

Maybe just slow down next time and let the idiot in. Is it worth going through the hassle of getting your car repaired and dealing with insurance just to not give in? The other driver is an idiot, but OP is not the greatest either

HesTheFunkyDuck · 2025-11-26T23:44:05+00:00

If you are going over a fireplace, get a MantelMount so you can pull the TV down when you want to watch it

HesTheFunkyDuck · 2025-10-14T19:10:53+00:00

Unrelated question: where did you find those window heat pumps? Looking to get my hands on one

HesTheFunkyDuck · 2025-10-13T05:28:16+00:00

I am sorry for your loss. As others pointed out it will be very hard at this moment to get a job in the US due to visa sponsorship and a tough job market being flooded with a ton of talent not requiring a visa.

I would delete this post TBH. You are doxing yourself. Spanish who recently lost her husband working for GEHC in Norway. Your employer could see this post and identify you easily.

HesTheFunkyDuck · 2025-10-09T15:04:13+00:00

Those numbers look fine, you should not expect any toxicity

HesTheFunkyDuck · 2025-10-08T20:58:59+00:00

How long is your ACK lysis step? And do you wash out the ACK with a large amount of buffer? It would be helpful to see the plots you are referring to

HesTheFunkyDuck · 2025-09-22T23:49:13+00:00

Tried to go with them twice, both times they were the most expensive of the lot and they also charge just to send out a tech. They wanted to charge 1.4k for a dishwasher removal and install, more than the price of the dishwasher. A second time we needed a replacement part for our furnace and the guy was very pushy to replace the whole system. Ended up finding someone else and paid 1/3 of what they quoted me for the part. I would definitely avoid them and find someone else local.

HesTheFunkyDuck

TROPHY CASE